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  • 1
    Online Resource
    Online Resource
    A PECTIN METHYLESTERASE gene at the maize Ga1 locus confers male function in unilateral cross-incompatibility
    Springer Science and Business Media LLC ; 2018
    In:  Nature Communications Vol. 9, No. 1 ( 2018-09-10)
    Details
    In: Nature Communications, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2018-09-10)
    Abstract: Unilateral cross-incompatibility (UCI) is a unidirectional inter/intra-population reproductive barrier when both parents are self-compatible. Maize Gametophyte factor1 ( Ga1 ) is an intraspecific UCI system and has been utilized in breeding. However, the mechanism underlying maize UCI specificity has remained mysterious for decades. Here, we report the cloning of ZmGa1P , a pollen-expressed PECTIN METHYLESTERASE ( PME ) gene at the Ga1 locus that can confer the male function in the maize UCI system. Homozygous transgenic plants expressing ZmGa1P in a ga1 background can fertilize Ga1-S plants and can be fertilized by pollen of ga1 plants. ZmGa1P protein is predominantly localized to the apex of growing pollen tubes and may interact with another pollen-specific PME protein, ZmPME10-1, to maintain the state of pectin methylesterification required for pollen tube growth in Ga1-S silks. Our study discloses a PME-mediated UCI mechanism and provides a tool to manipulate hybrid breeding.
    Type of Medium: Online Resource
    ISSN: 2041-1723
    URL: Article
    DOI: 10.1038/s41467-018-06139-8
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2018
    detail.hit.zdb_id: 2553671-0
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  • 2
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    Online Resource
    Transpositional behaviour of the Ds element in the Ac/Ds system in rice
    Springer Science and Business Media LLC ; 2007
    In:  Chinese Science Bulletin Vol. 52, No. 20 ( 2007-10), p. 2789-2796
    Details
    In: Chinese Science Bulletin, Springer Science and Business Media LLC, Vol. 52, No. 20 ( 2007-10), p. 2789-2796
    Type of Medium: Online Resource
    ISSN: 1001-6538 , 1861-9541
    URL: Article
    DOI: 10.1007/s11434-007-0415-6
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2007
    detail.hit.zdb_id: 2069521-4
    detail.hit.zdb_id: 2816140-3
    SSG: 11
    SSG: 6,25
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  • 3
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    Diagnostic performance of low-radiation-dose and low-contrast-dose (double low-dose) coronary CT angiography for coronary artery stenosis
    Ovid Technologies (Wolters Kluwer Health) ; 2018
    In:  Medicine Vol. 97, No. 34 ( 2018-08), p. e11798-
    Details
    In: Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 97, No. 34 ( 2018-08), p. e11798-
    Type of Medium: Online Resource
    ISSN: 0025-7974
    URL: Article
    DOI: 10.1097/MD.0000000000011798
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2018
    detail.hit.zdb_id: 2049818-4
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  • 4
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    Identification of the Potential Genes Regulating Seed Germination Speed in Maize
    MDPI AG ; 2022
    In:  Plants Vol. 11, No. 4 ( 2022-02-19), p. 556-
    Details
    In: Plants, MDPI AG, Vol. 11, No. 4 ( 2022-02-19), p. 556-
    Abstract: Seed germination is the crucial stage in plant life cycle. Rapid and uniform germination plays an essential role in plant development and grain yield improvement. However, the molecular mechanism underlying seed germination speed is largely unknown due to the complexity of the dynamic process and the difficulty in phenotyping. Here, we conducted a time-series comparative transcriptome study of two elite maize inbred lines, 72-3 and F9721, with striking difference in seed germination speed, and identified a major locus underlying maize germination speed through genome-wide association analysis (GWAS) of an F2 segregation population. Comparative transcriptome study identified 12 h after imbibition (HAI) as the critical stage responsible for the variation in germination speed. The differentially expressed genes (DEGs) between 72-3 and F9721 were mainly enriched in metabolic pathways, biosynthesis of secondary metabolites, oxidoreductase activity pathways, hormone signal transduction, and amino acid transporter activity pathways. GWAS revealed that germination speed was controlled by a major locus on chromosome 1 with the leading SNP as AX-91332814, explaining 10.63% of phenotypic variation. A total of 87 proposed protein-coding genes surrounding the locus were integrated with DEGs. Combined with evidence from the gene expression database and gene synteny with other model species, we finally anchored three genes as the likely candidates regulating germination speed in maize. This study provides clues for the further exploration of genes controlling the maize seed germination speed, thus facilitating breeding of rapid germinated elite lines through marker assistant selection.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    URL: Article
