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  • 1
    Online Resource
    Online Resource
    Quantification of spatial metal accumulation patterns in Noccaea caerulescens by X-ray fluorescence image processing for genetic studies
    Springer Science and Business Media LLC ; 2021
    In:  Plant Methods Vol. 17, No. 1 ( 2021-12)
    Details
    In: Plant Methods, Springer Science and Business Media LLC, Vol. 17, No. 1 ( 2021-12)
    Abstract: Hyperaccumulation of trace elements is a rare trait among plants which is being investigated to advance our understanding of the regulation of metal accumulation and applications in phytotechnologies. Noccaea caerulescens (Brassicaceae) is an intensively studied hyperaccumulator model plant capable of attaining extremely high tissue concentrations of zinc and nickel with substantial genetic variation at the population-level. Micro-X-ray Fluorescence spectroscopy (µXRF) mapping is a sensitive high-resolution technique to obtain information of the spatial distribution of the plant metallome in hydrated samples. We used laboratory-based µXRF to characterize a collection of 86 genetically diverse Noccaea caerulescens accessions from across Europe. We developed an image-processing method to segment different plant substructures in the µXRF images. We introduced the concentration quotient (CQ) to quantify spatial patterns of metal accumulation and linked that to genetic variation. Results Image processing resulted in automated segmentation of µXRF plant images into petiole, leaf margin, leaf interveinal and leaf vasculature substructures. The harmonic means of recall and precision (F1 score) were 0.79, 0.80, 0.67, and 0.68, respectively. Spatial metal accumulation as determined by CQ is highly heritable in Noccaea caerulescens for all substructures, with broad-sense heritability (H 2 ) ranging from 76 to 92%, and correlates only weakly with other heritable traits. Insertion of noise into the image segmentation algorithm barely decreases heritability scores of CQ for the segmented substructures, illustrating the robustness of the trait and the quantification method. Very low heritability was found for CQ if randomly generated substructures were compared, validating the approach. Conclusions A strategy for segmenting µXRF images of Noccaea caerulescens is proposed and the concentration quotient is developed to provide a quantitative measure of metal accumulation pattern, which can be used to determine genetic variation for such pattern. The metric is robust to segmentation error and provides reliable H 2 estimates. This strategy provides an avenue for quantifying XRF data for analysis of the genetics of metal distribution patterns in plants and the subsequent discovery of new genes that regulate metal homeostasis and sequestration in plants.
    Type of Medium: Online Resource
    ISSN: 1746-4811
    URL: Article
    DOI: 10.1186/s13007-021-00784-9
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2203723-8
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  • 2
    Online Resource
    Online Resource
    Actephila alanbakeri (Phyllanthaceae): a new nickel hyperaccumulating plant species from localised ultramafic outcrops in Sabah (Malaysia)
    Springer Science and Business Media LLC ; 2015
    In:  Botanical Studies Vol. 57, No. 1 ( 2015-12)
    Details
    In: Botanical Studies, Springer Science and Business Media LLC, Vol. 57, No. 1 ( 2015-12)
    Type of Medium: Online Resource
    ISSN: 1999-3110
    URL: Article
    DOI: 10.1186/s40529-016-0122-1
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2015
    detail.hit.zdb_id: 2432110-2
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  • 3
    Online Resource
    Online Resource
    Phyllanthus rufuschaneyi: a new nickel hyperaccumulator from Sabah (Borneo Island) with potential for tropical agromining
    Springer Science and Business Media LLC ; 2018
    In:  Botanical Studies Vol. 59, No. 1 ( 2018-12)
    Details
    In: Botanical Studies, Springer Science and Business Media LLC, Vol. 59, No. 1 ( 2018-12)
    Type of Medium: Online Resource
    ISSN: 1999-3110
    URL: Article
    DOI: 10.1186/s40529-018-0225-y
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2018
    detail.hit.zdb_id: 2432110-2
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  • 4
    Online Resource
    Online Resource
    Ecology of Paphiopedilum rothschildianum at the type locality in Kinabalu Park (Sabah, Malaysia)
    Springer Science and Business Media LLC ; 2015
    In:  Biodiversity and Conservation Vol. 24, No. 7 ( 2015-7), p. 1641-1656
    Details
    In: Biodiversity and Conservation, Springer Science and Business Media LLC, Vol. 24, No. 7 ( 2015-7), p. 1641-1656
    Type of Medium: Online Resource
    ISSN: 0960-3115 , 1572-9710
    URL: Article
    DOI: 10.1007/s10531-015-0881-0
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2015
    detail.hit.zdb_id: 2000787-5
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    SSG: 23
