In:
Chirality, Wiley, Vol. 25, No. 9 ( 2013-09), p. 487-492
Abstract:
Twelve chiral compounds were enantiomerically resolved on bovine serum albumin chiral stationary phase (BSA‐CSP) by high‐performance liquid chromatography (HPLC) in reversed‐phase modes. Chromatographic conditions such as mobile phase pH, the percentage of organic modifier, and concentration of analyte were optimized for separation of enantiomers. For N‐(2, 4‐dinitrophenyl)‐serine (DNP‐ser), the retention factors ( k ) greatly increase from 0.81 to 6.23 as the pH decreasing from 7.21 to 5.14, and the resolution factor ( R s ) exhibited a similar increasing trend (from 0 to 1.34). More interestingly, the retention factors for N‐(2, 4‐dinitrophenyl)‐proline (DNP‐pro) decrease along with increasing 1‐propanol in mobile phase (3%, 5%, 7% and 9% by volume), whereas the resolution factor shows an upward trend (from 0.96 to 2.04). Moreover, chiral recognition mechanisms for chiral analytes were further investigated through thermodynamic methods. Chirality 25:487–492, 2013 . © 2013 Wiley Periodicals, Inc.
Type of Medium:
Online Resource
ISSN:
0899-0042
,
1520-636X
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2001237-8
SSG:
12
SSG:
15,3
Permalink