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  • 1
    In: Cancer Research, American Association for Cancer Research (AACR), Vol. 71, No. 8_Supplement ( 2011-04-15), p. 3111-3111
    Abstract: Alteration of organized breast epithelial ductal architecture accompanies neoplastic transformation. Obesity and chronic inflammation are associated with increased risk of postmenopausal breast cancer (BC). Further, omega 6 arachodonic acid (AA) significantly increases the secretion of pro-inflammatory cytokines by adipocytes compared to omega 3 eicosapentaenoic acid (EPA), therefore, we compared the effect of adipocytes and these two fatty acids on primary breast epithelial cell growth and formation of acini-like spheroid architecture. We used three dimensional (3D) MatrigelTM cultures of both basal and luminal primary breast cells and a novel 3D co-culture system with primary breast epithelial cells (basal/luminal) and adipocytes from lean or obese women. Effects of adipocytes as well as fatty acids on cellular architecture were assessed. Culture of basal or luminal epithelial cells with obese adipocytes/AA showed a significant increase in the irregularity of acini-like spheroid formation compared to normal spheroid formation in the absence of adipocytes or AA. Conditioned media transfer from lean/obese adipocytes into growing breast cell cultures showed remarkably different effects on LKB1/AMPK/mTOR metabolic signaling systems specifically in invasive MDA MB 321 cells. Obese adipocyte media decreased the activation of LKB1, AMPK, Acetyl-CoA carboxylase (ACC) and mTOR levels compared to lean adipocyte media. Further the expression of LKB1, pLKB1, pACC and mTOR were significantly higher in MDA MB 231 cells compared to less invasive MCF7 or epithelial MCF10A cells. Our data highlights the regulatory influence of adipocytes and inflammatory fatty acids on breast cellular architecture as well as metabolism and such alterations may underlie the effects of obesity on transformation of normal breast cells to BC cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 3111. doi:10.1158/1538-7445.AM2011-3111
    Type of Medium: Online Resource
    ISSN: 0008-5472 , 1538-7445
    RVK:
    RVK:
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2011
    detail.hit.zdb_id: 2036785-5
    detail.hit.zdb_id: 1432-1
    detail.hit.zdb_id: 410466-3
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  • 2
    Online Resource
    Online Resource
    Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences ; 2016
    In:  Acta Physica Sinica Vol. 65, No. 24 ( 2016), p. 245201-
    In: Acta Physica Sinica, Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences, Vol. 65, No. 24 ( 2016), p. 245201-
    Abstract: The structure of an electronegative plasma sheath in an oblique magnetic field is investigated. Moreover, the collisions between positive ions and neutral particles are taken into account. It is assumed that the system consists of hot electrons, hot negative ions, and cold positive ions. Also the negative ions and the electrons are assumed to be described by the Boltzmann distributions of their own temperatures, and the accelerated positive ions are treated by the continuity and momentum balance equations through the sheath region. In addition, it is assumed that the collision cross section has a power law dependence on the positive velocity. After theoretical derivation, an exact expression of sheath criterion is obtained. The numerical simulation results include the density distributions of the positive ions for different invariable ion Mach numbers satisfying Bohm criterion, and the comparison of net space charge distribution between variable and invariable ion Mach numbers. Furthermore, three kinds of charged particle densities, the net space charges, and the spatial electric potentials in the sheath are studied numerically for different collision parameters under the condition of the fixed ion Mach number. The results show that the ion Mach number has not only the lower limit but also the upper limit. The ion Mach number affects the sheath structure by influencing the distribution of the positive ion density, and different conclusions can be obtained because ion Mach number is adopted as variable or invariable value when discussing the effects of the other variables which can result in a variety of the ion Mach numbers on the sheath formation. The reason is that the actual sheath structure modification brought on by the variation of a parameter can be divided into two parts. One is the sheath formation change caused directly by the variation of the parameter, and the other is the sheath formation change caused by the Bohm criterion modification which the variation of the parameter results in. Therefore, an identical ion Mach number should be adopted when studying the direct effects of a parameter variety on plasma sheath structure. In addition, it is concluded that the collisions between positive ions and neutral particles make positive ion density curve higher and electron density curve lower than the case without collisions. Negative ion density does not change significantly no matter whether there exists collision. Besides, there is a peak in the profile of the net space charge while in the presence of ion-neutral collision, and the net space charge peak moves toward the sheath edge. The spatial potential increases and the sheath thickness decreases on account of the presence of the collisions between ions and neutral particles.
