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  • 1
    Online Resource
    Online Resource
    Clinical relevance of the in situ assay for HBV DNA: a cross-sectional study in patients with chronic hepatitis B
    BMJ ; 2020
    In:  Journal of Clinical Pathology Vol. 73, No. 12 ( 2020-12), p. 813-818
    Details
    In: Journal of Clinical Pathology, BMJ, Vol. 73, No. 12 ( 2020-12), p. 813-818
    Abstract: The visualisation of HBV DNA in liver sections of patients with chronic hepatitis B (CHB) in our previous report uncovered a mosaic distribution of viral antigens and nucleic acids. Here we aim to further explore the clinical utility of the in situ hybridisation (ISH) assay for HBV DNA. Method ISH of HBV DNA along with immunohistochemistry (IHC) of HBsAg, HBcAg and routine histopathology analysis was performed in 313 treatment-naive patients with CHB. Serum HBcrAg and HBcAb titre were also measured in addition to basic biochemical and virological parameters. Results The ISH of HBV DNA, HBsAg and HBcAg showed 95.2%, 97.1% and 42.8% positive rate, respectively. The staining pattern of HBV DNA differs significantly with that of HBsAg. Intrahepatic HBV DNA exhibited high-level of correlations with viral load, HBcrAg and HBsAg titre. In HBeAg-negative patients, higher intrahepatic HBV DNA is associated with histological evidence of liver inflammation and fibrosis, whereas no such trend was observed in HBeAg-positive patients. Finally, a triple staining protocol that combined the detection of HBV DNA, HBsAg and collagen fibre was developed to enable better evaluation of viral replication and antigen expression in the context of disease progression. Conclusions The ISH assay for HBV DNA reflects the vigour of intrahepatic viral replication. It is complementary to the routine IHC assay for viral antigens and also related to the histopathological progression of liver diseases. The application of the HBV DNA ISH assay may help a better evaluation of virological and pathological condition of patients with CHB.
    Type of Medium: Online Resource
    ISSN: 0021-9746 , 1472-4146
    URL: Article
    DOI: 10.1136/jclinpath-2020-206440
    RVK:
    XA 10000
    Language: English
    Publisher: BMJ
    Publication Date: 2020
    detail.hit.zdb_id: 2028928-5
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  • 2
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    DAF ‐16 is involved in colonic metabolites of ferulic acid‐promoted longevity and stress resistance of Caenorhabditis elegans
    Wiley ; 2022
    In:  Journal of the Science of Food and Agriculture Vol. 102, No. 15 ( 2022-12), p. 7017-7029
    Details
    In: Journal of the Science of Food and Agriculture, Wiley, Vol. 102, No. 15 ( 2022-12), p. 7017-7029
    Abstract: Ferulic acid (FA) is a dietary polyphenol widely found in plant tissues. It has long been considered to have health‐promoting qualities. However, the biological properties of dietary polyphenols depend largely on their absorption during digestion, and the effects of their intestinal metabolites on human health have attracted the interest of researchers. This study evaluated the effects of three main colonic metabolites of FA – 3‐(3,4‐dihydroxyphenyl)propionic acid (3,4diOHPPA), 3‐(3‐hydroxyphenyl)propionic acid (3OHPPA) and 3‐phenylpropionic acid (3PPA) – on longevity and stress resistance in Caenorhabditis elegans . RESULTS Our results showed that 3,4diOHPPA, 3OHPPA and 3PPA extended the lifespan under normal conditions in C. elegans whereas FA did not. High doses of 3,4diOHPPA (0.5 mmol L −1 ), 3OHPPA (2.5 mmol L −1 ) and 3PPA (2.5 mmol L −1 ) prolonged the mean lifespan by 11.2%, 13.0% and 10.6%, respectively. Moreover, 3,4diOHPPA, 3OHPPA and 3PPA treatments promoted stress tolerance against heat, UV irradiation and paraquat. Furthermore, three metabolites ameliorated physical functions, including reactive oxygen species and malondialdehyde levels, motility and pharyngeal pumping rate. The anti‐aging activities mediated by 3,4diOHPPA, 3OHPPA and 3PPA depend on the HSF‐1 and JNK‐1 linked insulin/IGF‐1 signaling pathway, which converge onto DAF‐16. CONCLUSION The current findings suggest that colonic metabolites of FA have the potential for use as anti‐aging bioactivate compounds. © 2022 Society of Chemical Industry.
