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  • 1
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    Distribution characteristics of the sabA, hofC, homA, homB and frpB-4 genes of Helicobacter pylori in different regions of China
    Public Library of Science (PLoS) ; 2022
    In:  PLOS ONE Vol. 17, No. 5 ( 2022-5-19), p. e0268373-
    Details
    In: PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 5 ( 2022-5-19), p. e0268373-
    Abstract: Helicobacter pylori ( H . pylori ) encodes numerous outer membrane proteins (OMPs), with considerable geographic heterogeneity and related to different clinical outcomes. This study aimed to investigate the distribution characteristics of five important OMP genes ( sabA , hofC , homA , homB and frpB-4 ) in different regions of China. Materials and method A total of 266 strains were isolated from 348 stomach biopsy specimens in Shandong, Guangxi, Heilongjiang, Hunan, and Qinghai provinces. The presence of sabA , hofC , homA , homB and frpB-4 gene was detected by polymerase chain reaction (PCR) from H . pylori genomic DNA. Results Among the strains in five regions, the prevalence of frpB-4 was 100% and that of hofC was 97.7%. The prevalence of homB in the isolates from Qinghai (45.5%) was significantly lower than that in Shandong (75.3%), Guangxi (76.9%) and Hunan (69.6%) ( P 〈 0.05). The frequency of homA in Shandong (30.1%) was significantly lower than in Guangxi (57.7%) and Qinghai (63.6%) ( P 〈 0.05). The prevalence of the sabA gene in Shandong, Guangxi, Heilongjiang, Hunan and Qinghai provinces was 21.9%, 59.7%, 45.9%, 52.2%, and 18.2%, respectively ( P 〈 0.05). The sabA “on” status was significantly more frequent in isolates from Guangxi (46.8%), Heilongjiang (37.8%), and Hunan (47.8%) than Qinghai (3.0%) ( P 〈 0.05). The presence of homA and sabA genes may be negatively correlated with the development of gastritis. There was no significant association between the frpB-4 , hofC , homB gene and clinical outcomes. Conclusion The prevalence of homA , homB , and sabA genes and the sabA “on” or “off” status have significant geographical differences among five provinces in China. The presence of homA and sabA genes may be protective factors of gastritis.
    Type of Medium: Online Resource
    ISSN: 1932-6203
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.1371/journal.pone.0268373
    DOI: 10.1371/journal.pone.0268373.t001
    DOI: 10.1371/journal.pone.0268373.t002
    DOI: 10.1371/journal.pone.0268373.t003
    DOI: 10.1371/journal.pone.0268373.t004
    DOI: 10.1371/journal.pone.0268373.t005
    DOI: 10.1371/journal.pone.0268373.s001
    Language: English
    Publisher: Public Library of Science (PLoS)
    Publication Date: 2022
    detail.hit.zdb_id: 2267670-3
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  • 2
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    Diversity of 3′ variable region of cagA gene in Helicobacter pylori strains isolated from Chinese population
    Springer Science and Business Media LLC ; 2021
    In:  Gut Pathogens Vol. 13, No. 1 ( 2021-12)
    Details
    In: Gut Pathogens, Springer Science and Business Media LLC, Vol. 13, No. 1 ( 2021-12)
    Abstract: The cytotoxin-associated gene A ( cagA ) is one of the most important virulence factors of Helicobacter pylori ( H. pylori ). There is a highly polymorphic Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region in the C-terminal of CagA protein. This repeat region is thought to play an important role in the pathogenesis of gastrointestinal diseases. The aim of this study was to investigate the diversity of cagA 3′ variable region and the amino acid polymorphisms in the EPIYA segments of the CagA C-terminal region of H. pylori , and their association with gastroduodenal diseases. Methods A total of 515 H. pylori strains from patients in 14 different geographical regions of China were collected. The genomic DNA from each strain was extracted and the cagA 3′ variable region was amplified by polymerase chain reaction (PCR). The PCR products were sequenced and analyzed using MEGA 7.0 software. Results A total of 503 (97.7%) H. pylori strains were cagA -positive and 1,587 EPIYA motifs were identified, including 12 types of EPIYA or EPIYA-like sequences. In addition to the four reported major segments, several rare segments (e.g., B′, B″ and D′) were defined and 20 different sequence types (e.g., ABD, ABC) were found in our study. A total of 481 (95.6%) strains carried the East Asian type CagA, and the ABD subtypes were most prevalent (82.1%). Only 22 strains carried the Western type CagA, which included AC, ABC, ABCC and ABCCCC subtypes. The CagA-ABD subtype had statistical difference in different geographical regions (P = 0.006). There were seven amino acid polymorphisms in the sequences surrounding the EPIYA motifs, among which amino acids 893 and 894 had a statistical difference with gastric cancer (P = 0.004). Conclusions In this study, 503 CagA sequences were studied and analyzed in depth. In Chinese population, most H. pylori strains were of the CagA-ABD subtype and its presence was associated with gastroduodenal diseases. Amino acid polymorphisms at residues 893 and 894 flanking the EPIYA motifs had a statistically significant association with gastric cancer.
