In:
Applied and Environmental Microbiology, American Society for Microbiology, Vol. 45, No. 3 ( 1983-03), p. 884-891
Abstract:
Thirty-three bacterial strains were isolated from soil, utilizing optically asymmetric degradation of dl -2-hydroxy-4-methylpentanoic acid ( dl -HMPA) as the screening probe. Those strains were distributed in the following group and genera: Coryneform and Bacillus, Pseudomonas, and Streptomyces. Among them, the most potent strains, Bacillus freudenreichii NRS-137KH20B and Brevibacterium albidum NRS-130KH20B, could perform the resolution of more than 30 g of dl -HMPA per liter within 4 to 5 days of fermentation. Optically pure l - and d -HMPA enantiomers were obtained in more than 80% theoretical yield, whereas the transformed enantiomer was almost quantitatively recovered as 2-oxo-4-methyl-pentanoic acid in the culture broth. The enantiospecific dehydrogenation responsible for this resolution reaction had a rather wide substrate specificity on straight or branched aliphatic C 4 to C 16 2-hydroxy acids, exhibiting the optima at chain lengths of either C 7 or C 5 , although the enantiospecificity was not changed by chain length. The process was thus successfully extended to the preparation of optically pure C 5 to C 9 2-hydroxy acids.
Type of Medium:
Online Resource
ISSN:
0099-2240
,
1098-5336
DOI:
10.1128/aem.45.3.884-891.1983
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
1983
detail.hit.zdb_id:
223011-2
detail.hit.zdb_id:
1478346-0
SSG:
12
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