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  • 5
    Online Resource
    Online Resource
    Global Plant Ecology of Tropical Ultramafic Ecosystems
    Springer Science and Business Media LLC ; 2023
    In:  The Botanical Review Vol. 89, No. 2 ( 2023-06), p. 115-157
    Details
    In: The Botanical Review, Springer Science and Business Media LLC, Vol. 89, No. 2 ( 2023-06), p. 115-157
    Type of Medium: Online Resource
    ISSN: 0006-8101 , 1874-9372
    URL: Article
    DOI: 10.1007/s12229-022-09278-2
    RVK:
    WA 15000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2066026-1
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  • 6
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    Global research on ultramafic (serpentine) ecosystems (8th International Conference on Serpentine Ecology in Sabah, Malaysia): a summary and synthesis
    CSIRO Publishing ; 2015
    In:  Australian Journal of Botany Vol. 63, No. 2 ( 2015), p. 1-
    Details
    In: Australian Journal of Botany, CSIRO Publishing, Vol. 63, No. 2 ( 2015), p. 1-
    Abstract: Since 1991, researchers from approximately 45 nations have participated in eight International Conferences on Serpentine Ecology (ICSE). The Conferences are coordinated by the International Serpentine Ecology Society (ISES), a formal research society whose members study geological, pedological, biological and applied aspects of ultramafic (serpentine) ecosystems worldwide. These conferences have provided an international forum to discuss and synthesise multidisciplinary research, and have provided opportunities for scientists in distinct fields and from different regions of the world to conduct collaborative and interdisciplinary research. The 8th ICSE was hosted by Sabah Parks in Malaysia, on the island of Borneo, and attracted the largest delegation to date, 174 participants from 31 countries. This was the first time an ICSE was held in Asia, a region that hosts some of the world’s most biodiverse ultramafic ecosystems. The presentations provided a cross-section of the current status of research in all aspects of ultramafic-biota relations. In this Special Issue of Australian Journal of Botany (Issues 1–2 combined and 3–4 combined), we have compiled a selection of papers from among the oral and poster presentations to provide insights into recent advances in geoecological and applied studies of ultramafic habitats worldwide. Here we provide a preview of select papers found in this Special Issue and summarise some of the contributions made during the 8th ICSE and describe some of the exciting challenges awaiting future research.
    Type of Medium: Online Resource
    ISSN: 0067-1924
    URL: Article
    DOI: 10.1071/BT15060
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2015
    detail.hit.zdb_id: 1496155-6
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  • 7
    Online Resource
    Online Resource
    A systematic assessment of the metallome of selected plant families in the Queensland (Australia) flora by using X-ray fluorescence spectroscopy
    CSIRO Publishing ; 2023
    In:  Australian Journal of Botany Vol. 71, No. 4 ( 2023-5-5), p. 199-215
    Details
    In: Australian Journal of Botany, CSIRO Publishing, Vol. 71, No. 4 ( 2023-5-5), p. 199-215
    Abstract: Context Fewer than 10 plant species from Australia were known to hyperaccumulate metal(loid)s, despite metal-rich soils being widespread in Australia. By measuring herbarium specimens with non-destructive portable X-ray fluorescence spectroscopy (XRF) instrumentation their metal(loid)s concentrations can be determined, providing information that could be used to probe the evolution, biogeography, ecology, and physiology of plant species. Aims This study aimed to systematically measure herbarium specimens to obtain information on the prevailing concentrations of metal(loid)s in nearly 7000 plant specimens across seven plant families, and to link this data to an assessment of their spatial distribution. Methods The raw XRF spectrum of each herbarium specimen was processed using a new data-analysis pipeline recently validated for XRF data of herbarium specimens, to determine the concentrations of the first-row metal transition elements, and other detected elements. The collection localities of each of the herbarium specimens were plotted against rainfall and soil types to assess possible distributional patterns. Key results The results showed several newly discovered hyperaccumulator plant species, including 15 for manganese, two for nickel, three for cobalt, three for zinc, two for rare earth elements and one for selenium. Conclusions and implications Australia has more hyperaccumulator plant species than previously known and the XRF analysis of herbarium specimens is a powerful tool for their discovery. This research presents a new value proposition for the continued funding of herbarium collections in Australia and could initiate a range of research opportunities to use these data for future studies of plant evolution and adaptation.
