In:
American Journal of Physiology-Heart and Circulatory Physiology, American Physiological Society, Vol. 292, No. 2 ( 2007-02), p. H912-H920
Abstract:
Stimulation of local renin-angiotensin system and increased levels of oxidants characterize the diabetic heart. Downregulation of ANG II type 1 receptors (AT 1 ) and enhancement in PKC activity in the heart point out the role of AT 1 blockers in diabetes. The purpose of this study was to evaluate a potential role of an AT 1 blocker, candesartan, on abnormal Ca 2+ release mechanisms and its relationship with PKC in the cardiomyocytes from streptozotocin-induced diabetic rats. Cardiomyocytes were isolated enzymatically and then incubated with either candesartan or a nonspecific PKC inhibitor bisindolylmaleimide I (BIM) for 6–8 h at 37°C. Both candesartan and BIM applied on diabetic cardiomyocytes significantly restored the altered kinetic parameters of Ca 2+ transients, as well as depressed Ca 2+ loading of sarcoplasmic reticulum, basal Ca 2+ level, and spatiotemporal properties of the Ca 2+ sparks. In addition, candesartan and BIM significantly antagonized the hyperphosphorylation of cardiac ryanodine receptor (RyR2) and restored the depleted protein levels of both RyR2 and FK506 binding protein 12.6 (FKBP12.6). Furthermore, candesartan and BIM also reduced the increased PKC levels and oxidized protein thiol level in membrane fraction of diabetic rat cardiomyocytes. Taken together, these data demonstrate that AT 1 receptor blockade protects cardiomyocytes from development of cellular alterations typically associated with Ca 2+ release mechanisms in diabetes mellitus. Prevention of these alterations by candesartan may present a useful pharmacological strategy for the treatment of diabetic cardiomyopathy.
Type of Medium:
Online Resource
ISSN:
0363-6135
,
1522-1539
DOI:
10.1152/ajpheart.00824.2006
Language:
English
Publisher:
American Physiological Society
Publication Date:
2007
detail.hit.zdb_id:
1477308-9
SSG:
12
Permalink