In:
Analytical Methods, Royal Society of Chemistry (RSC), Vol. 14, No. 22 ( 2022), p. 2168-2178
Abstract:
Doxepin, a tricyclic antidepressant (TCA), is widely used in the treatment of depressive disorder and anxiety. There are some liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods that have been reported for detecting doxepin, but inadequacies in recovery and cumbersome sample preparation obstruct the pharmacokinetics study. Therefore, we aimed to develop and validate a rapid sample preparation method based on solid-phase extraction (SPE) for the precise quantification of doxepin and its metabolites. Chromatography separation was performed on a Waters ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) and a mobile phase consisting of 70% of mobile phase A (0.1% formic acid and 10 mM ammonium formate) and 30% mobile phase B (0.1% formic acid in acetonitrile) at a flow rate of 0.4 mL min −1 in the step gradient elution conditions. The lower limits of quantification for doxepin and N -nordoxepin were 4 pg mL −1 and 2 pg mL −1 , respectively. This method was validated with satisfactory results including good precision and accuracy. A rapid, sensitive, and specific LC-MS/MS method was developed and validated for the determination of doxepin in human plasma. This method could be applied for determining doxepin and N -nordoxepin concentrations in plasma that could be useful for bioequivalence study of 3 mg doxepin.
Type of Medium:
Online Resource
ISSN:
1759-9660
,
1759-9679
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2022
detail.hit.zdb_id:
2515210-5
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