In:
Clinical and Experimental Pharmacology and Physiology, Wiley, Vol. 33, No. 8 ( 2006-08), p. 696-701
Abstract:
Mesenchymal stem cell (MSC) therapy is drawing increasing attention in cardiology. However, the effect of aspirin, an assistant medication used extensively in the treatment of cardiovascular diseases, on MSC is not clear. In the present study, we investigated the effect of aspirin on the growth of MSC in vitro and the underlying mechanism of its action. The 3‐(4,5‐dimethyl‐2 thiazoyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay revealed that 1, 5 and 10 mmol/L aspirin inhibited the growth of MSC by 18, 37 and 62%, respectively. DNA synthesis of MSC was inhibited by 25, 57 and 90% followoing treatment with 1, 5 and 10 mmol/L aspirin, respectively, as determined by the tritiated thymidine incorporation assay. No cytotoxicity was observed based on Trypan blue dye exclusion and cell morphological observations. Western blot analysis demonstrated that the protein level of phosphorylated β‐catenin increased, whereas that of cyclin D1 decreased, after treatment of MSC with aspirin. Cell cycle analysis showed that aspirin failed to significantly alter the proportion of cells in different stages of the cell cycle. These observations indicate that aspirin inhibits MSC proliferation and that the downregulation of the wnt/β‐catenin signal pathway may be involved in the growth inhibition of MSC by aspirin.
Type of Medium:
Online Resource
ISSN:
0305-1870
,
1440-1681
DOI:
10.1111/cep.2006.33.issue-8
DOI:
10.1111/j.1440-1681.2006.04432.x
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2020033-X
SSG:
15,3
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