In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 6 ( 2021-6-25), p. e0253188-
Abstract:
ABCC multidrug resistance-associated proteins (ABCCs/MRPs), a subfamily of ABC transporters, are involved in multiple physiological processes. Although these proteins have been characterized in some plants, limited efforts have been made to address their possible roles in Rehmannia glutinosa , a medicinal plant. Here, we scanned R . glutinosa transcriptome sequences and identified 18 RgABCC genes by in silico analysis. Sequence alignment revealed that the RgABCCs were closely phylogenetically related and highly conserved with other plant ABCCs/MRPs. Subcellular localization revealed that most of the RgABCCs were deposited in vacuoles and a few in plasma membranes. Tissue-specific expression of the RgABCCs indicated significant specific accumulation patterns, implicating their roles in the respective tissues. Differential temporal expression patterns of the RgABCCs exhibited their potential roles during root development. Various abiotic stress and hormone treatment experiments indicated that some RgABCCs could be transcriptionally regulated in roots. Furthermore, the transcription of several RgABCCs in roots was strongly activated by cadmium (Cd), suggesting possible roles under heavy metal stresses. Functional analysis of RgABCC1 heterologous expression revealed that it may increase the tolerance to Cd in yeast, implying its Cd transport activity. Our study provides a detailed inventory and molecular characterization of the RgABCCs and valuable information for exploring their functions in R . glutinosa .
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0253188
DOI:
10.1371/journal.pone.0253188.g001
DOI:
10.1371/journal.pone.0253188.g002
DOI:
10.1371/journal.pone.0253188.g003
DOI:
10.1371/journal.pone.0253188.g004
DOI:
10.1371/journal.pone.0253188.g005
DOI:
10.1371/journal.pone.0253188.g006
DOI:
10.1371/journal.pone.0253188.g007
DOI:
10.1371/journal.pone.0253188.t001
DOI:
10.1371/journal.pone.0253188.s001
DOI:
10.1371/journal.pone.0253188.s002
DOI:
10.1371/journal.pone.0253188.s003
DOI:
10.1371/journal.pone.0253188.s004
DOI:
10.1371/journal.pone.0253188.s005
DOI:
10.1371/journal.pone.0253188.s006
DOI:
10.1371/journal.pone.0253188.s007
DOI:
10.1371/journal.pone.0253188.s008
DOI:
10.1371/journal.pone.0253188.s009
DOI:
10.1371/journal.pone.0253188.s010
DOI:
10.1371/journal.pone.0253188.s011
DOI:
10.1371/journal.pone.0253188.s012
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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