In:
PeerJ, PeerJ, Vol. 10 ( 2022-05-31), p. e13375-
Abstract:
Herein, we aimed to present evidence that Ferulic acid (FA), a phenolic acid, can alleviate high glucose (HG)-induced retinal pigment epithelium (RPE) cell apoptosis and protect retina in db/db mice. Methods ARPE-19 cells (a human RPE cell line) were divided into four groups: control group; HG group (30 mmol/L glucose); HG+FA group (30 mmol/L glucose and 10 mmol/L FA). Cell viability and apoptosis were detected using CCK-8 and Annexin-5 staining, respectively. Apoptosis-related markers including P53, BAX and Bcl2 were examined by RT-qPCR, western blot and immunohistochemistry. Totally, 30 male db/db mice were randomly divided into db/db group (5 ml/kg saline) and FA group (0.05 g/kg FA). After treatment for 2 months, retinal samples were subjected to hematoxylin and eosin (H & E) and Masson staining. Moreover, immunofluorescence was used to detect apoptosis-related markers. Blood samples were collected for measuring cholesterol, triglyceride (TG), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) levels. Results FA treatment markedly increased cell viability and suppressed cell apoptosis of ARPE-19 cells compared to the HG-exposed group. Furthermore, FA ameliorated the abnormal expression levels of P53, BAX and Bcl2 in HG-induced ARPE-19 cells. In animal models, FA attenuated pathological changes in the retina tissues of diabetic mice. Consistent with in vitro models, FA significantly ameliorated the expression of apoptosis-related markers in retina tissues. Biochemical test results showed that FA reduced hyperlipidemia in diabetic mice. Conclusion Our findings suggest that FA alleviates HG-induced apoptosis in RPE cells and protects retina in db/db mice, which can be associated with P53 and BAX inactivation and Bcl2 activation.
Type of Medium:
Online Resource
ISSN:
2167-8359
DOI:
10.7717/peerj.13375/fig-1
DOI:
10.7717/peerj.13375/fig-2
DOI:
10.7717/peerj.13375/fig-3
DOI:
10.7717/peerj.13375/fig-4
DOI:
10.7717/peerj.13375/fig-5
DOI:
10.7717/peerj.13375/fig-6
DOI:
10.7717/peerj.13375/fig-7
DOI:
10.7717/peerj.13375/table-1
DOI:
10.7717/peerj.13375/supp-1
Language:
English
Publisher:
PeerJ
Publication Date:
2022
detail.hit.zdb_id:
2703241-3
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