ISSN:
1432-1424
Schlagwort(e):
cornea
;
endothelium
;
potassium channel
;
HCO 3 −
;
Cl−
;
anions
;
patch clamp
;
rabbit
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Summary The apical membrane of the rabbit corneal endothelium contains a potassium-selective ionic channel. In patch-clamp recordings, the probability of finding the channel in the open state (P o) depends on the presence of either HCO 3 − or Cl− in the bathing medium. In a methane sulfonate-containing bath,P o is 〈0.05 at all physiologically relevant transmembrane voltages. With 0mm [HCO 3 − ] o at +60 mV,P o was 0.085 and increased to 0.40 when [HCO 3 − ] o was 15mm. With 4mm [Cl−] o at +60 mV,P o was 0.083 and with 150mm Cl−,P o increased to 0.36. LowP o's are also found when propionate, sulphate, bromide, and nitrate are the primary bath anions. The mechanism of action of the anion-stimulated K+ channel gating is not yet known, but a direct action of pH seems unlikely. The alkalinization of cytoplasm associated with the addition of 10mm (NH4)2SO4 to the bath and the acidification accompanying its removal do not result in channel activation nor does the use of Nigericin to equilibrate intracellular pH with that of the bath over the pH range of 6.8 to 7.8. Channel gating also is not affected by bathing the internal surface of the patch with cAMP, cGMP, GTP-γ-s, Mg2+ or ATP. Blockers of Na/H+ exchange, Na+−HCO 3 − cotransport, Na+−K+ ATPase and carbonic anhydrase do not block the HCO 3 − stimulation ofP o. Several of the properties of the channel could explain some of the previously reported voltage changes that occur in corneal endothelial cells stimulated by extracellular anions.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01869382
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