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  • 1
    Online Resource
    Online Resource
    Peptidome‐wide association analysis of Epstein−Barr virus identifies epitope repertoires associated with nasopharyngeal carcinoma
    Wiley ; 2023
    In:  Journal of Medical Virology Vol. 95, No. 6 ( 2023-06)
    Details
    In: Journal of Medical Virology, Wiley, Vol. 95, No. 6 ( 2023-06)
    Abstract: Human leukocyte antigen (HLA) molecules are essential for presenting Epstein−Barr virus (EBV) antigens and are closely related to nasopharyngeal carcinoma (NPC). This study aims to systematically investigate the association between HLA‐bound EBV peptides and NPC risk through in silico HLA‐peptide binding prediction. A total of 455 NPC patients and 463 healthy individuals in NPC endemic areas were recruited, and HLA‐target sequencing was performed. HLA‐peptide binding prediction for EBV, followed by peptidome‐wide logistic regression and motif analysis, was applied. Binding affinity changes for EBV peptides carrying high‐risk mutations were analyzed. We found that NPC‐associated EBV peptides were significantly enriched in immunogenic proteins and core linkage disequilibrium (LD) proteins related to evolution, especially those binding HLA‐A alleles ( p  = 3.10 × 10 −4 for immunogenic proteins and p  = 8.10 × 10 −5 for core LD proteins related to evolution). These peptides were clustered and showed binding motifs of HLA supertypes, among which supertype A02 presented an NPC‐risk effect ( p adj  = 3.77 × 10 −4 ) and supertype A03 presented an NPC‐protective effect ( p adj  = 4.89 × 10 −4 ). Moreover, a decreased binding affinity toward risk HLA supertype A02 was observed for the peptide carrying the NPC‐risk mutation BNRF1 V1222I ( p  = 0.0078), and an increased binding affinity toward protective HLA supertype A03 was observed for the peptide carrying the NPC‐risk mutation BALF2 I613V ( p  = 0.022). This study revealed the distinct preference of EBV peptides for binding HLA supertypes, which may contribute to shaping EBV population structure and be involved in NPC development.
    Type of Medium: Online Resource
    ISSN: 0146-6615 , 1096-9071
    URL: Issue
    URL: Article
    DOI: 10.1002/jmv.v95.6
    DOI: 10.1002/jmv.28860
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 752392-0
    detail.hit.zdb_id: 1475090-9
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  • 2
    Online Resource
    Online Resource
    Copper catalyzed borylative cyclization of 3-arylallyl carbamoyl chloride with B2pin2: stereoselective synthesis of cis-2-aryl-3-boryl-γ-lactams
    Elsevier BV ; 2021
    In:  Chinese Chemical Letters Vol. 32, No. 7 ( 2021-07), p. 2297-2300
    Details
    In: Chinese Chemical Letters, Elsevier BV, Vol. 32, No. 7 ( 2021-07), p. 2297-2300
    Type of Medium: Online Resource
    ISSN: 1001-8417
    URL: Article
    DOI: 10.1016/j.cclet.2021.02.016
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2096242-3
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  • 3
    Online Resource
    Online Resource
    A novel nonsense variant of the AGXT identified in a Chinese family: special variant research in the Chinese reference genome
    Springer Science and Business Media LLC ; 2021
    In:  BMC Nephrology Vol. 22, No. 1 ( 2021-12)
    Details
    In: BMC Nephrology, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2021-12)
    Abstract: Primary hyperoxaluria(PH)is a rare autosomal recessive genetic disease that contains three subtypes (PH1, PH2 and PH3). Approximately 80% of PH patients has been reported as subtype PH1, this subtype of PH has been related to a higher risk of renal failure at any age. Several genetic studies indicate that the variants in gene AGXT are responsible for the occurrence of PH1. However, the population heterogeneity of the variants in AGXT makes the genetic diagnosis of PH1 more challenging as it is hard to locate each specific variant. It is valuable to have a complete spectrum of AGXT variants from different population for early diagnosis and clinical treatments of PH1. Case presentation In this study, We performed high-throughput sequencing and genetic analysis of a 6-year-old male PH1 patient from a Chinese family. Two variants (c.346G  〉  A: p.Gly116Arg; c.864G  〉  A: p.Trp288X) of the gene AGXT were identified. We found a nonsense variant (c.864G  〉  A: p.Trp288X) that comes from the proband’s mother and has never been reported previously. The other missense variant (c.346G  〉  A: p.Gly116Arg) was inherited from his father and has been found previously in a domain of aminotransferase, which plays an important role in the function of AGT protein. Furthermore, we searched 110 pathogenic variants of AGXT that have been reported worldwide in healthy local Chinese population, none of these pathogenic variants was detected in the local genomes. Conclusions Our research provides an important diagnosis basis for PH1 on the genetic level by updating the genotype of PH1 and also develops a better understanding of the variants in AGXT by broadening the variation database of AGXT according to the Chinese reference genome.
