In:
Development, Growth & Differentiation, Wiley, Vol. 55, No. 6 ( 2013-08), p. 635-647
Abstract:
In spermatogenesis, the G olgi apparatus is important for the formation of the acrosome, which is a sperm‐specific organelle essential for fertilization. Comprehensive examinations of the spatiotemporal distribution and morphological characterizations of the G olgi in various cells during spermatogenesis are necessary for functional analyses and mutant screenings in the model eukaryote D rosophila . Here, we examined the distribution and morphology of the G olgi during D rosophila spermatogenesis with immunofluorescence and electron microscopy. In pre‐meiotic germ cells, the G olgi apparatuses were distributed evenly in the cytoplasm. In contrast, they were located exclusively in two regions near the poles during the meiotic metaphase, where they were segregated prior to the chromosomes. In cells in anaphase to telophase, the G olgi were predominantly left behind in the equatorial region between the separating daughter nuclei. After completion of meiosis, the dispersed G olgi were assembled at the apical side of the spermatid nucleus to form the acrosome. Further investigation of the G olgi distribution in β2‐tubulin mutants showed aberrant and uneven distributions of the G olgi among sister cells in the meiotic spermatocytes and in the post‐meiotic spermatids. At the ultrastructural level, the G olgi apparatus in pre‐meiotic spermatocytes comprised a pair of stacks. The two stacks were situated adjacent to each other, as if they had duplicated before entering into meiotic division. These results highlight the dynamic nature of the G olgi during spermatogenesis and provide a framework for analyzing the correlations between the dynamics of the G olgi and its function in sperm development.
Type of Medium:
Online Resource
ISSN:
0012-1592
,
1440-169X
DOI:
10.1111/dgd.2013.55.issue-6
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2020067-5
SSG:
12
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