In:
Health Risk Analysis, Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, , No. 3 ( 2020-9), p. 139-147
Abstract:
In Vietnam and around the world, Staphylococcus aureus remains a major hazard of food safety and food poisoning. S. aureus is present in many places and easily contaminates food production during processing chains. In this study, we successfully isolated S. aureus strains from suspected samples of two food borne poisoning outbreaks in Ha Giang and Vinh Phuc in 2017 and 2018, respectively. The collected samples were examined for presence of staphylococcal enterotoxins (SEs) by using 3MTMTECRATM Staph Enterotoxin kit, from there all the samples were positive with SEs. Different strains of S. aureus were isolated and then confirmed by MALDI-TOF technique. Those strains then were stored in Brain heart solution with 15% glycerol until further analysis. Our results identified three STs, ST96, ST88 (spa type t7558), and ST72 (spa type t3092), were responsible for two outbreaks. Two virulence genes detected from the above strains were sea and sec. Furthermore, these strains are test for antibiotic resistance susceptibility with commonly antibiotics. Penicillin are found to be resisted by all three STs, in particularly, ST96 and ST88 are both resistant to erythromycin while ST72 is resistant to gentamicin. Taken together, our study highlights the usefulness of molecular characterization to study and monitor bacterial pathogens associated with food poisoning outbreaks in Vietnam.
Type of Medium:
Online Resource
ISSN:
2308-1155
,
2308-1163
Uniform Title:
Выделение и описание Staphylococcus aureus, вызвавшей две широкомасштабные вспышки пищевых отравлений во Вьетнаме
DOI:
10.21668/health.risk/2020.3
DOI:
10.21668/health.risk
DOI:
10.21668/health.risk/2020.3.17
Language:
Unknown
Publisher:
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Publication Date:
2020
detail.hit.zdb_id:
2951489-7
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