In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2022-7-28), p. e0271202-
Abstract:
Cell survival or death is one of the key scientific issues of inflammatory response. To regulate cell death during the occurrence and development of periodontitis, various forms of programmed cell death, such as pyroptosis, ferroptosis, necroptosis, and apoptosis, have been proposed. It has been found that ferroptosis characterized by iron-dependent lipid peroxidation is involved in cancer, degenerative brain diseases and inflammatory diseases. Furthermore, NCOA4 is considered one of ferroptosis-related genes (FRGs) contributing to butyrate-induced cell death in the periodontitis. This research aims to analyze the expression of FRGs in periodontitis tissues and to explore the relationship between ferroptosis and periodontitis. Method Genes associated with periodontitis were retrieved from two Gene Expression Omnibus datasets. Then, we normalized microarray data and removed the batch effect using the R software. We used R to convert the mRNA expression data and collected the expression of FRGs. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), transcription factor (TF) and protein-protein interaction (PPI) network analyses were used. In addition, we constructed a receiver operating characteristic curve and obtained relative mRNA expression verified by quantitative reverse-transcription polymerase chain reaction (PCR). Results Eight and 10 FRGs related to periodontitis were upregulated and downregulated, respectively. GO analysis showed that FRGs were enriched in the regulation of glutathione biosynthetic, glutamate homeostasis, and endoplasmic reticulum-nucleus signaling pathway. The top TFs included CEBPB , JUND , ATF2 . Based on the PPI network analysis, FRGs were mainly linked to the negative regulation of IRE1-mediated unfolded protein response, regulation of type IIa hypersensitivity, and regulation of apoptotic cell clearance. The expression levels of NCOA4 , SLC1A5 and HSPB1 using PCR were significantly different between normal gingival samples and periodontitis samples. Furthermore, the diagnostic value of FRGs for periodontitis were “Good”. Conclusions We found significant associations between FRGs and periodontitis. The present study not only provides a new possible pathomechanism for the occurrence of periodontitis but also offers a new direction for the diagnosis and treatment of periodontitis.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0271202
DOI:
10.1371/journal.pone.0271202.g001
DOI:
10.1371/journal.pone.0271202.g002
DOI:
10.1371/journal.pone.0271202.g003
DOI:
10.1371/journal.pone.0271202.g004
DOI:
10.1371/journal.pone.0271202.g005
DOI:
10.1371/journal.pone.0271202.g006
DOI:
10.1371/journal.pone.0271202.g007
DOI:
10.1371/journal.pone.0271202.g008
DOI:
10.1371/journal.pone.0271202.g009
DOI:
10.1371/journal.pone.0271202.g010
DOI:
10.1371/journal.pone.0271202.t001
DOI:
10.1371/journal.pone.0271202.t002
DOI:
10.1371/journal.pone.0271202.t003
DOI:
10.1371/journal.pone.0271202.s001
DOI:
10.1371/journal.pone.0271202.s002
DOI:
10.1371/journal.pone.0271202.s003
DOI:
10.1371/journal.pone.0271202.s004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
Permalink