    Type of Medium: Online Resource
    ISSN: 0022-5142 , 1097-0010
    URL: Issue
    URL: Article
    DOI: 10.1002/jsfa.v102.15
    DOI: 10.1002/jsfa.12063
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2001807-1
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  • 3
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    Evaluation of the in situ assay for HBV DNA : An observational real-world study in chronic hepatitis B
    Ovid Technologies (Wolters Kluwer Health) ; 2021
    In:  Medicine Vol. 100, No. 37 ( 2021-09-17), p. e27220-
    Details
    In: Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 100, No. 37 ( 2021-09-17), p. e27220-
    Abstract: The visualization of intrahepatic hepatitis B virus (HBV) DNA by in situ hybridization (ISH) has uncovered some interesting aspects of HBV life cycle at the single-cell level. In the current study, we intend to evaluate the reliability and robustness of this assay in the real-world clinical scenario and its relationship with currently available clinical biomarkers in chronic hepatitis B (CHB) patients. In this cross-sectional study, 94 CHB patients and 10 patients with non-HBV related liver diseases were enrolled. Liver biopsies and routine histopathology analysis were performed. Intrahepatic HBV DNA and viral antigens (HBsAg and HBcAg) were detected by ISH and immunohistochemistry (IHC), respectively. The basic biochemical and virological parameters such as alanine transaminase, serum HBV DNA, and serum HBsAg were measured. The HBV DNA-ISH assay showed 55.8% (53/94 cases) positive rate in CHB patients, no false positive was found in non-HBV related hepatitis. The IHC of HBsAg and HBcAg showed a positive rate of 94.7% (89/94 cases) and 19.5% (17/87 cases), respectively. Quantification of HBV DNA-ISH signal showed a significant correlation with serum HBV DNA ( r s  = 0.6223, P   〈  .0001). In addition, the staining pattern of HBV DNA in situ in the context of collagen deposition informed the histopathological progression of chronic liver disease. The application of this ISH assay in evaluating intrahepatic viral replication in real-world CHB patients showed favorable performance. It can be a complementation to conventional liver histopathology examination and IHC detection of viral antigens. This methodology provides an intuitive assessment of virological and pathological state of CHB patients, and further supports clinical diagnosis and management.
    Type of Medium: Online Resource
    ISSN: 0025-7974 , 1536-5964
    URL: Article
    DOI: 10.1097/MD.0000000000027220
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2021
    detail.hit.zdb_id: 2049818-4
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  • 4
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    NCAPG Promotes the Proliferation of Renal Clear Cell Carcinoma via Mediating with CDK1
    Hindawi Limited ; 2022
    In:  Disease Markers Vol. 2022 ( 2022-5-13), p. 1-12
    Details
    In: Disease Markers, Hindawi Limited, Vol. 2022 ( 2022-5-13), p. 1-12
    Abstract: Objective. Currently, lots of scholars have proved that the expression of NCAPG is associated with the prognosis of several cancers, while the relationship between NCAPG and renal clear cell carcinoma remains unclear, so the main aim of this research is to explore the effects of NCAPG on the progression of renal clear cell carcinoma. Methods. We observed the differential expression of NCAPG in several cancers from GEPIA online database, and the expression of NCAPG in renal clear cell carcinoma and normal tissue was compared and further verified by IHC assay. CCK-8 assay and clone formation experiment were conducted to observe the change of NCAPG on the proliferation. GraphPad was used for data analysis, and t -test and χ 2 analysis were used to analyze the correlation between NCAPG/CDK1 and renal clear cell carcinoma. Results. NCAPG was upregulated in renal clear cell carcinoma compared with the normal tissue, and the expression of NCAPG was associated with the clinical prognosis of pancreatic cancer especially with tumor size ( P = 0.010 ). Knockdown NCAPG could restrain the proliferation of renal clear cell carcinoma. CDK1 was found to be tightly related with NCAPG, and the expression of CDK1 was also associated with the prognosis. Conclusions. NCAPG was upregulated in renal clear cell carcinoma, which was related with tumor size and overall survival. NCAPG might promote the proliferation of renal clear cell carcinoma via mediating CDK1. NCAPG/CDK1 complex might provide a new treatment strategy for lots of patients with renal clear cell carcinoma.