    Type of Medium: Online Resource
    ISSN: 1000-3290 , 1000-3290
    Language: Unknown
    Publisher: Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences
    Publication Date: 2016
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  • 3
    Online Resource
    Online Resource
    Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences ; 2012
    In:  Acta Physica Sinica Vol. 61, No. 3 ( 2012), p. 035201-
    In: Acta Physica Sinica, Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences, Vol. 61, No. 3 ( 2012), p. 035201-
    Abstract: The Bohm criterion for an electronegative magnetized plasma sheath is investigated with a fluid model. It is shown that the existence of negative ions has an effect on the Bohm criterion. And the numerical value of the ion Mach number depends on the magnetic field and the incidence status of the ions.
    Type of Medium: Online Resource
    ISSN: 1000-3290 , 1000-3290
    Language: Unknown
    Publisher: Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences
    Publication Date: 2012
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  • 4
    In: Food & Function, Royal Society of Chemistry (RSC), Vol. 14, No. 2 ( 2023), p. 911-920
    Abstract: Background : Epidemiological evidence regarding the relationship between dietary antioxidant vitamin intake and ovarian cancer (OC) survival is not clear. Herein, we aimed to first evaluate this topic in a prospective cohort study in China. Methods : The present study included participants from the Ovarian Cancer Follow-Up Study, which was a hospital-based prospective cohort study including OC patients who were aged 18 to 79 years during 2015–2020. The information on the intake of antioxidant vitamins, consisting of vitamin A, retinol, α-carotene, β-carotene, vitamin C, and vitamin E, and other diet information was obtained through a 111-item food frequency questionnaire. Deaths were recorded until March 31, 2021. Hazard ratios (HRs) and 95% confidence intervals (CIs) for overall survival were evaluated using Cox proportional hazards models. Results : There were 130 (18.49%) deaths among 703 OC patients during a median 37.19 months follow-up. In the multivariable-adjusted model, the highest tertile of dietary vitamin C (HR = 0.43, 95% CI = 0.25–0.75, P for trend 〈 0.05) and β-carotene intake (HR = 0.52, 95% CI = 0.31–0.87, P for trend 〈 0.05) was inversely associated with the overall survival of OC when compared with the lowest tertile group. Retinol, vitamin A, vitamin E, and α-carotene consumption showed no association with OC survival. Of note is that the multiplicative interaction was identified between vitamin C intake and residual lesions in OC survival ( P for interaction 〈 0.05). Conclusion : Our findings indicate that pre-diagnostic higher vitamin C and β-carotene intake was associated with improved OC survival.
    Type of Medium: Online Resource
    ISSN: 2042-6496 , 2042-650X
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2023
    detail.hit.zdb_id: 2578152-2
    SSG: 21
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  • 5
    Online Resource
    Online Resource
    Frontiers Media SA ; 2022
    In:  Frontiers in Cardiovascular Medicine Vol. 9 ( 2022-8-9)
    In: Frontiers in Cardiovascular Medicine, Frontiers Media SA, Vol. 9 ( 2022-8-9)
    Abstract: The purpose of this study was to evaluate the prognosis of patients with anomalous left coronary artery originating from pulmonary artery with varying cardiac function after surgical correction. Methods This was a single-center retrospective cohort study including 51 patients with anomalous left coronary artery originating from pulmonary artery, all of whom underwent surgery at our center. Results All 5 deaths occurred in the pre-operative low cardiac function group ( n = 39). After corrected by body surface area, parameters such as left coronary artery, right coronary artery, left atrial diameter, left ventricular end-diastolic diameter, left ventricular end-systolic diameter, and main pulmonary artery diameter, were lower in patients in the normal cardiac function group than in the low cardiac function group. The rate of collateral circulation formation was higher in the normal cardiac function group. The proportion of changes of T wave was higher in the low cardiac function group ( P = 0.005), and the duration of vasoactive drugs (dopamine, milrinone, epinephrine, nitroglycerin.) was longer in the low cardiac function group. Left ventricular end-diastolic diameter, left ventricular end-systolic diameter, main pulmonary artery diameter, and left atrial diameter were smaller than those pre-operatively ( P & lt; 0.05). Left ventricular ejection fraction was higher than that pre-operatively ( P = 0.003). The degree of mitral regurgitation in the low cardiac function group was reduced post-operatively ( P & lt; 0.001). Conclusion There was a significant difference between the pre-operative baseline data of the low cardiac function group and the normal cardiac function group. After surgical repair, cardiac function gradually returned to normal in the low cardiac function group. The low cardiac function group required vasoactive drugs for a longer period of time. The left ventricular end-diastolic diameter, left ventricular end-systolic diameter, left atrial diameter, and main pulmonary artery diameter decreased and gradually returned to normal after surgery. The degree of mitral regurgitation in the low cardiac function group was reduced after surgery.
