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  • 1
    In: Journal of Cellular Physiology, Wiley, Vol. 236, No. 11 ( 2021-11), p. 7464-7472
    Abstract: Most patients that resuscitate successfully from cardiac arrest (CA) suffer from poor neurological prognosis. DL‐3‐n‐butylphthalide (NBP) is known to have neuroprotective effects via multiple mechanisms. This study aimed to investigate whether NBP can decrease neurological impairment after CA. We studied the protective role of NBP in the hippocampus of a rat model of cardiac arrest induced by asphyxia. Thirty‐nine rats were divided randomly into sham, control, and NBP groups. Rats in control and NBP groups underwent cardiopulmonary resuscitation (CPR) 6 min after asphyxia. NBP or vehicle (saline) was administered intravenously 10 min after the return of spontaneous circulation (ROSC). Ultrastructure of hippocampal neurons was observed under transmission electron microscope. NBP treatment improved neurological function up to 72 h after CA. The ultrastructural lesion in mitochondria recovered in the NBP‐treated CA model. In conclusion, our study demonstrated multiple therapeutic benefits of NBP after CA.
    Type of Medium: Online Resource
    ISSN: 0021-9541 , 1097-4652
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 1478143-8
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    IOS Press ; 2023
    In:  Isokinetics and Exercise Science Vol. 31, No. 1 ( 2023-01-20), p. 7-17
    In: Isokinetics and Exercise Science, IOS Press, Vol. 31, No. 1 ( 2023-01-20), p. 7-17
    Abstract: BACKGROUND: The ability of the lower-extremity muscle activation directly affects the performance and in turn interacts with the loading conditions of the muscle itself. However, systematic information concerning the characteristics of lower-extremity muscle during landings is lacking. In particular, the landing height and shoes are also important factors based on the actual situation, which could further contribute to understanding the neuromuscular activity and how biochemical response of the body tissues to double-leg drop landings. OBJECTIVE: The study was to investigate the effects of landing tasks on the activation of lower-extremity muscles and explore the relationship among movement control, landing heights, shoe cushioning, and muscle activities. METHODS: Twelve male basketball players were recruited to perform drop jump (DJ) and passive landing (PL) from three heights (30, 45, and 60 cm) while wearing highly-cushioned basketball shoes (HC) and less-cushioned control shoes (LC). EMG electrodes were used to record the activities of the target muscles (rectus femoris, vastus lateralis, biceps femoris, tibialis anterior, and lateral gastrocnemius) during the landing tasks. RESULTS: Pre- and post-activation activity of the lower-extremity muscles significantly decreased during PL compared with those during DJ (p 〈 0.05). No significant shoe effects on the characteristics of muscle activation and coactivation during DJ movements were observed. However, the participants wearing LC showed significantly higher muscle post-activation (p 〈 0.05) at the three drop heights during PL compared with those wearing HC. Coactivation of the ankle muscles was higher in LC than in HC during 30-cm PL (p 〈 0.05). CONCLUSIONS: The activation patterns of lower-extremity muscles can be significantly influenced by landing types. Highly-cushioned basketball shoes would help reduce the risk of injuries by appropriately tuning the muscles during the PL.