    Type of Medium: Online Resource
    ISSN: 1875-8630 , 0278-0240
    URL: Article
    DOI: 10.1155/2022/6758595
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2033253-1
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  • 5
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    Mechanisms of the regulation of ester balance between oxidation and esterification in aged Baijiu
    Springer Science and Business Media LLC ; 2020
    In:  Scientific Reports Vol. 10, No. 1 ( 2020-10-13)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2020-10-13)
    Abstract: The influence of oxidation and esterification on the ester balance of aged Baijiu and methodology for the rational design of liquor flavors to optimize the practice of Baijiu production are not completely understood. We investigated the influence of ester balance on the flavor compounds of aged Baijiu by conducting constituent analysis of Baijiu from different aging times (0, 1, 2, 3, 4, 5, and 10 years). The changes of the main flavor compounds in the aging process were determined, and the correlations among different chemical reactions, such as oxidation, hydrolysis, and esterification, were systematically expounded. Furthermore, cluster analysis of the heat map indicated significant differences between aged Baijiu and new Baijiu and recommended a suitable aging time of 2–3 years.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-020-74423-z
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2615211-3
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  • 6
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    Online Resource
    Nobiletin Delays Aging and Enhances Stress Resistance of Caenorhabditis elegans
    MDPI AG ; 2020
    In:  International Journal of Molecular Sciences Vol. 21, No. 1 ( 2020-01-04), p. 341-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 21, No. 1 ( 2020-01-04), p. 341-
    Abstract: Nobiletin (NOB), one of polymethoxyflavone existing in citrus fruits, has been reported to exhibit a multitude of biological properties, including anti-inflammation, anti-oxidation, anti-atherosclerosis, neuroprotection, and anti-tumor activity. However, little is known about the anti-aging effect of NOB. The objective of this study was to determine the effects of NOB on lifespan, stress resistance, and its associated gene expression. Using Caenorhabditis elegans, an in vivo nematode model, we found that NOB remarkably extended the lifespan; slowed aging-related functional declines; and increased the resistance against various stressors, including heat shock and ultraviolet radiation. Also, NOB reduced the effects of paraquat stressor on nematodes and scavenged reactive oxygen species (ROS). Furthermore, gene expression revealed that NOB upregulated the expression of sod-3, hsp-16.2, gst-4, skn-1, sek-1, and sir-2.1, which was suggested that anti-aging activity of NOB was mediated most likely by activation of the target genes of the transcription factors including dauer formation (DAF)-16, heat-shock transcription factor (HSF)-1, and skinhead (SKN)-1. In summary, NOB has potential application in extension of lifespan, and its associated healthspan and stress resistances.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms21010341
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 7
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    Phytochemical Profiles, Antioxidant Activity and Antiproliferative Mechanism of Rhodiola rosea L. Phenolic Extract
    MDPI AG ; 2022
    In:  Nutrients Vol. 14, No. 17 ( 2022-08-31), p. 3602-
    Details
    In: Nutrients, MDPI AG, Vol. 14, No. 17 ( 2022-08-31), p. 3602-
    Abstract: The phenolic profiles, antioxidant activity, antiproliferative property and the underlying molecular mechanisms of cell apoptosis of Rhodiola rosea free phenolic (RFE) were analyzed in this work. Overall, Rhodiola rosea rhizome phenolic extract (RE) contained Rhodiola rosea rhizome free phenolic extract (RFE) and Rhodiola rosea rhizome bound phenolic extract (RBE). Compared with RBE, RFE contained higher phenolic contents and possessed stronger antioxidant activity. High-performance liquid chromatography (HPLC) results demonstrated that the main phenolics of were epigallocatechin (EGC), epigallocatechin gallate (EGCG), gallic acid (GA) and catechin. Gas chromatography–mass spectrometry (GC-MS) analysis found that Rhodiola rosea L. was rich in volatile phytochemicals. In addition, many types of vitamin E and a few kinds of carotenoids were found in Rhodiola rosea. In addition, the main compounds in RFE (GA, EGC, EGCG) and RFE all exhibited excellent antiproliferative activity, indicating the antiproliferative activity of RFE was partly attributed to the synergy effects of the main compounds. Further study confirmed that RFE could block 16.99% of HepG2 cells at S phase and induce 20.32% programmed cell death compared with the control group. Specifically, RFE dose-dependently induced cell apoptosis and cell cycle arrest via modulating the p53 signaling pathway including up-regulation of the expression of p53 and Bax while down-regulation of the Bcl-2, cyclin D1 and CDK4 levels. Therefore, RFE exhibited the potential of being developed as an auxiliary antioxidant and a therapeutic agent for cancer.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    URL: Article
    DOI: 10.3390/nu14173602
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518386-2
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  • 8
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    Phenolics content, antioxidant and antiproliferative activities of dehulled highland barley (Hordeum vulgare L.)