    Type of Medium: Online Resource
    ISSN: 1757-4749
    URL: Article
    DOI: 10.1186/s13099-021-00419-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2478277-4
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  • 3
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    Long-term investigation of environmental radioactivity levels and public health around the Qinshan Nuclear Power Plant, China
    Springer Science and Business Media LLC ; 2022
    In:  Scientific Reports Vol. 12, No. 1 ( 2022-03-23)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 12, No. 1 ( 2022-03-23)
    Abstract: To evaluate the impact of the Qinshan Nuclear Power Plant (Qinshan NPP) in normal operation on the surrounding environment and population, the radioactivity levels of drinking water and the ambient environment, as well as the residents’ cancer incidence, were continuously monitored for a period of 9 years (2012–2020). All of the gross α and β radioactivity concentrations in drinking water were less than the WHO recommended values (0.5 Bq/L for gross α and 1 Bq/L for gross β ). The results of ambient environment accumulated dose monitored by thermoluminescent dosimeters (TLDs) indicated that the ambient environment radioactive level around the Qinshan NPP is consistently at natural background radiation levels. The age-dependent annual effective doses due to the ingestion of tap water or exposure to the outdoor ambient environment are lower than the reference dose of 0.1 mSv/year. The corresponding excess risks are at relatively low levels. Thus, the consumption of drinking water and outdoor activities are not expected to give rise to any detectable adverse effects on the health of the public around the Qinshan NPP. For all cancers combined, the age-standardized incidence rate by the Chinese 2000 standard population of the inhabitants living around Qinshan NPP is consistent with that of Zhejiang Province as a whole. Based on current radiation risk estimates, radiation exposure is not a plausible explanation for any excess cancers observed in the vicinity of the Qinshan NPP.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-022-09091-2
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2615211-3
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  • 4
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    Global Deletion of TSPO Does Not Affect the Viability and Gene Expression Profile
    Public Library of Science (PLoS) ; 2016
    In:  PLOS ONE Vol. 11, No. 12 ( 2016-12-1), p. e0167307-
    Details
    In: PLOS ONE, Public Library of Science (PLoS), Vol. 11, No. 12 ( 2016-12-1), p. e0167307-
    Type of Medium: Online Resource
    ISSN: 1932-6203
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.1371/journal.pone.0167307
    DOI: 10.1371/journal.pone.0167307.g001
    DOI: 10.1371/journal.pone.0167307.g002
    DOI: 10.1371/journal.pone.0167307.g003
    DOI: 10.1371/journal.pone.0167307.g004
    DOI: 10.1371/journal.pone.0167307.g005
    Language: English
    Publisher: Public Library of Science (PLoS)
    Publication Date: 2016
    detail.hit.zdb_id: 2267670-3
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  • 5
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    RdxA Diversity and Mutations Associated with Metronidazole Resistance of Helicobacter pylori
    American Society for Microbiology ; 2023
    In:  Microbiology Spectrum Vol. 11, No. 2 ( 2023-04-13)
    Details
    In: Microbiology Spectrum, American Society for Microbiology, Vol. 11, No. 2 ( 2023-04-13)
    Abstract: Metronidazole (MNZ) is administered as first-line antibiotic for Helicobacter pylori eradication therapy; however, increasing resistance to MNZ impaired the efficacy. Increasing the dose of MNZ was recommended to overcome low-level resistance, but it was difficult to determine MNZ resistance level simply based on the rdxA gene mutation. In this study, the rdxA sequences of 511 clinical H. pylori strains were analyzed to assess the genotypes associated with MNZ resistance. We observed that the prevalences of rdxA sequences with missense, nonsense, and frameshift mutations were 70.25, 11.35, and 17.03%, respectively. Regarding the amino acid substitutions, T31E, H53R, D59N, L62V, S88P, G98S/N, R131K, and V172I