In:
Magnetic Resonance in Medicine, Wiley, Vol. 59, No. 6 ( 2008-06), p. 1225-1231
Abstract:
Magnetic resonance imaging (MRI) is routinely used to obtain anatomical images that have greatly advanced biomedical research and clinical health care today, but the full potential of MRI in providing functional, physiological, and molecular information is only beginning to emerge. In this work, we sought to provide a gene expression marker for MRI based on bacterial magnetosomes, tiny magnets produced by naturally occurring magnetotactic bacteria. Specifically, magA , a gene in magnetotactic bacteria known to be involved with iron transport, is expressed in a commonly used human cell line, 293FT, resulting in the production of magnetic, iron‐oxide nanoparticles by these cells and leading to increased transverse relaxivity. MRI shows that these particles can be formed in vivo utilizing endogenous iron and can be used to visualize cells positive for magA . These results demonstrate that magA alone is sufficient to produce magnetic nanoparticles and that it is an appropriate candidate for an MRI reporter gene. Magn Reson Med 59:1225–1231, 2008. © 2008 Wiley‐Liss, Inc.
Type of Medium:
Online Resource
ISSN:
0740-3194
,
1522-2594
Language:
English
Publisher:
Wiley
Publication Date:
2008
detail.hit.zdb_id:
1493786-4
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