    Type of Medium: Online Resource
    ISSN: 2297-055X
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2781496-8
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  • 6
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2020
    In:  Maternal-Fetal Medicine Vol. 2, No. 2 ( 2020-03-13), p. 68-71
    In: Maternal-Fetal Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 2, No. 2 ( 2020-03-13), p. 68-71
    Abstract: Since the outbreak of the novel coronavirus in Wuhan, China, as obstetricians, we also face great challenges. We need to identify pregnant patients with 2019 coronavirus disease infection timely, and give them appropriate treatment in order to obtain a good maternal and infant prognosis. Here, we would like to share a case and provide some suggestions on how to screen, diagnose and treat pregnant women with 2019 coronavirus disease infection during the outbreak.
    Type of Medium: Online Resource
    ISSN: 2641-5895
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2020
    detail.hit.zdb_id: 3026863-1
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  • 7
    Online Resource
    Online Resource
    IOP Publishing ; 2011
    In:  Chinese Physics Letters Vol. 28, No. 12 ( 2011-12), p. 125201-
    In: Chinese Physics Letters, IOP Publishing, Vol. 28, No. 12 ( 2011-12), p. 125201-
    Type of Medium: Online Resource
    ISSN: 0256-307X , 1741-3540
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 2011
    detail.hit.zdb_id: 2040565-0
    SSG: 6,25
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  • 8
    In: Circulation, Ovid Technologies (Wolters Kluwer Health), Vol. 145, No. 24 ( 2022-06-14), p. 1784-1798
    Abstract: IDO1 (indoleamine 2,3-dioxygenase 1) is the rate-limiting enzyme for tryptophan metabolism. IDO1 malfunction is involved in the pathogenesis of atherosclerosis. Vascular smooth muscle cells (VSMCs) with an osteogenic phenotype promote calcification and features of plaque instability. However, it remains unclear whether aberrant IDO1-regulated tryptophan metabolism causes VSMCs osteogenic reprogramming and calcification. Methods: We generated global Apoe ( apolipoprotein E ) and Ido1 double knockout mice, and Apoe knockout mice with specific deletion of IDO1 in VSMCs or macrophages. Arterial intimal calcification was evaluated by a Western diet–induced atherosclerotic calcification model. Results: Global deficiency of IDO1 boosted calcific lesion formation without sex bias in vivo. Conditional IDO1 loss of function in VSMCs rather than macrophages promoted calcific lesion development and the abundance of RUNX2 (runt-related transcription factor 2). In contrast, administration of kynurenine via intraperitoneal injection markedly delayed the progression of intimal calcification in parallel with decreased RUNX2 expression in both Apoe −/− and Apoe −/− Ido1 −/− mice. We found that IDO1 deletion restrained RUNX2 from proteasomal degradation, which resulted in enhanced osteogenic reprogramming of VSMCs. Kynurenine administration downregulated RUNX2 in an aryl hydrocarbon receptor–dependent manner. Kynurenine acted as the endogenous ligand of aryl hydrocarbon receptor, controlled resultant interactions between cullin 4B and aryl hydrocarbon receptor to form an E3 ubiquitin ligase that bound with RUNX2, and subsequently promoted ubiquitin-mediated instability of RUNX2 in VSMCs. Serum samples from patients with coronary artery calcification had impaired IDO1 activity and decreased kynurenine catabolites compared with those without calcification. Conclusions: Kynurenine, an IDO1-mediated tryptophan metabolism main product, promotes RUNX2 ubiquitination and subsequently leads to its proteasomal degradation via an aryl hydrocarbon receptor–dependent nongenomic pathway. Insufficient kynurenine exerts the deleterious role of IDO1 ablation in promoting RUNX2-mediated VSMCs osteogenic reprogramming and calcification in vivo.