were present in most strains regardless of the resistance phenotype. The correlation analysis showed R16H/C, Y47C, A67V/T, and V204I substitutions were associated with MNZ resistance. The mutation resulting in RdxA truncation was observed in 36.29% of the resistant strains, and 83.45% of these strains displayed high-level MNZ resistance (MIC 〉 256 μg/mL). Moreover, all strains with truncated mutation positions before amino acid 70 expressed high-level MNZ resistance. Our results indicated that most amino acid mutations probably contributed to the sequence diversity of RdxA, while R16H/C, Y47C, A67V/T, and V204I were potentially helpful to identify resistant strains. Although it was difficult to determine the mutations associated with MNZ resistance, the prediction of high-level resistance based on truncated characteristics of RdxA might be an important approach, which can effectively avoid H. pylori eradication therapy with unreasonable of MNZ dose increases for patients with high-level drug resistance. IMPORTANCE The increasing resistance to metronidazole impaired the efficacy of Helicobacter pylori eradication, and increasing the dose of metronidazole was recommended to overcome low-level resistance. For patients infected with highly resistant strains, the current empirical treatments, which generally used metronidazole in double doses or more, appeared impossibly to overcome the resistance and would only increase the incidence of adverse effects. Our results indicated that high-level metronidazole resistance was predominant, and almost half of the patients with high-level drug resistance could avoid usage of metronidazole based on the truncated mutations of RdxA sequences, which can effectively avoid H. pylori eradication therapy with unreasonable increases in the metronidazole dose.
    Type of Medium: Online Resource
    ISSN: 2165-0497
    URL: Article
    DOI: 10.1128/spectrum.03903-22
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2023
    detail.hit.zdb_id: 2807133-5
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  • 6
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    DNA starvation/stationary phase protection protein of Helicobacter pylori as a potential immunodominant antigen for infection detection
    Wiley ; 2023
    In:  Helicobacter Vol. 28, No. 2 ( 2023-04)
    Details
    In: Helicobacter, Wiley, Vol. 28, No. 2 ( 2023-04)
    Abstract: Application of chicken egg yolk immunoglobulin Y (IgY) for Helicobacter pylori ( H. pylori , HP) has gained much interest in recent years. Comparing with for treatment, IgY may be more advantageous when used for H. pylori detection. Methods Nine strains of H. pylori with different genetic backgrounds were inactivated and used to immunize hens, respectively, for the preparation of polyclonal anti‐ H. pylori immunoglobulin Y (anti‐HP IgY). The proteins of H. pylori with reactivity to anti‐HP IgY were detected by Western Blot. The five protein bands that can be well recognized by anti‐HP IgY of each group, and were prevalent in all nine strains were excised from SDS‐PAGE gel, digested and identified by Nano‐HPLC‐MS/MS analysis. The potential of these identified proteins as antigen detection targets was then assessed by sequence analysis. Results Anti‐HP IgY derived from each group of specific strain immunized hens can recognize self‐strain and non‐self‐strain antigens well. Five immunodominant antigens were identified as chaperonin GroEL, flagellin A, urease subunit alpha, peroxiredoxin and DNA starvation/stationary phase protection protein. Sequences analysis showed that both peroxiredoxin and DNA starvation/stationary phase protection protein were present in all 1000 strains of H. pylori queried, and the amino acid sequences were highly conserved. The highest sequence consistency between the DNA starvation/stationary phase protection protein of H. pylori and non‐ Helicobacter organisms was 52.59%, and the consistent sites were scattered and there was no continuous long fragment consensus sequence. Conclusion DNA starvation/stationary phase protection protein was identified as an immunodominant antigen of H. pylori and sequence analysis indicated that it could serve as a potential antigen target for the diagnosis of H. pylori infection.
