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  • 1
    Online Resource
    Online Resource
    m 6 A promotes planarian regeneration
    Wiley ; 2023
    In:  Cell Proliferation Vol. 56, No. 5 ( 2023-05)
    Details
    In: Cell Proliferation, Wiley, Vol. 56, No. 5 ( 2023-05)
    Abstract: Regeneration is the regrowth of damaged tissues or organs, a vital process in response to damages from primitive organisms to higher mammals. Planarian possesses active whole‐body regenerative capability owing to its vast reservoir of adult stem cells, neoblasts, providing an ideal model to delineate the underlying mechanisms for regeneration. RNA N 6 ‐methyladenosine (m 6 A) modification participates in many biological processes, including stem cell self‐renewal and differentiation, in particular the regeneration of haematopoietic stem cells and axons. However, how m 6 A controls regeneration at the whole‐organism level remains largely unknown. Here, we demonstrate that the depletion of m 6 A methyltransferase regulatory subunit wtap abolishes planarian regeneration, potentially through regulating genes related to cell–cell communication and cell cycle. Single‐cell RNA‐seq (scRNA‐seq) analysis unveils that the wtap knockdown induces a unique type of neural progenitor‐like cells (NP‐like cells), characterized by specific expression of the cell–cell communication ligand grn . Intriguingly, the depletion of m 6 A‐modified transcripts grn , cdk9 or cdk7 partially rescues the defective regeneration of planarian caused by wtap knockdown. Overall, our study reveals an indispensable role of m 6 A modification in regulating whole‐organism regeneration.
    Type of Medium: Online Resource
    ISSN: 0960-7722 , 1365-2184
    URL: Issue
    URL: Article
    DOI: 10.1111/cpr.v56.5
    DOI: 10.1111/cpr.13481
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2019986-7
    detail.hit.zdb_id: 1064202-X
    SSG: 12
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  • 2
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    Integrated analysis of 454 and Illumina transcriptomic sequencing characterizes carbon flux and energy source for fatty acid synthesis in developing Lindera glauca fruits for woody biodiesel
    Springer Science and Business Media LLC ; 2017
    In:  Biotechnology for Biofuels Vol. 10, No. 1 ( 2017-12)
    Details
    In: Biotechnology for Biofuels, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2017-12)
    Type of Medium: Online Resource
    ISSN: 1754-6834
    URL: Article
    DOI: 10.1186/s13068-017-0820-2
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2421351-2
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  • 3
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    N6-methyladenosine RNA modification suppresses antiviral innate sensing pathways via reshaping double-stranded RNA
    Springer Science and Business Media LLC ; 2021
    In:  Nature Communications Vol. 12, No. 1 ( 2021-03-11)
    Details
    In: Nature Communications, Springer Science and Business Media LLC, Vol. 12, No. 1 ( 2021-03-11)
    Abstract: Double-stranded RNA (dsRNA) is a virus-encoded signature capable of triggering intracellular Rig-like receptors (RLR) to activate antiviral signaling, but whether intercellular dsRNA structural reshaping mediated by the N 6 -methyladenosine (m 6 A) modification modulates this process remains largely unknown. Here, we show that, in response to infection by the RNA virus Vesicular Stomatitis Virus (VSV), the m 6 A methyltransferase METTL3 translocates into the cytoplasm to increase m 6 A modification on virus-derived transcripts and decrease viral dsRNA formation, thereby reducing virus-sensing efficacy by RLRs such as RIG-I and MDA5 and dampening antiviral immune signaling. Meanwhile, the genetic ablation of METTL3 in monocyte or hepatocyte causes enhanced type I IFN expression and accelerates VSV clearance. Our findings thus implicate METTL3-mediated m 6 A RNA modification on viral RNAs as a negative regulator for innate sensing pathways of dsRNA, and also hint METTL3 as a potential therapeutic target for the modulation of anti-viral immunity.
