In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 11 ( 2022-11-21), p. e0277701-
Abstract:
A symbiotic relationship is observed between Armillaria and the Chinese herbal medicine Gastrodia elata ( G . elata ). Armillaria is a nutrient source for the growth of G . elata , and its nutrient metabolism efficiency affects the growth and development of G . elata . Auxin has been reported to stimulate Armillaria species, but the molecular mechanism remains unknown. We found that naphthalene acetic acid (NAA) can also promote the growth of A . gallica . Moreover, we identified a total of 2071 differentially expressed genes (DEGs) by analyzing the transcriptome sequencing data of A . gallica at 5 and 10 hour of NAA treatment. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these unigenes were significantly enriched in the metabolism pathways of arginine, proline, propanoate, phenylalanine and tryptophan. The expression levels of the general amino acid permease (Gap), ammonium transporter (AMT), glutamate dehydrogenase (GDH), glutamine synthetase (GS), Zn(II) 2Cys6 and C2H2 transcription factor genes were upregulated. Our transcriptome analysis showed that the amino acid and nitrogen metabolism pathways in Armillaria were rapidly induced within hours after NAA treatment. These results provide valuable insights into the molecular mechanisms by which NAA promotes the growth of Armillaria species.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0277701
DOI:
10.1371/journal.pone.0277701.g001
DOI:
10.1371/journal.pone.0277701.g002
DOI:
10.1371/journal.pone.0277701.g003
DOI:
10.1371/journal.pone.0277701.g004
DOI:
10.1371/journal.pone.0277701.g005
DOI:
10.1371/journal.pone.0277701.g006
DOI:
10.1371/journal.pone.0277701.t001
DOI:
10.1371/journal.pone.0277701.s001
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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