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  • 1
    Online Resource
    Online Resource
    Vimentin Is an Attachment Receptor for Mycoplasma pneumoniae P1 Protein
    American Society for Microbiology ; 2023
    In:  Microbiology Spectrum Vol. 11, No. 2 ( 2023-04-13)
    Details
    In: Microbiology Spectrum, American Society for Microbiology, Vol. 11, No. 2 ( 2023-04-13)
    Abstract: Mycoplasma pneumoniae is the most common pathogen causing respiratory tract infection, and the P1 protein on its adhesion organelle plays a crucial role during the pathogenic process. Currently, there are many studies on P1 and receptors on host cells, but the adhesion mechanism of P1 protein is still unclear. In this study, a modified virus overlay protein binding assay (VOPBA) and liquid chromatography-mass spectrometry (LC-MS) were performed to screen for proteins that specifically bind to the region near the carboxyl terminus of the recombinant P1 protein (rP1-C). The interaction between rP1-C and vimentin or β-4-tubulin were confirmed by far-Western blotting and coimmunoprecipitation. Results verified that vimentin and β-4-tubulin were mainly distributed on the cell membrane and cytoplasm of human bronchial epithelial (BEAS-2B) cells, but only vimentin could interact with rP1-C. The results of the adhesion and adhesion inhibition assays indicated that the adhesion of M. pneumoniae and rP1-C to cells could be partly inhibited by vimentin and its antibody. When vimentin was downregulated with the corresponding small interfering RNA (siRNA) or overexpressed in BEAS-2B cells, the adhesion of M. pneumoniae and rP1-C to cells was decreased or increased, respectively, which indicated that vimentin was closely associated with the adhesion of M. pneumoniae and rP1-C to BEAS-2B cells. Our results demonstrate that vimentin could be a receptor on human bronchial epithelial cells for the P1 protein and plays an essential role in the adhesion of M. pneumoniae to cells, which may clarify the pathogenesis of M. pneumoniae . IMPORTANCE Mycoplasma pneumoniae is the most common pathogen causing respiratory tract infection, and the P1 protein on its adhesion organelle plays a crucial role during the pathogenic process. A variety of experiments, including enzyme-linked immunosorbent assay (ELISA), coimmunoprecipitation, adhesion, and adhesion inhibition assay, have demonstrated that the M. pneumoniae P1 protein can interact with vimentin, that the adhesion of M. pneumoniae and recombinant P1 protein to BEAS-2B cells was affected by the expression level of vimentin. This provides a new idea for the prevention and treatment of Mycoplasma pneumoniae infection.
    Type of Medium: Online Resource
    ISSN: 2165-0497
    URL: Article
    DOI: 10.1128/spectrum.04489-22
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2023
    detail.hit.zdb_id: 2807133-5
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  • 2
    Online Resource
    Online Resource
    Identifying agronomic practices with higher yield and lower global warming potential in rice paddies: a global meta-analysis
    Elsevier BV ; 2021
    In:  Agriculture, Ecosystems & Environment Vol. 322 ( 2021-12), p. 107663-
    Details
    In: Agriculture, Ecosystems & Environment, Elsevier BV, Vol. 322 ( 2021-12), p. 107663-
    Type of Medium: Online Resource
    ISSN: 0167-8809
    URL: Article
    DOI: 10.1016/j.agee.2021.107663
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2013743-6
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Novel acsF Gene Primers Revealed a Diverse Phototrophic Bacterial Population, Including Gemmatimonadetes, in Lake Taihu (China)
    American Society for Microbiology ; 2016
    In:  Applied and Environmental Microbiology Vol. 82, No. 18 ( 2016-09-15), p. 5587-5594
    Details
    In: Applied and Environmental Microbiology, American Society for Microbiology, Vol. 82, No. 18 ( 2016-09-15), p. 5587-5594
    Abstract: Anoxygenic phototrophs represent an environmentally important and phylogenetically diverse group of organisms. They harvest light using bacteriochlorophyll-containing reaction centers. Recently, a novel phototrophic bacterium, Gemmatimonas phototrophica , belonging to a rarely studied phylum, Gemmatimonadetes , was isolated from a freshwater lake in the Gobi Desert. To obtain more information about the environmental distribution of phototrophic Gemmatimonadetes , we collected microbial samples from the water column, upper sediment, and deeper anoxic sediment of Lake Taihu, China. MiSeq sequencing of the 16S rRNA, pufM , and bchY genes was carried out to assess the diversity of local phototrophic communities. In addition, we designed new degenerate primers of aerobic