    Type of Medium: Online Resource
    ISSN: 2041-1723
    URL: Article
    DOI: 10.1038/s41467-021-21904-y
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2553671-0
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  • 4
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    Chemical perturbations reveal that RUVBL2 regulates the circadian phase in mammals
    American Association for the Advancement of Science (AAAS) ; 2020
    In:  Science Translational Medicine Vol. 12, No. 542 ( 2020-05-06)
    Details
    In: Science Translational Medicine, American Association for the Advancement of Science (AAAS), Vol. 12, No. 542 ( 2020-05-06)
    Abstract: Transcriptional regulation lies at the core of the circadian clockwork, but how the clock-related transcription machinery controls the circadian phase is not understood. Here, we show both in human cells and in mice that RuvB-like ATPase 2 (RUVBL2) interacts with other known clock proteins on chromatin to regulate the circadian phase. Pharmacological perturbation of RUVBL2 with the adenosine analog compound cordycepin resulted in a rapid-onset 12-hour clock phase-shift phenotype at human cell, mouse tissue, and whole-animal live imaging levels. Using simple peripheral injection treatment, we found that cordycepin penetrated the blood-brain barrier and caused rapid entrainment of the circadian phase, facilitating reduced duration of recovery in a mouse jet-lag model. We solved a crystal structure for human RUVBL2 in complex with a physiological metabolite of cordycepin, and biochemical assays showed that cordycepin treatment caused disassembly of an interaction between RUVBL2 and the core clock component BMAL1. Moreover, we showed with spike-in ChIP-seq analysis and binding assays that cordycepin treatment caused disassembly of the circadian super-complex, which normally resides at E-box chromatin loci such as PER1 , PER2 , DBP, and NR1D1 . Mathematical modeling supported that the observed type 0 phase shifts resulted from derepression of E-box clock gene transcription.
    Type of Medium: Online Resource
    ISSN: 1946-6234 , 1946-6242
    URL: Article
    DOI: 10.1126/scitranslmed.aba0769
    Language: English
    Publisher: American Association for the Advancement of Science (AAAS)
    Publication Date: 2020
    detail.hit.zdb_id: 2518839-2
    detail.hit.zdb_id: 2518854-9
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  • 5
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    Detailed Imaging of a Seismogenic Fault That Potentially Induced the Two 2019 Weiyuan Moderate Earthquakes in the Sichuan Basin, China
    Seismological Society of America (SSA) ; 2023
    In:  Seismological Research Letters ( 2023-01-25)
    Details
    In: Seismological Research Letters, Seismological Society of America (SSA), ( 2023-01-25)
    Abstract: Induced earthquakes are becoming a significant issue. Several destructive induced earthquakes (Ms ≥5.0) have occurred in shale gas fields located at the southwestern Sichuan basin, China, in the recent years. Specifically, two moderate earthquakes with magnitudes of Ms 5.4 (occurred on 8 September 2019) and 5.2 (occurred on 18 December 2019) exceeded the largest earthquake ever recorded in the Weiyuan. It remains unclear which seismogenic faults were responsible for the two moderate events due to insufficient knowledge of the subsurface structure and contradictory hypocenter depths obtained from different seismological methods. In this study, mainshock locations, focal mechanisms, and precise relocations of microseismicity were used to map the 3D geometry of the seismogenic fault. The result shows that the two moderate earthquakes occurred on the same fault. High-resolution seismic reflection profiles indicate several preexisting faults at various depths and a potential seismogenic fault. No large-scale faults are identified in the sedimentary cover above the Cambrian at the shallow centroid depth of these earthquakes. However, the seismic reflectors show phase discontinuous feature, indicating the existence of preexisting faults in the basement. A preexisting fault in the basement is consistent with a seismogenic fault that may have induced the two moderate earthquakes. Our study suggests that poroelastic stress propagation is likely responsible for the basement fault reactivation. The rupture on a stressed basement fault was triggered and produced two moderate earthquakes in a short period during hydraulic fracturing. The previous strategy of avoiding direct injection of fluids into preexisting faults to reduce seismic risk in operation region is not enough to avoid the possibility of the coupled poroelastic stress triggering deep fault rupture. We need to reevaluate the potential seismic risk of shale gas development in Weiyuan, the southern Sichuan basin shale gas field considering the preexisting faults in sedimentary and in basement and the effect of the fully coupled poroelastic stress field.