    DOI: 10.3390/plants11040556
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2704341-1
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  • 5
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    The improved assembly of 7DL chromosome provides insight into the structure and evolution of bread wheat
    Wiley ; 2020
    In:  Plant Biotechnology Journal Vol. 18, No. 3 ( 2020-03), p. 732-742
    Details
    In: Plant Biotechnology Journal, Wiley, Vol. 18, No. 3 ( 2020-03), p. 732-742
    Abstract: Wheat is one of the most important staple crops worldwide and also an excellent model species for crop evolution and polyploidization studies. The breakthrough of sequencing the bread wheat genome and progenitor genomes lays the foundation to decipher the complexity of wheat origin and evolutionary process as well as the genetic consequences of polyploidization. In this study, we sequenced 3286 BAC s from chromosome 7 DL of bread wheat cv. Chinese Spring and integrated the unmapped contigs from IWGSC v1 and available PacBio sequences to close gaps present in the 7 DL assembly. In total, 8043 out of 12 825 gaps, representing 3 491 264 bp, were closed. We then used the improved assembly of 7 DL to perform comparative genomic analysis of bread wheat (Ta7 DL ) and its D donor, Aegilops tauschii (At7 DL ), to identify domestication signatures. Results showed a strong syntenic relationship between Ta7 DL and At7 DL , although some small rearrangements were detected at the distal regions. A total of 53 genes appear to be lost genes during wheat polyploidization, with 23% (12 genes) as RGA (disease resistance gene analogue). Furthermore, 86 positively selected genes ( PSG s) were identified, considered to be domestication‐related candidates. Finally, overlapping of QTL s obtained from GWAS analysis and PSG s indicated that Traes CS 7D02G321000 may be one of the domestication genes involved in grain morphology. This study provides comparative information on the sequence, structure and organization between bread wheat and Ae. tauschii from the perspective of the 7 DL chromosome , which contribute to better understanding of the evolution of wheat, and supports wheat crop improvement.
    Type of Medium: Online Resource
    ISSN: 1467-7644 , 1467-7652
    URL: Issue
    URL: Article
    DOI: 10.1111/pbi.v18.3
    DOI: 10.1111/pbi.13240
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2136367-5
    SSG: 12
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  • 6
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    Research on Fine Chemical Process and Equipment Detection and Computer Monitoring Technology
    IOP Publishing ; 2021
    In:  Journal of Physics: Conference Series Vol. 1992, No. 2 ( 2021-08-01), p. 022152-
    Details
    In: Journal of Physics: Conference Series, IOP Publishing, Vol. 1992, No. 2 ( 2021-08-01), p. 022152-
    Abstract: The quality of fine chemical material production is a very important content in chemical production process. It is necessary to do a good job of process control related factors, strengthen the research on relevant characteristics, and further point out the production index of fine chemical industry. In order to ensure the effectiveness of chemical production. In recent years, the rapid development of information technology, including big data, cloud computing, industrial interconnection, mobile interconnection, robot, artificial intelligence and deep learning, has created new opportunities for the upgrading of fine chemical process detection and control technology. Therefore, in-depth analysis and study of the characteristics of fine chemical production process and the current situation and development trend of process parameter detection and control technology, and in-depth study of new fine chemical process measurement and control technology will be necessary to improve the comprehensive automation level of fine chemical process and promote the development of fine chemical production technology which will also produce important economic benefits and technical value.