    Type of Medium: Online Resource
    ISSN: 0009-7322 , 1524-4539
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2022
    detail.hit.zdb_id: 1466401-X
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  • 9
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2022
    In:  Nature Communications Vol. 13, No. 1 ( 2022-12-13)
    In: Nature Communications, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2022-12-13)
    Abstract: Consuming fish oils (FO) is linked to reduced risk of cardiovascular disease in certain populations. However, FO failed to exhibit therapeutic effects in some patients with cardiovascular disease. This study aimed to determine the possible reasons for the inconsistent effects of FO. AMP-activated protein kinase (AMPK) α2 is an important energy metabolic sensor, which was reported to involve in FO mediated regulation of lipid and glucose metabolism. In an in vivo study, FO administration significantly reduced the aortic lesions and inflammation in the Ldlr −/− mouse model of atherosclerosis, but not in Ldlr −/− / Prkaa2 −/− and Ldlr −/− / Prkaa2 −/−Sm22Cre mice. Mechanistically, inactivation of AMPKα2 increased the SUMOylation of the fatty acid receptor GPR120 to block FO-induced internalization and binding to β-arrestin. In contrast, activation of AMPKα2 can phosphorylate the C-MYC at Serine 67 to inhibit its trans-localization into the nuclei and transcription of SUMO-conjugating E2 enzyme UBC9 and SUMO2/3 in vascular smooth muscle cells (VSMCs), which result in GPR120 SUMOylation. In human arteries, AMPKα2 levels were inversely correlated with UBC9 expression. In a cohort of patients with atherosclerosis, FO concentrations did not correlate with atherosclerotic severity, however, in a subgroup analysis a negative correlation between FO concentrations and atherosclerotic severity was found in patients with higher AMPKα2 levels. These data indicate that AMPKα2 is required for the anti-inflammatory and anti-atherosclerotic effects of FO.
    Type of Medium: Online Resource
    ISSN: 2041-1723
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2553671-0
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  • 10
    In: Signal Transduction and Targeted Therapy, Springer Science and Business Media LLC, Vol. 8, No. 1 ( 2023-01-21)
    Abstract: Urinary stone is conceptualized as a chronic metabolic disorder punctuated by symptomatic stone events. It has been shown that the occurrence of calcium oxalate monohydrate (COM) during stone formation is regulated by crystal growth modifiers. Although crystallization inhibitors have been recognized as a therapeutic modality for decades, limited progress has been made in the discovery of effective modifiers to intervene with stone disease. In this study, we have used metabolomics technologies, a powerful approach to identify biomarkers by screening the urine components of the dynamic progression in a bladder stone model. By in-depth mining and analysis of metabolomics data, we have screened five differential metabolites. Through density functional theory studies and bulk crystallization, we found that three of them (salicyluric, gentisic acid and succinate) could effectively inhibit nucleation in vitro. We thereby assessed the impact of the inhibitors with an EG-induced rat model for kidney stones. Notably, succinate, a key player in the tricarboxylic acid cycle, could decrease kidney calcium deposition and injury in the model. Transcriptomic analysis further showed that the protective effect of succinate was mainly through anti-inflammation, inhibition of cell adhesion and osteogenic differentiation. These findings indicated that succinate may provide a new therapeutic option for urinary stones.
    Type of Medium: Online Resource
    ISSN: 2059-3635
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2886872-9
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