cyclase gene acsF , which serves as a convenient marker for both phototrophic Gemmatimonadetes and phototrophic Proteobacteria . Our results showed that most of the phototrophic species in Lake Taihu belong to Alpha - and Betaproteobacteria . Sequences of green sulfur and green nonsulfur bacteria (phototrophic Chlorobi and Chloroflexi , respectively) were found in the sediment. Using the newly designed primers, we identified a diverse community of phototrophic Gemmatimonadetes forming 30 operational taxonomic units. These species represented 10.5 and 17.3% of the acsF reads in the upper semiaerobic sediment and anoxic sediment, whereas their abundance in the water column was 〈 1%. IMPORTANCE Photosynthesis is one of the most fundamental biological processes on Earth. Recently, the presence of photosynthetic reaction centers has been reported from a rarely studied bacterial phylum, Gemmatimonadetes , but almost nothing is known about the diversity and environmental distribution of these organisms. The newly designed acsF primers were used to identify phototrophic Gemmatimonadetes from planktonic and sediment samples collected in Lake Taihu, China. The Gemmatimonadetes sequences were found mostly in the upper sediments, documenting the preference of Gemmatimonadetes for semiaerobic conditions. Our results also show that the phototrophic Gemmatimonadetes present in Lake Taihu were relatively diverse, encompassing 30 operational taxonomic units.
    Type of Medium: Online Resource
    ISSN: 0099-2240 , 1098-5336
    URL: Article
    DOI: 10.1128/AEM.01063-16
    RVK:
    WA 15000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2016
    detail.hit.zdb_id: 223011-2
    detail.hit.zdb_id: 1478346-0
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Transcriptomic, proteomic, and physiological comparative analyses of flooding mitigation of the damage induced by low-temperature stress in direct seeded early indica rice at the seedling stage
    Springer Science and Business Media LLC ; 2021
    In:  BMC Genomics Vol. 22, No. 1 ( 2021-03-12)
    Details
    In: BMC Genomics, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2021-03-12)
    Abstract: Low temperature (LT) often occurs at the seedling stage in the early rice-growing season, especially for direct seeded early-season indica rice, and using flooding irrigation can mitigate LT damage in rice seedlings. The molecular mechanism by which flooding mitigates the damage induced by LT stress has not been fully elucidated. Thus, LT stress at 8 °C, LT accompanied by flooding (LTF) and CK (control) treatments were established for 3 days to determine the transcriptomic, proteomic and physiological response in direct seeded rice seedlings at the seedling stage. Results LT damaged chloroplasts, and thylakoid lamellae, and increased osmiophilic bodies and starch grains compared to CK, but LTF alleviated the damage to chloroplast structure caused by LT. The physiological characteristics of treated plants showed that compared with LT, LTF significantly increased the contents of rubisco, chlorophyll, PEPCK, ATP and GA 3 but significantly decreased soluble protein, MDA and ABA contents. 4D-label-free quantitative proteomic profiling showed that photosynthesis-responsive proteins, such as phytochrome, as well as chlorophyll and the tricarboxylic acid cycle were significantly downregulated in LT/CK and LTF/CK comparison groups. However, compared with LT, phytochrome, chlorophyllide oxygenase activity and the glucan branching enzyme in LTF were significantly upregulated in rice leaves. Transcriptomic and proteomic studies identified 72,818 transcripts and 5639 proteins, and 4983 genes that were identified at both the transcriptome and proteome levels. Differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were significantly enriched in glycine, serine and threonine metabolism, biosynthesis of secondary metabolites, glycolysis/gluconeogenesis and metabolic pathways. Conclusion Through transcriptomic, proteomic and physiological analyses, we determined that a variety of metabolic pathway changes were induced by LT and LTF. GO and KEGG enrichment analyses demonstrated that DEGs and DEPs were associated with photosynthesis pathways, antioxidant enzymes and energy metabolism pathway-related proteins. Our study provided new insights for efforts to reduce the damage to direct seeded rice caused by low-temperature stress and provided a breeding target for low temperature flooding-resistant cultivars. Further analysis of translational regulation and metabolites may help to elucidate the molecular mechanisms by which flooding mitigates low-temperature stress in direct seeded early indica rice at the seedling stage.