    Type of Medium: Online Resource
    ISSN: 1742-6588 , 1742-6596
    URL: Article
    DOI: 10.1088/1742-6596/1992/2/022152
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 2021
    detail.hit.zdb_id: 2166409-2
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  • 7
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    A pair of non-Mendelian genes at the Ga2 locus confer unilateral cross-incompatibility in maize
    Springer Science and Business Media LLC ; 2022
    In:  Nature Communications Vol. 13, No. 1 ( 2022-04-14)
    Details
    In: Nature Communications, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2022-04-14)
    Abstract: Maize unilateral cross-incompatibility (UCI) that causes non-Mendelian segregation ratios has been documented for more than a century. Ga1 , Ga2 , and Tcb1 are three major UCI systems, described but not fully understood. Here, we report comprehensive genetic studies on the Ga2 locus and map-based cloning of the tightly linked male determinant ZmGa2P and female determinant ZmGa2F that govern pollen-silk compatibility among different maize genotypes. Both determinants encode putative pectin methylesterases (PME). A significantly higher degree of methyl esterification is detected in the apical region of pollen tubes growing in incompatible silks. No direct interaction between ZmGa2P and ZmGa2F is detected in the yeast two-hybrid system implying a distinct mechanism from that of self-incompatibility (SI). We also demonstrate the feasibility of Ga2 as a reproductive barrier in commercial breeding programs and stacking Ga2 with Ga1 could strengthen the UCI market potentials.
    Type of Medium: Online Resource
    ISSN: 2041-1723
    URL: Article
    DOI: 10.1038/s41467-022-29729-z
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2553671-0
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  • 8
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    Deficiency of monoclonal non‐specific suppressor factor beta (MNSFB) promotes pregnancy loss in mice
    Wiley ; 2015
    In:  Molecular Reproduction and Development Vol. 82, No. 6 ( 2015-06), p. 475-488
    Details
    In: Molecular Reproduction and Development, Wiley, Vol. 82, No. 6 ( 2015-06), p. 475-488
    Abstract: Maternal immune tolerance to the semi‐allogenic fetus is required for successful pregnancy in mammals. Monoclonal nonspecific suppressor factor beta (MNSFB) is an immunosuppressive factor present in uterine epithelial and stromal cells, as well as in macrophages and T cells. Although the functional neutralization of MNSFB using specific antibodies against it lead to failed embryo implantation in mice, the exact role of MNSFB at the fetal–maternal interface remains unclear. The present study generated conditional heterozygous Mnsfb ‐deficient ( Mnsfb +/ − ) mice using the LoxP/Cre system. Western‐blot analyses showed that uterine MNSFB protein in Mnsfb +/− mice was remarkably down‐regulated compared to that in the wild‐type ( Mnsfb +/+ ) mice. The litter size of female Mnsfb +/− mice was significantly reduced, which corresponded to developmental failure of embryos beyond Day 11 of pregnancy. The expression level of MNSFB protein was also lower in the failing compared to the normal embryos. An aberrant interaction between the embryos of Day‐4 pregnant wild‐type mice and endometrial stromal cells of female Mnsfb +/− mice was observed in vitro. The uterine Day‐5 abundance of P53, BAX, and BCL‐G in pregnant Mnsfb +/− mice was significantly decreased compared to that of wild‐type mice, whereas the expression of P27 and tumor necrosis factor alpha (TNFA) was elevated. By comparison, the levels of MNSFB and BAX proteins in human decidual tissues obtained from recurrent spontaneous miscarriage patients were significantly reduced compared to those obtained from legal medial abortion, highlighting the involvement of MNSFB in the pathogenesis of recurrent spontaneous miscarriage. Together, these results demonstrated that a deficiency in MNSFb is associated with pregnancy loss, probably through reduced P53 and/or increased TNFA production at the fetal–maternal interface. Mol. Reprod. Dev. 82: 475–488, 2015. © 2015 Wiley Periodicals, Inc .