    Type of Medium: Online Resource
    ISSN: 0959-3020 , 1878-5913
    Language: Unknown
    Publisher: IOS Press
    Publication Date: 2023
    detail.hit.zdb_id: 2029236-3
    SSG: 31
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  • 3
    Online Resource
    Online Resource
    Ovid Technologies (Wolters Kluwer Health) ; 2021
    In:  Emergency and Critical Care Medicine Vol. 1, No. 2 ( 2021-12-1), p. 64-69
    In: Emergency and Critical Care Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 1, No. 2 ( 2021-12-1), p. 64-69
    Abstract: We aimed to investigate the association between bystander cardiopulmonary resuscitation (CPR) and survival of patients with out-of-hospital cardiac arrests (OHCA) in Beijing. Methods: This observational study analyzed adult patients with OHCA treated by the Beijing emergency medical service (EMS) from January 2013 to December 2017. Data were collected in a Utstein style with a 1-year follow-up and a primary outcome of survival to hospital discharge. Secondary outcomes were return of spontaneous circulation (ROSC), survival to admission, favorable neurological outcome at hospital discharge, and survival and favorable neurological outcomes of up to 1 year. Results: A total of 5016 patients with OHCA from Beijing's urban area were recorded by EMS, wherein 765 patients (15.25%) underwent bystander CPR. The data were propensity score-matched forage, sex, location, witness, aetiology, initial rhythm, and call to EMS arrival to compare the difference between the occurrence and nonoccurrence of bystander CPR. The survival upon the discharge of patients who experienced bystander CPR was superior to that of patients who did not receive bystander CPR (3.7% vs 1.2%, respectively; P   〈  0.001). Moreover, patients with OHCA resuscitated with bystander CPR achieved better outcomes of ROSC, survival to admission, favorable neurological outcome at hospital discharge, survival and favorable neurological outcome after 1 year compared with those who were not resuscitated with bystander CPR. Conclusion: Survival and neurological outcome of patients who underwent bystander CPR was better than those who underwent nonbystander CPR in Beijing. However, the rate of bystander CPR was low.
    Type of Medium: Online Resource
    ISSN: 2693-860X
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2021
    detail.hit.zdb_id: 3123831-2
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  • 4
    In: Biomedical Chromatography, Wiley, Vol. 31, No. 6 ( 2017-06)
    Abstract: Dantonic pill, consisting of Salviae miltiorrhize , Panax notoginseng and Borneol , is a widely used compound Chinese medicine for preventing and treating ischemic cardiovascular diseases in China. In the present study, an original and sensitive method for simultaneous determination of tanshinol (i.e. danshensu), protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 in rat plasma by liquid chromatography–tandem mass spectrometry operated in positive/negative ion switching mode was established and validated. The lower limits of quantification for tanshinol, protocatechuic aldehyde, protocatechuic acid, notoginsenoside R1, ginsenoside Rg1 and Rb1 were 5, 0.5, 1, 0.5, 0.5 and 2 ng/mL, respectively. All of the calibration curves showed good linearity over the investigated concentration range ( r   〉  0.99). Validation results demonstrated that the above compounds were accurately, precisely and robustly quantified in rat plasma. The method was successfully applied to characterize the pharmacokinetic profiles of all six compounds in rats following a single oral administration of Dantonic pill.
    Type of Medium: Online Resource
    ISSN: 0269-3879 , 1099-0801
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 1479945-5
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Frontiers Media SA ; 2021
    In:  Frontiers in Pharmacology Vol. 11 ( 2021-1-19)
    In: Frontiers in Pharmacology, Frontiers Media SA, Vol. 11 ( 2021-1-19)
    Abstract: Aims: The biological functions of cyclin B1 (CCNB1) in colon adenocarcinoma (COAD) will be explored in this study. Furthermore, the therapeutic effects and potential molecular mechanisms of ursolic acid (UA) in COAD cells will also be investigated in vitro . Methods: COAD data were obtained from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Differentially expressed genes (DEGs) were determined with differential analysis. The biological functions of CCNB1 were analyzed through the GeneCards, the Search Tool for the Retrieval of Interacting Genes (STRING), and the Database for Annotation, Visualization, and Integrated Discovery (DAVID) databases. Therapeutic effects of UA on COAD cell lines HCT-116 and SW-480 were analyzed by CCK-8 and high-content screening (HCS) imaging assay. Flow cytometry was utilized to detect cell cycle changes of SW-480 and HCT-116 cells. Levels of mRNA and expression proteins of HCT-116, SW-480, and normal colon epithelial cells NCM-460 were determined by qRT-PCR and western blot. Results: CCNB1 was highly expressed and acted as an oncogene in COAD patients. CCNB1 and its interacting genes were significantly enriched in the cell cycle pathway. UA effectively inhibited the proliferation and injured COAD cells. In addition, UA arrested cell cycle of COAD cells in S phase. With regard to the molecular mechanisms of UA, we demonstrated that UA can significantly downregulate CCNB1 and its interacting genes and proteins, including CDK1, CDC20, CCND1, and CCNA2, which contributed to cell cycle blocking and COAD treatment. Conclusion: Results from this study revealed that UA possesses therapeutic effects on COAD. The anti-COAD activities of UA are tightly related to suppression of CCNB1 and its interacting targets, which is crucial in abnormal cell cycle process.
