In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 14 ( 2023-7-20)
Abstract:
Terpenes are the main class of secondary metabolites produced in response to pest and germ attacks. In maize ( Zea mays L.), they are the essential components of the herbivore-induced plant volatile mixture, which functioned as a direct or indirect defense against pest and germ attacks. In this study, 43 maize terpene synthase gene ( ZmTPS ) family members were systematically identified and analyzed through the whole genomes of maize. Nine genes, including Zm00001d032230 , Zm00001d045054 , Zm00001d024486 , Zm00001d004279 , Zm00001d002351 , Zm00001d002350 , Zm00001d053916 , Zm00001d015053 , and Zm00001d015054 , were isolated for their differential expression pattern in leaves after corn borer ( Ostrinia nubilalis ) bite. Additionally, six genes ( Zm00001d045054 , Zm00001d024486 , Zm00001d002351 , Zm00001d002350 , Zm00001d015053 , and Zm00001d015054 ) were significantly upregulated in response to corn borer bite. Among them, Zm00001d045054 was cloned. Heterologous expression and enzyme activity assays revealed that Zm00001d045054 functioned as d -limonene synthase. It was renamed ZmDLS . Further analysis demonstrated that its expression was upregulated in response to corn borer bites and Fusarium graminearum attacks. The mutant of ZmDLS downregulated the expressions of Zm00001d024486 , Zm00001d002351 , Zm00001d002350 , Zm00001d015053 , and Zm00001d015054 . It was more attractive to corn borer bites and more susceptible to F. graminearum infection. The yeast one-hybrid assay and dual-luciferase assay showed that ZmMYB76 and ZmMYB101 could upregulate the expression of ZmDLS by binding to the promoter region. This study may provide a theoretical basis for the functional analysis and transcriptional regulation of terpene synthase genes in crops.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2023.1162826
DOI:
10.3389/fpls.2023.1162826.s001
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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