In:
PLOS Biology, Public Library of Science (PLoS), Vol. 22, No. 2 ( 2024-2-16), p. e3002505-
Abstract:
Alternative splicing is an essential regulatory mechanism for development and pathogenesis. Through alternative splicing one gene can encode multiple isoforms and be translated into proteins with different functions. Therefore, this diversity is an important dimension to understand the molecular mechanism governing embryo development. Isoform expression in preimplantation embryos has been extensively investigated, leading to the discovery of new isoforms. However, the dynamics of isoform switching of different types of transcripts throughout the development remains unexplored. Here, using single-cell direct isoform sequencing in over 100 single blastomeres from the mouse oocyte to blastocyst stage, we quantified isoform expression and found that 3-prime partial transcripts lacking stop codons are highly accumulated in oocytes and zygotes. These transcripts are not transcription by-products and might play a role in maternal to zygote transition (MZT) process. Long-read sequencing also enabled us to determine the expression of transposable elements (TEs) at specific loci. In this way, we identified 3,894 TE loci that exhibited dynamic changes along the preimplantation development, likely regulating the expression of adjacent genes. Our work provides novel insights into the transcriptional regulation of early embryo development.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3002505
DOI:
10.1371/journal.pbio.3002505.g001
DOI:
10.1371/journal.pbio.3002505.g002
DOI:
10.1371/journal.pbio.3002505.g003
DOI:
10.1371/journal.pbio.3002505.g004
DOI:
10.1371/journal.pbio.3002505.g005
DOI:
10.1371/journal.pbio.3002505.t001
DOI:
10.1371/journal.pbio.3002505.s001
DOI:
10.1371/journal.pbio.3002505.s002
DOI:
10.1371/journal.pbio.3002505.s003
DOI:
10.1371/journal.pbio.3002505.s004
DOI:
10.1371/journal.pbio.3002505.s005
DOI:
10.1371/journal.pbio.3002505.s006
DOI:
10.1371/journal.pbio.3002505.s007
DOI:
10.1371/journal.pbio.3002505.s008
DOI:
10.1371/journal.pbio.3002505.s009
DOI:
10.1371/journal.pbio.3002505.s010
DOI:
10.1371/journal.pbio.3002505.s011
DOI:
10.1371/journal.pbio.3002505.s012
DOI:
10.1371/journal.pbio.3002505.s013
DOI:
10.1371/journal.pbio.3002505.s014
DOI:
10.1371/journal.pbio.3002505.s015
DOI:
10.1371/journal.pbio.3002505.s016
DOI:
10.1371/journal.pbio.3002505.s017
DOI:
10.1371/journal.pbio.3002505.s018
DOI:
10.1371/journal.pbio.3002505.s019
DOI:
10.1371/journal.pbio.3002505.s020
DOI:
10.1371/journal.pbio.3002505.r001
DOI:
10.1371/journal.pbio.3002505.r002
DOI:
10.1371/journal.pbio.3002505.r003
DOI:
10.1371/journal.pbio.3002505.r004
DOI:
10.1371/journal.pbio.3002505.r005
DOI:
10.1371/journal.pbio.3002505.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2024
detail.hit.zdb_id:
2126773-X
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