    Type of Medium: Online Resource
    ISSN: 1663-9812
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2021
    detail.hit.zdb_id: 2587355-6
    SSG: 15,3
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  • 6
    Online Resource
    Online Resource
    Informa UK Limited ; 2023
    In:  Clinical, Cosmetic and Investigational Dermatology Vol. Volume 16 ( 2023-07), p. 1741-1747
    In: Clinical, Cosmetic and Investigational Dermatology, Informa UK Limited, Vol. Volume 16 ( 2023-07), p. 1741-1747
    Type of Medium: Online Resource
    ISSN: 1178-7015
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2494852-4
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  • 7
    Online Resource
    Online Resource
    Informa UK Limited ; 2023
    In:  Clinical, Cosmetic and Investigational Dermatology Vol. Volume 16 ( 2023-05), p. 1319-1323
    In: Clinical, Cosmetic and Investigational Dermatology, Informa UK Limited, Vol. Volume 16 ( 2023-05), p. 1319-1323
    Type of Medium: Online Resource
    ISSN: 1178-7015
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2023
    detail.hit.zdb_id: 2494852-4
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  • 8
    In: Biomedical Chromatography, Wiley, Vol. 29, No. 4 ( 2015-04), p. 552-559
    Abstract: A simple, sensitive and selective high‐performance liquid chromatography electrospray ionization tandem mass spectrometry (LC‐MS/MS) method was developed for simultaneous determination and pharmacokinetic study of caffeic acid (CA) and its active metabolites. The separation with isocratic elution used a mobile phase composed of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. The detection of target compounds was done in selected reaction monitoring (SRM) mode. The SRM detection was operated in the negative electrospray ionization mode using the transitions m/z 179 ([M − H] − ) → 135 for CA, m/z 193 ([M − H] − ) → 134.8 for ferulic acid and isoferulic acid and m/z 153 ([M − H] − ) → 108 for protocatechuic acid. The method was linear for all analytes over the investigated range with all correlation coefficients 0.9931. The lower limits of quantification were 5.0 ng/mL for analytes. The intra‐ and inter‐day precisions (relative standard deviation) were 〈 5.86 and 〈 6.52%, and accuracy (relative error) was between −5.95 and 0.35% ( n =  6). The developed method was applied to study the pharmacokinetics of CA and its major active metabolites in rat plasma after oral and intravenous administration of CA. Copyright © 2014 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0269-3879 , 1099-0801
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 1479945-5
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Elsevier BV ; 2018
    In:  Colloids and Surfaces A: Physicochemical and Engineering Aspects Vol. 547 ( 2018-06), p. 8-18
    In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Elsevier BV, Vol. 547 ( 2018-06), p. 8-18
    Type of Medium: Online Resource
    ISSN: 0927-7757
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2018
    detail.hit.zdb_id: 1500517-3
    detail.hit.zdb_id: 1169792-1
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  • 10
    Online Resource
    Online Resource
    Elsevier BV ; 2015
    In:  Chinese Journal of Chemical Engineering Vol. 23, No. 4 ( 2015-04), p. 597-604
    In: Chinese Journal of Chemical Engineering, Elsevier BV, Vol. 23, No. 4 ( 2015-04), p. 597-604
    Type of Medium: Online Resource
    ISSN: 1004-9541
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2015
    detail.hit.zdb_id: 1480835-3
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