    Type of Medium: Online Resource
    ISSN: 0895-0695 , 1938-2057
    URL: Article
    DOI: 10.1785/0220220228
    Language: English
    Publisher: Seismological Society of America (SSA)
    Publication Date: 2023
    detail.hit.zdb_id: 2403376-5
    detail.hit.zdb_id: 1147385-X
    SSG: 16,13
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  • 6
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    Identification of the Hub Genes Involved in Stem Cell Treatment for Intervertebral Disc Degeneration: A Conjoint Analysis of Single-Cell and Machine Learning
    Wiley ; 2023
    In:  Stem Cells International Vol. 2023 ( 2023-1-24), p. 1-11
    Details
    In: Stem Cells International, Wiley, Vol. 2023 ( 2023-1-24), p. 1-11
    Abstract: Intervertebral disc degeneration (IDD), which is distinguished by a variety of pathologic alterations, is the major cause of low back pain (LBP). Nonetheless, preventative measures or therapies that may delay IDD are scarcely available. In this study, we sought to identify new diagnostic biological markers for IDD. In this first-of-a-kind study combining machine learning, stem cell treatment samples and single-cell sequencing data were collected. Differentially expressed genes (DEGs) were detected from the treatment group and clusters. To filter potential markers, support vector machine analysis and LASSO were performed. LAPTM5 was found to be the hub gene for IDD. In addition, the results of single-cell sequencing demonstrated the critical function of stem cells in IDD. Finally, we found that aging is significantly associated with the rate of stem cells. In general, our results may offer fresh insights that may be used in the investigation of innovative markers for diagnosing IDD. The critical genes identified by the machine learning algorithm could provide new perspectives on IDD.
    Type of Medium: Online Resource
    ISSN: 1687-9678 , 1687-966X
    URL: Article
    DOI: 10.1155/2023/7055264
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2573856-2
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  • 7
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    Impact of the Global Budget Payment System on Expenditure of Cardiovascular Diseases: An Interrupted Time Series Analysis in Shanghai, China
    MDPI AG ; 2019
    In:  International Journal of Environmental Research and Public Health Vol. 16, No. 8 ( 2019-04-17), p. 1385-
    Details
    In: International Journal of Environmental Research and Public Health, MDPI AG, Vol. 16, No. 8 ( 2019-04-17), p. 1385-
    Abstract: Since few studies evaluated the impact of the global budget payment system (GBPS) over time, and by expenditure type, this paper aims to evaluate the impact of the GBPS on expenditure of inpatients, and explores how hospitals curb the expenditure in patients with cardiovascular diseases (CVDs) in Shanghai. We built a time series model with the monthly expenditure of CVDs from 2009 to 2012. We evaluated the instant impact and trends impact of the GBPS and analyzed results based on medical expenditure types (e.g., drug, examination, cure, unclassified items), discharge number, and expenditure per capita. We found GBPS instantly dropped the medical expenditure by Chinese Yuan (CNY) 55.71 million (p 〈 0.001), and decreased the monthly increasing trend by CNY 4.23 million (p = 0.011). The discharge number had 10.4% instant reduction and 225.55 monthly decrease (p = 0.021) while the expenditure per capita experienced fewer changes. Moreover, the expenditure of drug and cure had an instant reduction of CNY 28.31 million and 16.28 million (p 〈 0.001). In conclusion, we considered the GBPS is an effective solution to control the expenditure of CVDs by decreasing the discharge number, and a focus on the drug and cure expenditures lead to greater spend reduction than other types of expenditures.
