In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 12 ( 2021-12-7), p. e0259588-
Abstract:
Candida albicans is the leading cause of life-threatening bloodstream candidiasis, especially among immunocompromised patients. The reversible morphological transition from yeast to hyphal filaments in response to host environmental cues facilitates C . albicans tissue invasion, immune evasion, and dissemination. Hence, it is widely considered that filamentation represents one of the major virulence properties in C . albicans . We have previously characterized Ppg1, a PP2A-type protein phosphatase that controls filament extension and virulence in C . albicans . This study conducted RNA sequencing analysis of samples obtained from C . albicans wild type and ppg1 Δ / Δ strains grown under filament-inducing conditions. Overall, ppg1 Δ / Δ strain showed 1448 upregulated and 710 downregulated genes, representing approximately one-third of the entire annotated C . albicans genome. Transcriptomic analysis identified significant downregulation of well-characterized genes linked to filamentation and virulence, such as ALS3 , HWP1 , ECE1 , and RBT1 . Expression analysis showed that essential genes involved in C . albicans central carbon metabolisms, including GDH3 , GPD1 , GPD2 , RHR2 , INO1 , AAH1 , and MET14 were among the top upregulated genes. Subsequent metabolomics analysis of C . albicans ppg1 Δ / Δ strain revealed a negative enrichment of metabolites with carboxylic acid substituents and a positive enrichment of metabolites with pyranose substituents. Altogether, Ppg1 in vitro analysis revealed a link between metabolites substituents and filament formation controlled by a phosphatase to regulate morphogenesis and virulence.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0259588
DOI:
10.1371/journal.pone.0259588.g001
DOI:
10.1371/journal.pone.0259588.g002
DOI:
10.1371/journal.pone.0259588.g003
DOI:
10.1371/journal.pone.0259588.t001
DOI:
10.1371/journal.pone.0259588.t002
DOI:
10.1371/journal.pone.0259588.t003
DOI:
10.1371/journal.pone.0259588.s001
DOI:
10.1371/journal.pone.0259588.s002
DOI:
10.1371/journal.pone.0259588.r001
DOI:
10.1371/journal.pone.0259588.r002
DOI:
10.1371/journal.pone.0259588.r003
DOI:
10.1371/journal.pone.0259588.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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