In:
The Journal of Immunology, The American Association of Immunologists, Vol. 69, No. 1 ( 1952-07-01), p. 13-25
Abstract:
By the monolayer technique the relationship between the number of haptenic groups in azoproteins and the number of antibody molelules combined was quantitatively observed. The optical thickness of the antibody layer specifically attached to a monolayer film of atoxyl-azo-ovalbumin increases in linear relation to the increase in the number of haptenic atoxyl groups, until it reaches a “saturation” point with an antigen containing about 30 haptenic groups, and thereafter the thickness remains unchanged, even if the number of determinant groups is increased over 30. Quantitative observations were made with monolayer films of mixtures of azoprotein (containing a certain number of haptenic groups) and native ovalbumin in various proportions. The thickness of the antibody layer decreased proportionally with the decrease in the average number of haptenic groups per unit area in the mixture films (hetero-monolayers), approximately corresponding to the thickness of the antibody layer obtained with homogeneous film (homomonolayer) containing the same number of haptenic groups per molecule. In multilayer films the thickness of the antibody layer increased proportionally with the number of layers each containing the same number of haptenic groups. The ratio of increase in the thickness of the antibody layer is in complete accord with that in the case of a single monolayer experiment. From the experimental results described in this paper the significance of the thickness of the antibody layer in a monolayer technique was made clear in that it indicates the average density of antibodies adsorbed on an antigen monolayer film. The structural arrangements of the haptenic groups in the monolayer film and of the antibodies on the antigen film were discussed in terms of molecular orientation.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.69.1.13
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
1952
detail.hit.zdb_id:
1475085-5
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