In:
The EMBO Journal, EMBO, Vol. 41, No. 15 ( 2022-08)
Abstract:
image Characterization of microtubules in cells via microtubule‐targeting agents is not ideal for assessing dynamics of specific microtubule populations. Here, a novel approach using an engineered microtubule‐severing enzyme allows spatiotemporal manipulation of microtubule disassembly triggered by either chemicals or illumination. A triple glutamine mutation in Spastin (dNSpastin3Q) reduces its association with microtubules. Engineered dNSpastin3Q acts as a microtubule‐severing enzyme in living cells. Recruitment of dNSpastin3Q to microtubules can be controlled by chemical‐ or light‐induced dimerization. Induced dimerization of dNSpastin3Q leads to acute disassembly of target microtubule subtypes.
Type of Medium:
Online Resource
ISSN:
0261-4189
,
1460-2075
DOI:
10.15252/embj.2021110472
Language:
English
Publisher:
EMBO
Publication Date:
2022
detail.hit.zdb_id:
1467419-1
detail.hit.zdb_id:
586044-1
SSG:
12
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