In:
Chemical Communications, Royal Society of Chemistry (RSC), Vol. 59, No. 38 ( 2023), p. 5709-5712
Abstract:
Multiple elements in human sperm have been demonstrated to play significant roles in the reproductive process, but their simultaneous detection in single cells remains challenging. We propose a novel analytical procedure using single-cell inductively coupled plasma-time of flight-mass spectrometry (scICP-TOF-MS) to simultaneously quantify multiple elements of individual sperm cells. A promising label-free cell identification strategy based on the endogenous element was developed to obtain valid data. The element contents exhibited varied degrees of heterogeneity in single cells. Machine learning-based analysis of the multi-dimension dataset indicated different distribution patterns and physiological roles among the simultaneously detected elements.
Type of Medium:
Online Resource
ISSN:
1359-7345
,
1364-548X
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2023
detail.hit.zdb_id:
1472881-3
Permalink