In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 9 ( 2021-9-10), p. e0256588-
Abstract:
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle infected with BLV and those without BLV. In BLV-infected cattle, BLV-derived miRNAs (blv-miRNAs) accounted for 38% of all miRNAs in B cells. Four of these blv-miRNAs (blv-miR-B1-5p, blv-miR-B2-5p, blv-miR-B4-3p, and blv-miR-B5-5p) had highly significant positive correlations with BLV proviral load (PVL). The read counts of 90 host-derived miRNAs (bta-miRNAs) were significantly down-regulated in BLV-infected cattle compared to those in uninfected cattle. Only bta-miR-375 had a positive correlation with PVL in BLV-infected cattle and was highly expressed in the B cell lymphoma tissue of EBL cattle. There were a few bta-miRNAs that correlated with BLV tax/rex gene expression; however, BLV AS1 expression had a significant negative correlation with many of the down-regulated bta-miRNAs that are important for tumor development and/or tumor suppression. These results suggest that BLV promotes lymphomagenesis via AS1 and blv-miRNAs, rather than tax/rex , by down-regulating the expression of bta-miRNAs that have a tumor-suppressing function, and this downregulation is linked to increased PVL.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0256588
DOI:
10.1371/journal.pone.0256588.g001
DOI:
10.1371/journal.pone.0256588.g002
DOI:
10.1371/journal.pone.0256588.g003
DOI:
10.1371/journal.pone.0256588.t001
DOI:
10.1371/journal.pone.0256588.t002
DOI:
10.1371/journal.pone.0256588.t003
DOI:
10.1371/journal.pone.0256588.t004
DOI:
10.1371/journal.pone.0256588.s001
DOI:
10.1371/journal.pone.0256588.s002
DOI:
10.1371/journal.pone.0256588.s003
DOI:
10.1371/journal.pone.0256588.s004
DOI:
10.1371/journal.pone.0256588.s005
DOI:
10.1371/journal.pone.0256588.s006
DOI:
10.1371/journal.pone.0256588.r001
DOI:
10.1371/journal.pone.0256588.r002
DOI:
10.1371/journal.pone.0256588.r003
DOI:
10.1371/journal.pone.0256588.r004
DOI:
10.1371/journal.pone.0256588.r005
DOI:
10.1371/journal.pone.0256588.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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