In:
PLOS Biology, Public Library of Science (PLoS), Vol. 20, No. 1 ( 2022-1-26), p. e3001456-
Abstract:
In traumatic brain injury (TBI), the initial injury phase is followed by a secondary phase that contributes to neurodegeneration, yet the mechanisms leading to neuropathology in vivo remain to be elucidated. To address this question, we developed a Drosophila head-specific model for TBI termed Drosophila Closed Head Injury (dCHI), where well-controlled, nonpenetrating strikes are delivered to the head of unanesthetized flies. This assay recapitulates many TBI phenotypes, including increased mortality, impaired motor control, fragmented sleep, and increased neuronal cell death. TBI results in significant changes in the transcriptome, including up-regulation of genes encoding antimicrobial peptides (AMPs). To test the in vivo functional role of these changes, we examined TBI-dependent behavior and lethality in mutants of the master immune regulator NF-κB, important for AMP induction, and found that while sleep and motor function effects were reduced, lethality effects were enhanced. Similarly, loss of most AMP classes also renders flies susceptible to lethal TBI effects. These studies validate a new Drosophila TBI model and identify immune pathways as in vivo mediators of TBI effects.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001456
DOI:
10.1371/journal.pbio.3001456.g001
DOI:
10.1371/journal.pbio.3001456.g002
DOI:
10.1371/journal.pbio.3001456.g003
DOI:
10.1371/journal.pbio.3001456.g004
DOI:
10.1371/journal.pbio.3001456.g005
DOI:
10.1371/journal.pbio.3001456.g006
DOI:
10.1371/journal.pbio.3001456.g007
DOI:
10.1371/journal.pbio.3001456.s001
DOI:
10.1371/journal.pbio.3001456.s002
DOI:
10.1371/journal.pbio.3001456.s003
DOI:
10.1371/journal.pbio.3001456.s004
DOI:
10.1371/journal.pbio.3001456.s005
DOI:
10.1371/journal.pbio.3001456.s006
DOI:
10.1371/journal.pbio.3001456.s007
DOI:
10.1371/journal.pbio.3001456.s008
DOI:
10.1371/journal.pbio.3001456.s009
DOI:
10.1371/journal.pbio.3001456.s010
DOI:
10.1371/journal.pbio.3001456.s011
DOI:
10.1371/journal.pbio.3001456.s012
DOI:
10.1371/journal.pbio.3001456.s013
DOI:
10.1371/journal.pbio.3001456.s014
DOI:
10.1371/journal.pbio.3001456.s015
DOI:
10.1371/journal.pbio.3001456.s016
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10.1371/journal.pbio.3001456.s017
DOI:
10.1371/journal.pbio.3001456.s018
DOI:
10.1371/journal.pbio.3001456.s019
DOI:
10.1371/journal.pbio.3001456.s020
DOI:
10.1371/journal.pbio.3001456.s021
DOI:
10.1371/journal.pbio.3001456.s022
DOI:
10.1371/journal.pbio.3001456.s023
DOI:
10.1371/journal.pbio.3001456.s024
DOI:
10.1371/journal.pbio.3001456.r001
DOI:
10.1371/journal.pbio.3001456.r002
DOI:
10.1371/journal.pbio.3001456.r003
DOI:
10.1371/journal.pbio.3001456.r004
DOI:
10.1371/journal.pbio.3001456.r005
DOI:
10.1371/journal.pbio.3001456.r006
DOI:
10.1371/journal.pbio.3001456.r007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2126773-X
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