In:
Genome, Canadian Science Publishing, Vol. 60, No. 8 ( 2017-08), p. 657-664
Abstract:
Single-strand oligonucleotides (SSONs hereafter) as probes are becoming a powerful method of chromosome painting in many species. In this study, nine SSONs ((ACT) 10 , (ACT) 19 , Knob-1, Knob-2, Knob-3, CentC69-1, MR68-3, K10-72-1, and TR1-357-2) were developed and used for chromosome identification in 16 maize (Zea mays L., 2n = 20) inbred lines and hybrids by non-denaturing fluorescence in situ hybridization (ND-FISH). Each SSON produced clear signals on 2–10 chromosomes of inbred lines B73 and Mo17. A multiplex probe set containing four SSONs ((ACT) 10 , Knob-2, CentC69-1, and MR68-3) clearly characterized all maize chromosomes in the 16 lines by a single round of ND-FISH and revealed genetic variation at a chromosome level. For example, unique signals on chromosome 6 clearly distinguished all 16 genotypes. The SSONs and multiplex probe developed in this research will facilitate genotype identification and chromosome research in maize.
Type of Medium:
Online Resource
ISSN:
0831-2796
,
1480-3321
DOI:
10.1139/gen-2016-0167
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
2017
detail.hit.zdb_id:
2020635-5
SSG:
12
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