    Type of Medium: Online Resource
    ISSN: 1660-4601
    URL: Article
    DOI: 10.3390/ijerph16081385
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2175195-X
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  • 8
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    Characterization of free and bound volatile compounds in six Ribes nigrum L. blackcurrant cultivars
    Elsevier BV ; 2018
    In:  Food Research International Vol. 103 ( 2018-01), p. 301-315
    Details
    In: Food Research International, Elsevier BV, Vol. 103 ( 2018-01), p. 301-315
    Type of Medium: Online Resource
    ISSN: 0963-9969
    URL: Article
    DOI: 10.1016/j.foodres.2017.10.038
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2018
    detail.hit.zdb_id: 1111695-X
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  • 9
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    Anatomy of provincial level inequality in maternal mortality in China during 2004–2016: a new decomposition analysis
    Springer Science and Business Media LLC ; 2020
    In:  BMC Public Health Vol. 20, No. 1 ( 2020-12)
    Details
    In: BMC Public Health, Springer Science and Business Media LLC, Vol. 20, No. 1 ( 2020-12)
    Abstract: The maternal mortality ratio (MMR) is an important indicator of maternal health and socioeconomic development. Although China has experienced a large decline in MMR, substantial disparities across regions are still apparent. This study aims to explore causes of socioeconomic related inequality in MMR at the province-level in China from 2004 to 2016. Methods We collected data from various issues of the China Health Statistics Yearbook, China Statistics Yearbook, and China Population and Employment Statistics Yearbook to construct a longitudinal sample of all provinces in China. We first examined determinants of the MMR using province fixed-effect models, accounted for socioeconomic condition, health resource allocation, and access to health care. We then used the concentration index (CI) to measure MMR inequality and employed the direct decomposition method to estimate the marginal impact of the determinants on the inequality index. Importance of the determinants were compared based on logworth values. Results During our study period, economically more deprived provinces experienced higher MMR than better-off ones. There was no evidence of improved socioeconomic related inequality in MMR. Illiteracy proportion was positively associated with the MMR ( p   〈  0.01). In contrast, prenatal check-up rate ( p  = 0.05), hospital delivery rate ( p   〈  0.01) and rate of delivery attended by professionals ( p  = 0.02) were negatively associated with the MMR. We also find that higher maternal health profile creation rate ( p   〈  0.01) was associated with a pro-poor change of MMR inequality. Conclusion Access to healthcare was the most important factor in explaining the persistent MMR inequality in China, followed by socioeconomic condition. We do not find evidence that health resource allocation was a contributing factor.
    Type of Medium: Online Resource
    ISSN: 1471-2458
    URL: Article
    DOI: 10.1186/s12889-020-08830-2
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2041338-5
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  • 10
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    m 6 A Regulates Liver Metabolic Disorders and Hepatogenous Diabetes
    Oxford University Press (OUP) ; 2020
    In:  Genomics, Proteomics & Bioinformatics Vol. 18, No. 4 ( 2020-08-01), p. 371-383
    Details
    In: Genomics, Proteomics & Bioinformatics, Oxford University Press (OUP), Vol. 18, No. 4 ( 2020-08-01), p. 371-383
    Abstract: N 6-methyladenosine (m6A) is one of the most abundant modifications on mRNAs and plays important roles in various biological processes. The formation of m6A is catalyzed by a methyltransferase complex (MTC) containing a key factor methyltransferase-like 3 (Mettl3). However, the functions of Mettl3 and m6A modification in hepatic lipid and glucose metabolism remain unclear. Here, we showed that both Mettl3 expression and m6A level increased in the livers of mice with high fat diet (HFD)-induced metabolic disorders. Overexpression of Mettl3 aggravated HFD-induced liver metabolic disorders and insulin resistance. In contrast, hepatocyte-specific knockout of Mettl3 significantly alleviated HFD-induced metabolic disorders by slowing weight gain, reducing lipid accumulation, and improving insulin sensitivity. Mechanistically, Mettl3 depletion-mediated m6A loss caused extended RNA half-lives of metabolism-related genes, which consequently protected mice against HFD-induced metabolic syndrome. Our findings reveal a critical role of Mettl3-mediated m6A in HFD-induced metabolic disorders and hepatogenous diabetes.
    Type of Medium: Online Resource
    ISSN: 1672-0229 , 2210-3244
    URL: Article
    DOI: 10.1016/j.gpb.2020.06.003
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2020
    detail.hit.zdb_id: 2233708-8
    detail.hit.zdb_id: 2240213-5
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