    Type of Medium: Online Resource
    ISSN: 1471-2164
    URL: Article
    DOI: 10.1186/s12864-021-07458-9
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2041499-7
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Mycoplasma genitalium Protein of Adhesion Suppresses T Cell Activation via CypA-CaN-NFAT Pathway
    American Society for Microbiology ; 2023
    In:  Microbiology Spectrum Vol. 11, No. 3 ( 2023-06-15)
    Details
    In: Microbiology Spectrum, American Society for Microbiology, Vol. 11, No. 3 ( 2023-06-15)
    Abstract: Mycoplasma genitalium is a prokaryotic microorganism that causes urogenital tract infections. M. genitalium protein of adhesion (MgPa) was essential for M. genitalium attachment and subsequent invasion into host cells. Our prior research confirmed that Cyclophilin A (CypA) was the binding receptor for MgPa and MgPa-CypA interaction can lead to the production of inflammatory cytokines. In this study, we revealed that the recombinant MgPa (rMgPa) could inhibit the CaN-NFAT signaling pathway to reduce the level of IFN-γ, IL-2, CD25, and CD69 in Jurkat cells by binding to the CypA receptor. Moreover, rMgPa inhibited the expressions of IFN-γ, IL-2, CD25, and CD69 in primary mouse T cells. Likewise, the expressions of these T cells activation-related molecules in CypA-siRNA-transfected cells and CypA −/− mouse primary T cell was strengthened by rMgPa. These findings showed that rMgPa suppressed T cell activation by downregulating the CypA-CaN-NFAT pathway, and as a result, acted as an immunosuppressive agent. IMPORTANCE Mycoplasma genitalium is a sexually transmitted bacterium that can co-infect with other infections and causes nongonococcal urethritis in males, cervicitis, pelvic inflammatory disease, premature birth, and ectopic pregnancy in women. The adhesion protein of M. genitalium (MgPa) is the primary virulence factor in the complicated pathogenicity of M. genitalium . This research proved that MgPa could interact with host cell Cyclophilin A (CypA) and prevent T cell activation by inhibiting Calcineurin (CaN) phosphorylation and NFAT nuclear translocation, which clarified the immunosuppression mechanism of M. genitalium to host T cells. Therefore, this study can provide a new idea that CypA can be used for a therapeutic or prophylactic target for M. genitalium infection.
    Type of Medium: Online Resource
    ISSN: 2165-0497
    URL: Article
    DOI: 10.1128/spectrum.04503-22
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2023
    detail.hit.zdb_id: 2807133-5
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  • 6
    Online Resource
    Online Resource
    Foliar application uniconazole enhanced lodging resistance of high-quality indica rice (Oryza sativa L. ) by altering anatomical traits, cell structure and endogenous hormones
    Elsevier BV ; 2022
    In:  Field Crops Research Vol. 277 ( 2022-03), p. 108425-
    Details
    In: Field Crops Research, Elsevier BV, Vol. 277 ( 2022-03), p. 108425-
    Type of Medium: Online Resource
    ISSN: 0378-4290
    URL: Article
    DOI: 10.1016/j.fcr.2021.108425
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 2012484-3
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  • 7
    Online Resource
    Online Resource
    Effects of free-air temperature increase on grain yield and greenhouse gas emissions in a double rice cropping system
    Elsevier BV ; 2022
    In:  Field Crops Research Vol. 281 ( 2022-05), p. 108489-
    Details
    In: Field Crops Research, Elsevier BV, Vol. 281 ( 2022-05), p. 108489-
    Type of Medium: Online Resource
    ISSN: 0378-4290
    URL: Article
    DOI: 10.1016/j.fcr.2022.108489
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 2012484-3
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  • 8
    Online Resource
    Online Resource
    The Influence of Post-Traumatic Growth on College Students’ Creativity During the COVID-19 Pandemic: The Mediating Role of General Self-Efficacy and the Moderating Role of Deliberate Rumination
    Frontiers Media SA ; 2021
    In:  Frontiers in Psychology Vol. 12 ( 2021-4-14)
    Details
    In: Frontiers in Psychology, Frontiers Media SA, Vol. 12 ( 2021-4-14)
