In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-8-22)
Abstract:
Four P4-ATPase flippase genes, VdDrs2, VdNeo1, VdP4-4 , and VdDnf1 were identified in Verticillium dahliae , one of the most devastating phytopathogenic fungi in the world. Knock out of VdDrs2, VdNeo1 , and VdP4-4 , or knock down of VdDnf1 significantly decreased the pathogenicity of the mutants in cotton. Among the mutants, the greatest decrease in pathogenicity was observed in Δ VdDrs2 . VdDrs2 was localized to plasma membrane, vacuoles, and trans -Golgi network (TGN). In vivo observation showed that the infection of the cotton by Δ VdDrs2 was significantly delayed. The amount of two known Verticillium toxins, sulfacetamide, and fumonisin B1 in the fermentation broth produced by the Δ VdDrs2 strain was significantly reduced, and the toxicity of the crude Verticillium wilt toxins to cotton cells was attenuated. In addition, the defect of VdDrs2 impaired the synthesis of melanin and the formation of microsclerotia, and decreased the sporulation of V. dahliae . Our data indicate a key role of P4 ATPases-associated vesicle transport in toxin secretion of disease fungi and support the importance of mycotoxins in the pathogenicity of V. dahliae .
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2022.944364
DOI:
10.3389/fpls.2022.944364.s001
DOI:
10.3389/fpls.2022.944364.s002
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2613694-6
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