    Elsevier BV ; 2015
    In:  Journal of Functional Foods Vol. 19 ( 2015-12), p. 439-450
    Details
    In: Journal of Functional Foods, Elsevier BV, Vol. 19 ( 2015-12), p. 439-450
    Type of Medium: Online Resource
    ISSN: 1756-4646
    URL: Article
    DOI: 10.1016/j.jff.2015.09.053
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2015
    detail.hit.zdb_id: 2467241-5
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  • 9
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    The use of an enzymatic extraction procedure for the enhancement of highland barley ( Hordeum vulgare L.) phenolic and antioxidant compounds
    Wiley ; 2016
    In:  International Journal of Food Science & Technology Vol. 51, No. 8 ( 2016-08), p. 1916-1924
    Details
    In: International Journal of Food Science & Technology, Wiley, Vol. 51, No. 8 ( 2016-08), p. 1916-1924
    Abstract: A multistep enzymatic extraction method was compared with a conventional chemical extraction process to evaluate the phenolic content and antioxidant activity of highland barley (Z2, Zangqing 25; CHQK , Changheiqingke ). The main phenolic compound extracted was (+)‐catechin, followed by ferulic acid, p ‐coumaric acid, caffeic acid and chlorogenic acid. The multi‐enzymatic digestion yielded a higher retrieval of (+)‐catechin compared to the conventional chemical extraction procedure ( P  〈   0.05). Compounds obtained from the multi‐enzymatic digestion process exhibited significantly higher ( P  〈   0.05) antioxidant activities determined by oxygen radical absorbance capacity ( ORAC ) and cellular antioxidant activity ( CAA ) when compared to chemical extracts. These results suggest that highland barley subjected to in vitro multi‐enzymatic digestion exhibits a higher phenolic content and antioxidant activity than the chemical extraction, and this multi‐enzymatic digestion coupled with the CAA assay may be a valuable tool to evaluate the antioxidant potential of wholegrains and fruits, as well as vegetables.
    Type of Medium: Online Resource
    ISSN: 0950-5423 , 1365-2621
    URL: Issue
    URL: Article
    DOI: 10.1111/ijfs.2016.51.issue-8
    DOI: 10.1111/ijfs.13165
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2016518-3
    detail.hit.zdb_id: 2401430-8
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  • 10
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    Altered microbial resource limitation regulates soil organic carbon sequestration based on ecoenzyme stoichiometry under long‐term tillage systems
    Wiley ; 2022
    In:  Land Degradation & Development Vol. 33, No. 15 ( 2022-09), p. 2795-2808
    Details
    In: Land Degradation & Development, Wiley, Vol. 33, No. 15 ( 2022-09), p. 2795-2808
    Abstract: Soil microbial metabolism is critically important for regulating soil carbon (C) sequestration. However, how soil organic C (SOC) stock responds to the changes in microbial resource limitation and microbial C use efficiency (CUE) under long‐term tillage system remains uncertain. Soil samples were randomly collected from a long‐term (19 years) tillage experiment growing winter wheat with three treatments [i.e., moldboard‐plough without straw retention (MPN), no‐tillage with straw retention (NTS), and subsoiling with straw retention (SSS)] and an adjacent natural grassland (GRL) in 2018. We analyzed microbial resource limitation and CUE based on ecoenzyme stoichiometry. Results revealed that the reduction of soil bulk density and total dissolved nitrogen (N) resulted in an increased vector angle. Higher total dissolved N and lower vector length jointly led to higher CUE under long‐term conservation tillage practices (i.e., NTS and SSS). Notably, the higher CUE and soil available phosphorus as well as the lower N limitation both directly contributed explaining the increase in SOC stock under conservation tillage practices. Overall, long‐term conservation tillage practices could alleviate microbial resource limitation by enhancing available nutrients to promote SOC sequestration. Our study provides new knowledge to enhance the understanding of SOC stock regulated by microbial resource limitations and microbial C use efficiency.
    Type of Medium: Online Resource
    ISSN: 1085-3278 , 1099-145X
    URL: Issue
    URL: Article
    DOI: 10.1002/ldr.v33.15
    DOI: 10.1002/ldr.4318
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2021787-0
    detail.hit.zdb_id: 1319202-4
    SSG: 14
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