    Abstract: Purpose: This study used a moderated mediation model to test the mediating effect of general self-efficacy on the relationship between post-traumatic growth (PTG) and creativity and the moderating effect of deliberate rumination in the second path of the indirect mediation path during the COVID-19 pandemic. Methods: A sample of 881 university students from Guangdong Province, China, was surveyed with the Posttraumatic Growth Inventory, the Runco Ideational Behavior Scale, the General Self-Efficacy Scale, and the Deliberate Rumination Inventory. SPSS (23 version) and PROCESS (3.3 version) were used for correlation analyses, mediation analysis, and moderated mediation analysis. Results: (1) PTG was positively correlated with creativity, self-efficacy, and deliberate rumination. Creativity was positively correlated with self-efficacy and deliberate rumination. Deliberate rumination was positively correlated with self-efficacy. (2) Self-efficacy mediated the relationship between PTG and creativity. (3) Deliberate rumination moderated the second half of the path of “PTG → self-efficacy → creativity.” Conclusions: PTG affected creativity directly and also indirectly through self-efficacy. In particular, deliberate rumination moderated the relationship between self-efficacy and creativity, such that the association was stronger when the incidence of deliberate rumination was low. These results provide a more comprehensive understanding of the positive link between PTG and creativity.
    Type of Medium: Online Resource
    ISSN: 1664-1078
    URL: Article
    DOI: 10.3389/fpsyg.2021.665973
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2563826-9
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  • 9
    Online Resource
    Online Resource
    Effects of Different Irrigation Management on the Textural Properties of Double-Cropping Late Indica Rice in South China
    Computers, Materials and Continua (Tech Science Press) ; 2022
    In:  Phyton Vol. 91, No. 7 ( 2022), p. 1495-1502
    Details
    In: Phyton, Computers, Materials and Continua (Tech Science Press), Vol. 91, No. 7 ( 2022), p. 1495-1502
    Type of Medium: Online Resource
    ISSN: 1851-5657
    URL: Article
    DOI: 10.32604/phyton.2022.018485
    Language: English
    Publisher: Computers, Materials and Continua (Tech Science Press)
    Publication Date: 2022
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  • 10
    Online Resource
    Online Resource
    Grain yield improvement in high-quality rice varieties released in southern China from 2007 to 2017
    Frontiers Media SA ; 2022
    In:  Frontiers in Sustainable Food Systems Vol. 6 ( 2022-10-12)
    Details
    In: Frontiers in Sustainable Food Systems, Frontiers Media SA, Vol. 6 ( 2022-10-12)
    Abstract: In recent years, high-quality rice varieties have been widely cultivated for food production in southern China. However, changes in the yield performance of different high-quality rice varieties are still unclear. In this study, the yield and yield components of 710 different types (hybrid or inbred rice and japonica or indica ) of high-quality rice varieties were investigated in six provinces from 2007 to 2017. The results showed that, from 2007 to 2017, the grain yield and yield traits, including the number of spikelets per panicle and seed-set percentage, of high-quality indica rice varieties increased significantly, while the number of panicles decreased only in indica inbred rice. The grain yield of high-quality japonica rice also increased significantly, whereas japonica hybrid rice increased the number of spikelets per panicle and decreased the number of panicles. Compared with inbred rice, hybrid rice had a significant increase in grain yield due to a higher number of spikelets, rather than a lower number of panicles and seed-set percentage. Meanwhile, japonica rice showed higher grain yield than indica rice, which was attributed to seed-set percentage and an optimized structure between the number of panicles and the number of spikelets. In addition, the coefficient of variation of the grain yield of japonica rice decreased, whereas that of indica rice increased over time, and those of the number of panicles and seed-set percentage remained stable. Among the six provinces, Zhejiang had the highest grain yield because the number of spikelets per panicle and seed-set percentage increased over time. Our results suggested that, based on an increase in the yield potential of high-quality rice varieties over the past 11 years, future breeding of high-quality rice should be emphasized to improve the number of panicles and seed-set percentage for hybrid rice and the number of spikelets for inbred rice, especially the grain weight for indica inbred rice.
    Type of Medium: Online Resource
    ISSN: 2571-581X
    URL: Article
    DOI: 10.3389/fsufs.2022.986655
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2928540-9
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