    Type of Medium: Online Resource
    ISSN: 0067-1924 , 1444-9862
    URL: Article
    DOI: 10.1071/BT22028
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2023
    detail.hit.zdb_id: 1496155-6
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  • 8
    Online Resource
    Online Resource
    Corrigendum to: Metallophytes on Zn-Pb mineralised soils and mining wastes in Broken Hill, NSW, Australia
    CSIRO Publishing ; 2018
    In:  Australian Journal of Botany Vol. 66, No. 3 ( 2018), p. 286-
    Details
    In: Australian Journal of Botany, CSIRO Publishing, Vol. 66, No. 3 ( 2018), p. 286-
    Abstract: The wastes of metalliferous mining activities produce a substrate that is generally unfavourable for normal plant establishment and growth. However, metallophytes have evolved to grow in hostile environments that are rich in metals. They possess key properties that commend them for revegetation of mines and metal-contaminated sites. This field survey aimed to identify native metallophytes occurring on minerals wastes and mineralised outcrops in Broken Hill (New South Wales, Australia). Foliar concentrations of minerals were very high compared with non-mineralised soils but within the range expected for plants in such environments. Neither hyperaccumulators nor obligate metallophytes have been found, but they may be present on isolated mineralised outcrops in the wider Broken Hill area; however, a range of facultative metallophytes was identified in this study. These species could be introduced onto mining leases if establishment protocols for such species were developed.
    Type of Medium: Online Resource
    ISSN: 0067-1924
    URL: Article
    DOI: 10.1071/BT17143_CO
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2018
    detail.hit.zdb_id: 1496155-6
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  • 9
    Online Resource
    Online Resource
    Nickel distribution in
    CSIRO Publishing ; 2022
    In:  Australian Journal of Botany Vol. 70, No. 4 ( 2022-7-13), p. 304-310
    Details
    In: Australian Journal of Botany, CSIRO Publishing, Vol. 70, No. 4 ( 2022-7-13), p. 304-310
    Abstract: Context Hyperaccumulator plants are of considerable interest for their extreme physiology. Stackhousia tryonii is a nickel (Ni) hyperaccumulator plant endemic to ultramafic outcrops in Queensland (Australia) capable of attaining up to 41 300 μg g−1 foliar Ni. Aims This study sought to elucidate the distribution of Ni in S. tryonii by using synchrotron X-ray fluorescence micro-computed tomography (XFM-CT), complemented with elemental maps acquired from physically sectioned plant organs. Its Ni-enriched cylindrical photosynthetic stems make them particularly well suited samples for synchrotron XFM-CT. Methods XFM-CT enables ‘virtual sectioning’ of a sample, avoiding artefacts arising from physical sample preparation. The method can be used on fresh samples that are frozen during the analysis, which preserves ‘life-like’ conditions by limiting radiation damage. It also prevents/minimises other artefacts. Key results The results showed that Ni is mainly concentrated in the apoplastic space surrounding epidermal cells, and in some epidermal cell vacuoles. This finding is significant because this ‘free’ solute Ni is likely to be lost during physical sectioning. Conclusions and implications This case study has highlighted the utility of the XFM-CT approach for visualising metals within intact plant organs, which may be used across the plant sciences.
    Type of Medium: Online Resource
    ISSN: 0067-1924 , 1444-9862
    URL: Article
    DOI: 10.1071/BT22012
    Language: English
    Publisher: CSIRO Publishing
    Publication Date: 2022
    detail.hit.zdb_id: 1496155-6
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  • 10
    Online Resource
    Online Resource
    Assessing radiation dose limits for X-ray fluorescence microscopy analysis of plant specimens
    Oxford University Press (OUP) ; 2020
    In:  Annals of Botany Vol. 125, No. 4 ( 2020-03-29), p. 599-610
    Details
    In: Annals of Botany, Oxford University Press (OUP), Vol. 125, No. 4 ( 2020-03-29), p. 599-610
    Abstract: X-ray fluorescence microscopy (XFM) is a powerful technique to elucidate the distribution of elements within plants. However, accumulated radiation exposure during analysis can lead to structural damage and experimental artefacts including elemental redistribution. To date, acceptable dose limits have not been systematically established for hydrated plant specimens. Methods Here we systematically explore acceptable dose rate limits for investigating fresh sunflower (Helianthus annuus) leaf and root samples and investigate the time–dose damage in leaves attached to live plants. Key Results We find that dose limits in fresh roots and leaves are comparatively low (4.1 kGy), based on localized disintegration of structures and element-specific redistribution. In contrast, frozen-hydrated samples did not incur any apparent damage even at doses as high as 587 kGy. Furthermore, we find that for living plants subjected to XFM measurement in vivo and grown for a further 9 d before being reimaged with XFM, the leaves display elemental redistribution at doses as low as 0.9 kGy and they continue to develop bleaching and necrosis in the days after exposure. Conclusions The suggested radiation dose limits for studies using XFM to examine plants are important for the increasing number of plant scientists undertaking multidimensional measurements such as tomography and repeated imaging using XFM.
    Type of Medium: Online Resource
    ISSN: 0305-7364 , 1095-8290
    URL: Article
    DOI: 10.1093/aob/mcz195
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2020
    detail.hit.zdb_id: 1461328-1
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