    Type of Medium: Online Resource
    ISSN: 1471-2369
    URL: Article
    DOI: 10.1186/s12882-021-02276-3
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2041348-8
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  • 4
    Online Resource
    Online Resource
    Identification of a potent MAR element from the human genome and assessment of its activity in stably transfected CHO cells
    Wiley ; 2018
    In:  Journal of Cellular and Molecular Medicine Vol. 22, No. 2 ( 2018-02), p. 1095-1102
    Details
    In: Journal of Cellular and Molecular Medicine, Wiley, Vol. 22, No. 2 ( 2018-02), p. 1095-1102
    Abstract: Low‐level and unstable transgene expression are common issues using the CHO cell expression system. Matrix attachment regions (MARs) enhance transgene expression levels, but additional research is needed to improve their function and to determine their mechanism of action. MAR‐6 from CHO chromosomes actively mediates high and consistent gene expression. In this study, we compared the effects of two new MARs and MAR‐6 on transgene expression in recombinant CHO cells and found one potent MAR element that can significantly increase transgene expression. Two MARs, including the human CSP‐B MAR element and DHFR intron MAR element from CHO cells, were cloned and inserted downstream of the poly(A) site in a eukaryotic vector. The constructs were transfected into CHO cells, and the expression levels and stability of eGFP were detected by flow cytometry. The three MAR sequences can be ranked in terms of overall eGFP expression, in decreasing order, as follows: human CSP‐B, DHFR intron MAR element and MAR‐6. Additionally, as expected, the three MAR‐containing vectors showed higher transfection efficiencies and transient transgene expression in comparison with those of the non‐MAR‐containing vector. Bioinformatics analysis indicated that the NFAT and VIBP elements within MAR sequences may contribute to the enhancement of eGFP expression. In conclusion, the human CSP‐B MAR element can improve transgene expression and its effects may be related to the NFAT and VIBP elements.
    Type of Medium: Online Resource
    ISSN: 1582-1838 , 1582-4934
    URL: Issue
    URL: Article
    DOI: 10.1111/jcmm.2018.22.issue-2
    DOI: 10.1111/jcmm.13361
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2076114-4
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  • 5
    Online Resource
    Online Resource
    Determination of Phthalate Esters in Plastic Track Surface Layer Material by Gas Chromatography
    Trans Tech Publications, Ltd. ; 2017
    In:  Key Engineering Materials Vol. 726 ( 2017-1), p. 43-49
    Details
    In: Key Engineering Materials, Trans Tech Publications, Ltd., Vol. 726 ( 2017-1), p. 43-49
    Abstract: A method was proposed to simultaneously and rapidly determine the content of five kinds of phthalate esters (DMP, DBP, BBP, DnHP, DOP) in plastic track surface layer material by gas chromatography (GC) with the external standard,and the method was first proposed. The sample pretreatment method was microwave-assisted extracted using ethyl acetate as the solvent. Discussed the effects of extraction conditions on the extraction efficiency, microwave extraction time was 30 minutes, microwave extraction temperature was 100°C,the volume of ethyl acetate was 15mL. The results showed that there was a good linear relationship in range of 0.040 to 50 mg/L(R 2 was 0.9987-0.9994). The average recoveries of standard addition for five kinds of phthalate esters were between 83.83% and 101.02%, and the relative standard derivations (RSDs (n=6)) ranged from 0.95% to 4.73%. The three times of signal to noise ratio (3 S/N) was used to calculate the detection limit of the method , and the detection limit was 2.5-3.0 mg/kg. The ten times of signal to noise ratio (10 S/N) was used to calculate the quantitation limit of the method, and the limit of quantitation was 8.5-10 mg/kg. This method is simple, effective and suitable for the determination of phthalate esters in plastic track surface layer material.