    Type of Medium: Online Resource
    ISSN: 1083-4389 , 1523-5378
    URL: Issue
    URL: Article
    DOI: 10.1111/hel.v28.2
    DOI: 10.1111/hel.12955
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2020336-6
    SSG: 12
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  • 7
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    Application of 3D modeling and fusion technology of medical image data in image teaching
    Springer Science and Business Media LLC ; 2021
    In:  BMC Medical Education Vol. 21, No. 1 ( 2021-12)
    Details
    In: BMC Medical Education, Springer Science and Business Media LLC, Vol. 21, No. 1 ( 2021-12)
    Abstract: We combined anatomy with imaging, transformed the 2D information of various imaging techniques into 3D information, and form the assessment system of real medical imaging cases in order to make up for the deficiencies in the current teaching of the medical imaging technology students. Methods A total of 460 medical imaging students were selected and randomly divided into two groups. The research group received the teaching of the fusion of the original CT and MR data 3D model and the original image combined with 3D anatomical image. CT and MRI data are imported through load DICOM of 3D slicer. Different tissues and organs are segmented by threshold and watershed algorithm of segment editor module. Models are exported through export / import models and label maps in segmentation. Save the NHDR file of the original data and Obj file of the corresponding model through save the NHDR and corresponding Obj files are loaded into probe 1.0 software. The software can give different colors to the three-dimensional models of different organs or tissues to display the stereo models and related data, and display the hook edges of organ models on coronal, sagittal and axial images. At the same time, annotation can be established in the corresponding anatomical position. Finally, it can be saved as a single file of Hwl, and the teaching can be opened at any time through the program of probe 1.0. Statistical analysis Academic self-efficacy scale and Self-directed learning ability scale was adopted by self-directed learning evaluation scale between two groups. Results Compare the theoretical scores and case analysis scores of the two groups. The scores of the study and control groups were significantly higher than those of the control group. Before the experiment, no significant difference was detected in the self-efficacy of learning ability and learning behavior between the two groups, while after the experiment, these differences between the two groups were statistically significan. Moreover, the learning ability self-efficacy and learning behavior of the two groups of students after the experiment was significantly higher than that before the experiment. The self-efficacy of the learning behavior of the control group was higher after the experiment than that before the experiment, albeit the difference was not statistically significant. Conclusions The modern, information-based and humanized experimental teaching mode will be constantly improved under the support of PACS system in order to optimize the medical imaging teaching activities for the development of modern medical education.
    Type of Medium: Online Resource
    ISSN: 1472-6920
    URL: Article
    DOI: 10.1186/s12909-021-02620-z
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2044473-4
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  • 8
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    Hybrid OFDMA Resource Allocation Scheme for Ensuring Required Level of Proportional Fairness
    Hindawi Limited ; 2020
    In:  Mathematical Problems in Engineering Vol. 2020 ( 2020-10-22), p. 1-19
    Details
    In: Mathematical Problems in Engineering, Hindawi Limited, Vol. 2020 ( 2020-10-22), p. 1-19
    Abstract: The new-generation wireless communication networks are envisioned to offer higher sum data rates along with the required level of fairness. Previous works tend to suffer from a decayed performance as subcarriers become relatively insufficient in allocation to users. To maximize the sum data rates and ensure the required level of proportional fairness, this paper presents a hybrid OFDMA resource allocation scheme which uses Hungarian algorithm combined with a greedy method for subcarrier allocation and uses bee colony optimization for power allocation. The proposed subcarrier allocation scheme can make full use of advantages of both globally optimal Hungarian algorithm in enhancing sum data rates and locally optimal greedy method in maintaining a reasonable fairness level and can make Hungarian algorithm work in a searching mode for further improvement of sum data rates and fairness. The proposed power allocation scheme can converge to the required level of proportional fairness but with higher sum data rates if the subcarrier allocation does not achieve the required fairness. Simulation results show that the proposed scheme can obtain the required level of proportional fairness but with higher sum data rates even if subcarriers are relatively insufficient in allocation to users. Complexity analysis shows the proposed method has moderate complexity.
    Type of Medium: Online Resource
    ISSN: 1563-5147 , 1024-123X
    URL: Article
    DOI: 10.1155/2020/5201545
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2014442-8
    SSG: 11
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