In:
Food & Function, Royal Society of Chemistry (RSC)
Abstract:
Two novel Flammulina velutipes ( F. velutipes ) polysaccharides, FVPH1 and FVPH2, were isolated and purified after hot water extraction. The structural characterization revealed that the backbone of FVPH1 consisted mainly of →6)-α- d -Glc p (1→, →3,4)-α- d -Gal p (1→, →4)-α- l -Fuc p (1→, and →4)-β- d -Man p (1→, while the backbone of FVPH2 consisted of →3)-α- d -Gal p (1→, →3,4)-α- d -Man p (1→,→6)-α- d -Glc p (1→. The branches of FVPH1 contained →6)-α- d -Glc p (1→ and α- d -Glc p (1→ and the branches of FVPH2 consisted of →3)-α- d -Gal p (1→, →6)-α- d -Glc p (1→, and β- l -Fuc p (1→. FVPH2 exhibited significantly better immunostimulatory activity than FVPH1 ( P 〈 0.05), as evidenced by the increased expression of NO, IL-1β, IL-6, and TNF-α and pinocytic activity of RAW264.7 cells. As the most abundant structure in the polysaccharides of F. velutipes , the content of →6)-α- d -Glc p (1→ might play a crucial role in influencing the immunostimulatory activity of F. velutipes polysaccharides. The F. velutipes polysaccharide with a lower content of →6)-α- d -Glc p (1→ and a higher branching degree could significantly enhance the immunostimulatory activity of F. velutipes polysaccharides via activating the TLR-4/MyD88/NF-κB pathway more effectively.
Type of Medium:
Online Resource
ISSN:
2042-6496
,
2042-650X
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2024
detail.hit.zdb_id:
2578152-2
SSG:
21
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