    Type of Medium: Online Resource
    ISSN: 1662-9795
    URL: Issue
    URL: Article
    DOI: 10.4028/www.scientific.net/KEM.726
    DOI: 10.4028/www.scientific.net/KEM.726.43
    Language: Unknown
    Publisher: Trans Tech Publications, Ltd.
    Publication Date: 2017
    detail.hit.zdb_id: 2073306-9
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  • 6
    Online Resource
    Online Resource
    Effects of Sintering Temperature on Phases, Microstructures and Properties of Fused Silica Ceramics
    Trans Tech Publications, Ltd. ; 2017
    In:  Key Engineering Materials Vol. 726 ( 2017-1), p. 399-403
    Details
    In: Key Engineering Materials, Trans Tech Publications, Ltd., Vol. 726 ( 2017-1), p. 399-403
    Abstract: Fused silica ceramics, named fused silica articles by means of ceramics fabrication processes, had been widely used in metallurgy, refractory, aviation and many other areas. How to get fused silica ceramics with high density and less cristobalite is on the focus. In this paper, effects of sintering temperature (1150°C, 1200°C, 1250°C, 1300°C, 1350°C) on phases, microstructures and properties of fused silica ceramics were investigated. The results showed that the bulk density of samples increased and the apparent porosity of samples minished gradually, with the increase of sintering temperature. However, the bending strength increased to maximum firstly and then decreased because the emergency of cristobalite at 1300°C. When the sintering temperature was 1250°C, the bulk density, apparent porosity and bending strength was 1.71g·cm -3 , 17.6% and 38.87MPa, respectively.
    Type of Medium: Online Resource
    ISSN: 1662-9795
    URL: Issue
    URL: Article
    DOI: 10.4028/www.scientific.net/KEM.726
    DOI: 10.4028/www.scientific.net/KEM.726.399
    Language: Unknown
    Publisher: Trans Tech Publications, Ltd.
    Publication Date: 2017
    detail.hit.zdb_id: 2073306-9
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  • 7
    Online Resource
    Online Resource
    High expression of Anxa2 and Stat3 promote progression of hepatocellular carcinoma and predict poor prognosis
    Elsevier BV ; 2019
    In:  Pathology - Research and Practice Vol. 215, No. 6 ( 2019-06), p. 152386-
    Details
    In: Pathology - Research and Practice, Elsevier BV, Vol. 215, No. 6 ( 2019-06), p. 152386-
    Type of Medium: Online Resource
    ISSN: 0344-0338
    URL: Article
    DOI: 10.1016/j.prp.2019.03.015
    RVK:
    XA 10000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2019
    detail.hit.zdb_id: 2039756-2
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  • 8
    Online Resource
    Online Resource
    Shortened nuclear matrix attachment regions are sufficient for replication and maintenance of episomes in mammalian cells
    American Society for Cell Biology (ASCB) ; 2019
    In:  Molecular Biology of the Cell Vol. 30, No. 22 ( 2019-10-15), p. 2761-2770
    Details
    In: Molecular Biology of the Cell, American Society for Cell Biology (ASCB), Vol. 30, No. 22 ( 2019-10-15), p. 2761-2770
    Abstract: Matrix attachment regions (MARs) can mediate the replication of vector episomes in mammalian cells; however, the molecular mode of action remains unclear. Here, we assessed the characteristics of MARs and the mechanism that mediates episomal vector replication in mammalian cells. Five shortened subfragments of β-interferon MAR fragments were cloned and transferred into CHO cells, and transgene expression levels, presence of the gene, and the episomal maintenance mechanism were determined. Three shortened MAR derivatives (position 781–1320, 1201–1740, and 1621–2201) retained full MAR activity and mediated episomal vector replication. Moreover, the three shortened MARs showed higher transgene expression levels, greater efficiency in colony formation, and more persistent transgene expression compared with those of the original pEPI-1 plasmid, and three functional truncated MARs can bind to SAF-A MAR-binding protein. These results suggest that shortened MARs are sufficient for replication and maintenance of episomes in CHO cells.
