In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 57, No. 12 ( 2007-12-01), p. 2964-2969
Abstract:
A thermophilic, methylotrophic methanogen, strain ZC-1 T , was isolated from the Shengli oilfield, China. Cells of strain ZC-1 T were motile cocci, 0.7–1.0 μm in diameter and always occurred in clusters of two to four cells. Lysis-susceptibility experiments and analysis of transmission electron micrographs of strain ZC-1 T suggested the presence of a proteinaceous cell wall. Strain ZC-1 T used methanol, methylamine and trimethylamine as substrates for methanogenesis. Optimal growth, with a doubling time of around 5 h, occurred at pH 6.0–6.5, 65 °C, 0.3–0.5 M NaCl and 0.05–0.20 M MgCl 2 . The DNA G+C content of this organism was 56 mol%. Analysis of 16S rRNA gene sequence and the inferred amino acid sequence of the mcrA gene of strain ZC-1 T indicated that it is related specifically to members of the family Methanosaetaceae (90.6 and 76.6 % sequence similarity, respectively). However, strain ZC-1 T failed to grow with acetate as substrate for methanogenesis, which is a special characteristic of the family Methanosaetaceae . Based on these phenotypic and phylogenic characteristics, strain ZC-1 T is proposed to represent a novel genus and species, for which the name Methermicoccus shengliensis gen. nov., sp. nov. is proposed. The type strain is ZC-1 T (=CGMCC 1.5056 T =DSM 18856 T ). Methermicoccaceae fam. nov. is also proposed.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.65049-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2007
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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