In:
Proceedings, annual meeting, Electron Microscopy Society of America, Cambridge University Press (CUP), Vol. 48, No. 2 ( 1990-08-12), p. 68-69
Abstract:
The application of standards, with a known externally determined element concentration, for the determination of unknown concentrations in cell organelles and tissue is a well known practice in X-ray microanalysis. The conditions to be met for a good standard have been formulated earlier. Pure element standards and standards made from PVP-films have been proposed for Electron Energy loss analysis. In this presentation we investigate the use for EELS-analysis of the ion-exchange bead Chelex 100 -type of standards, which can be co-embedded with tissue and have been applied successfully for X-ray microanalysis. The ion-exchange characteristics, the methods of loading and the matrix composition have been described before. Such bio-standards, which can be loaded with a variety of cations, are stored as a dry powder and can be co-embedded with the tissue to be analyzed. In that way the standard is present in each ultrathin section, at (an assumed) equal thickness as the cells or tissue, containing the unknown concentration of that element.
Type of Medium:
Online Resource
ISSN:
0424-8201
,
2690-1315
DOI:
10.1017/S042482010013393X
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
1990
SSG:
11
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