In:
Digestive Surgery, S. Karger AG, Vol. 20, No. 3 ( 2003), p. 238-245
Abstract:
〈 i 〉 Background/Aims: 〈 /i 〉 This study assessed the effects of intermittent or continuous hepatic ischaemia and reperfusion with or without dimethyl sulphoxide (DMSO) pre-treatment in a rat ischaemic model. 〈 i 〉 Methods: 〈 /i 〉 One hundred and eighty rats were divided into three groups undergoing hepatic ischaemia of a total duration of 60, 90 and 120 min. Each group of rats was subdivided to receive either a continuous Pringle manoeuvre or intermittent liver pedicle clamping of 30 or 15 min. Ten minutes before ischaemia induction, 10 rats from each group were pre-treated with DMSO (500 mg/kg, b.w.) intravenously. 〈 i 〉 Results: 〈 /i 〉 With continuous hepatic pedicle clamping, survival rates inversely correlated with the duration of ischaemia, with greater survival in the intermittently clamped groups (p 〈 0.05). DMSO pre-treatment did not affect survival but resulted in a significant reduction in liver enzyme (aspartate aminotransferase, alanine aminotransferase) release on the first postoperative day following total ischaemic times of 90 min or greater (p 〈 0.05). After 120 min of total ischaemia, DMSO pre-treatment resulted in higher preservation of adenosine 5′-triphosphate liver content (p 〈 0.05). 〈 i 〉 Conclusion: 〈 /i 〉 DMSO may be used to prolong tolerance to inflow occlusion and to limit the adverse effects of ischaemia and reperfusion cycles in an experimental hepatic ischaemia model.
Type of Medium:
Online Resource
ISSN:
0253-4886
,
1421-9883
Language:
English
Publisher:
S. Karger AG
Publication Date:
2003
detail.hit.zdb_id:
1468560-7
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