    Type of Medium: Online Resource
    ISSN: 1059-1524 , 1939-4586
    URL: Article
    DOI: 10.1091/mbc.E19-02-0108
    Language: English
    Publisher: American Society for Cell Biology (ASCB)
    Publication Date: 2019
    detail.hit.zdb_id: 1474922-1
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Enhancing expression level and stability of transgene mediated by episomal vector via buffering DNA methyltransferase in transfected CHO cells
    Wiley ; 2019
    In:  Journal of Cellular Biochemistry Vol. 120, No. 9 ( 2019-09), p. 15661-15670
    Details
    In: Journal of Cellular Biochemistry, Wiley, Vol. 120, No. 9 ( 2019-09), p. 15661-15670
    Abstract: Nonviral episomal vectors present attractive alternative vehicles for gene therapy applications. Previously, we have established a new type of nonviral episomal vector‐mediated by the characteristic motifs of matrix attachment regions (MARs), which is driven by the cytomegalovirus (CMV) promoter. However, the CMV promoter is intrinsically susceptible to silencing, resulting in declined productivity during long‐term culture. In this study, Chinese hamster ovary (CHO) cells and DNA methyltransferase‐deficient (Dnmt3a‐deficient) CHO cells were transfected with plasmid‐mediated by MAR, or CHO cells were treated with the DNA methylation inhibitor 5‐Aza‐2′‐deoxycytidine. Flow cytometry, plasmid rescue experiments, fluorescence in‐situ hybridization (FISH), and bisulfite sequencing were performed to observe transgene expression, its state of existence, and the CpG methylation level of the CMV promoter. The results indicated that all DNA methylation inhibitor and methyltransferase deficient cells could increase transgene expression levels and stability in the presence or absence of selection pressure after a 60‐generation culture. Plasmid rescue assay and FISH analysis showed that the vector still existed episomally after long‐time culture. Moreover, a relatively lower CMV promoter methylation level was observed in Dnmt3a‐deficient cell lines and CHO cells treated with 5‐Aza‐2′‐deoxycytidine. In addition, Dnmt3a‐deficient cells were superior to the DNA methylation inhibitor treatment regarding the transgene expression and long‐term stability. Our study provides the first evidence that lower DNA methyltransferase can enhance expression level and stability of transgenes mediated by episomal vectors in transfected CHO cells.
    Type of Medium: Online Resource
    ISSN: 0730-2312 , 1097-4644
    URL: Issue
    URL: Article
    DOI: 10.1002/jcb.v120.9
    DOI: 10.1002/jcb.28835
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1479976-5
    SSG: 12
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    Online Resource
  • 10
    Online Resource
    Online Resource
    Keratin17 Promotes Tumor Growth and is Associated with Poor Prognosis in Gastric Cancer
    Ivyspring International Publisher ; 2018
    In:  Journal of Cancer Vol. 9, No. 2 ( 2018), p. 346-357
    Details
    In: Journal of Cancer, Ivyspring International Publisher, Vol. 9, No. 2 ( 2018), p. 346-357
    Type of Medium: Online Resource
    ISSN: 1837-9664
    URL: Article
    DOI: 10.7150/jca.19838
    Language: English
    Publisher: Ivyspring International Publisher
    Publication Date: 2018
    detail.hit.zdb_id: 2573318-7
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