    Type of Medium: Online Resource
    ISSN: 1040-452X , 1098-2795
    URL: Issue
    URL: Article
    DOI: 10.1002/mrd.v82.6
    DOI: 10.1002/mrd.22495
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 1493888-1
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  • 9
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    A single silk‐ and multiple pollen‐expressed PMEs at the Ga1 locus modulate maize unilateral cross‐incompatibility
    Wiley ; 2023
    In:  Journal of Integrative Plant Biology Vol. 65, No. 5 ( 2023-05), p. 1344-1355
    Details
    In: Journal of Integrative Plant Biology, Wiley, Vol. 65, No. 5 ( 2023-05), p. 1344-1355
    Abstract: The Gametophyte factor1 ( Ga1 ) locus in maize confers unilateral cross‐incompatibility (UCI), and it is controlled by both pollen and silk‐specific determinants. Although the Ga1 locus has been reported for more than a century and is widely utilized in maize breeding programs, only the pollen‐specific ZmGa1P has been shown to function as a male determinant; thus, the genomic structure of the Ga1 locus and all the determinants that control UCI at this locus have not yet been fully characterized. Here, we used map‐based cloning to confirm the determinants of UCI at the Ga1 locus and maize pan‐genome sequence data to characterize the genomic structure of the Ga1 locus. The Ga1 locus comprises one silk‐expressed pectin methylesterase gene ( PME ) ( ZmGa1F ) and eight pollen‐expressed PME s ( ZmGa1P and ZmGa1PL1 ‐ 7 ). Knockout of ZmGa1F in Ga1 / Ga1 lines leads to the complete loss of the female barrier function. The expression of individual ZmGa1PL genes in a ga1 / ga1 background endows ga1 pollen with the ability to overcome the female barrier of the Ga1 locus. These findings, combined with genomic data and genetic analyses, indicate that the Ga1 locus is modulated by a single female determinant and multiple male determinants, which are tightly linked. The results of this study provide valuable insights into the genomic structure of the Ga2 and Tcb1 loci and will aid applications of these loci in maize breeding programs.
    Type of Medium: Online Resource
    ISSN: 1672-9072 , 1744-7909
    URL: Issue
    URL: Article
    DOI: 10.1111/jipb.v65.5
    DOI: 10.1111/jipb.13445
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2130095-1
    SSG: 12
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  • 10
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    Repellency of forty‐one aromatic plant species to the Asian citrus psyllid, vector of the bacterium associated with huanglongbing
    Wiley ; 2020
    In:  Ecology and Evolution Vol. 10, No. 23 ( 2020-12), p. 12940-12948
    Details
    In: Ecology and Evolution, Wiley, Vol. 10, No. 23 ( 2020-12), p. 12940-12948
    Abstract: Huanglongbing (HLB) is the most devastating citrus disease worldwide. The organism associated with the disease is spread by an insect vector, Diaphorina citri , commonly known as Asian citrus psyllid (ACP). Current management of HLB relies either on physical removal of the infected plants or on chemical control of ACP. Both methods are costly and not overly effective. In addition, public concerns regarding insecticide residues in fruit have greatly increased in recent years. It has been hypothesized that plant volatiles could act as repellents to ACP, thus reduce the incidence of HLB. To test this hypothesis, the repellency of fresh tissues of 41 aromatic plant species to ACP was investigated. The repellency of individual species was determined using a Y‐tube olfactometer. Our results showed that volatiles of five plant species were highly effective in repelling ACP with repellency as much as 76%. Among these, the tree species, Camptotheca acuminate , a nd the two shrubs, Lantana camara and Mimosa bimucronata , could potentially be planted as a landscape barrier. The two herbs, Capsicum annuum and Gynura bicolor , could potentially be used as interplantings in orchards. This is the first time that the repellency of fresh tissues from a diverse range of plant species to ACP has been determined. Although further field evaluation of various interplanting regimes and landscape barriers are needed to assess their effectiveness, our results showed that these aromatic species, being highly repellent to ACP, offer great potential as more cost‐effective and environmentally sustainable alternatives to the current methods of managing HLB.
    Type of Medium: Online Resource
    ISSN: 2045-7758 , 2045-7758
    URL: Issue
    URL: Article
    DOI: 10.1002/ece3.v10.23
    DOI: 10.1002/ece3.6876
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2635675-2
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