In:
Genome, Canadian Science Publishing, Vol. 49, No. 4 ( 2006-04-01), p. 397-402
Abstract:
In the silkworm Bombyx mori, non-susceptibility to the Zhenjiang (China) strain of the densonucleosis virus (DNV-Z) is controlled by the recessive gene nsd-Z (non-susceptible to DNV-Z), which is located on chromosome 15. Owing to a lack of crossing over in females, reciprocal backcrossed F 1 (BC 1 ) progeny were used for linkage analysis and mapping of the nsd-Z gene using silkworm strains Js and L10, which are classified as being highly susceptible and non-susceptible to DNV-Z, respectively. BC 1 larvae were inoculated with the DNV-Z virus at the first instar, and DNA was extracted from the individual surviving pupae and analyzed for simple sequence repeat (SSR) markers. The nsd-Z gene was found to be linked to 7 SSR markers, as all the surviving larvae in the BC 1 ♀ (F 1 ♀ × L10♂) showed the homozygous profile of strain L10, and the sick larvae in the BC 1 ♀ (F 1 ♀ × L10♂) showed the heterozygous profile of Js × L10 F 1 hybrids. Using a reciprocal BC 1 ♂ (L10 1 ♀ × F 1 ♂) cross, we constructed a linkage map of 80.6 cM, with nsd-Z mapped at 30 cM and the closest SSR marker at a distance of 4.4 cM.Key words: SSR markers, densonucleosis virus, non-susceptible gene, silkworm, Bombyx mori.
Type of Medium:
Online Resource
ISSN:
0831-2796
,
1480-3321
Language:
English
Publisher:
Canadian Science Publishing
Publication Date:
2006
detail.hit.zdb_id:
2020635-5
SSG:
12
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