In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 3 ( 2021-3-12), p. e1009388-
Abstract:
Phytophthora genomes encode a myriad of Crinkler (CRN) effectors, some of which contain putative kinase domains. Little is known about the host targets of these kinase-domain-containing CRNs and their infection-promoting mechanisms. Here, we report the host target and functional mechanism of a conserved kinase CRN effector named CRN78 in a notorious oomycete pathogen, Phytophthora sojae . CRN78 promotes Phytophthora capsici infection in Nicotiana benthamiana and enhances P . sojae virulence on the host plant Glycine max by inhibiting plant H 2 O 2 accumulation and immunity-related gene expression. Further investigation reveals that CRN78 interacts with PIP2-family aquaporin proteins including NbPIP2;2 from N . benthamiana and GmPIP2-13 from soybean on the plant plasma membrane, and membrane localization is necessary for virulence of CRN78. Next, CRN78 promotes phosphorylation of NbPIP2;2 or GmPIP2-13 using its kinase domain in vivo , leading to their subsequent protein degradation in a 26S-dependent pathway. Our data also demonstrates that NbPIP2;2 acts as a H 2 O 2 transporter to positively regulate plant immunity and reactive oxygen species (ROS) accumulation. Phylogenetic analysis suggests that the phosphorylation sites of PIP2 proteins and the kinase domains of CRN78 homologs are highly conserved among higher plants and oomycete pathogens, respectively. Therefore, this study elucidates a conserved and novel pathway used by effector proteins to inhibit host cellular defenses by targeting and hijacking phosphorylation of plant aquaporin proteins.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009388
DOI:
10.1371/journal.ppat.1009388.g001
DOI:
10.1371/journal.ppat.1009388.g002
DOI:
10.1371/journal.ppat.1009388.g003
DOI:
10.1371/journal.ppat.1009388.g004
DOI:
10.1371/journal.ppat.1009388.g005
DOI:
10.1371/journal.ppat.1009388.g006
DOI:
10.1371/journal.ppat.1009388.g007
DOI:
10.1371/journal.ppat.1009388.g008
DOI:
10.1371/journal.ppat.1009388.s001
DOI:
10.1371/journal.ppat.1009388.s002
DOI:
10.1371/journal.ppat.1009388.s003
DOI:
10.1371/journal.ppat.1009388.s004
DOI:
10.1371/journal.ppat.1009388.s005
DOI:
10.1371/journal.ppat.1009388.s006
DOI:
10.1371/journal.ppat.1009388.s007
DOI:
10.1371/journal.ppat.1009388.s008
DOI:
10.1371/journal.ppat.1009388.s009
DOI:
10.1371/journal.ppat.1009388.s010
DOI:
10.1371/journal.ppat.1009388.s011
DOI:
10.1371/journal.ppat.1009388.s012
DOI:
10.1371/journal.ppat.1009388.s013
DOI:
10.1371/journal.ppat.1009388.s014
DOI:
10.1371/journal.ppat.1009388.s015
DOI:
10.1371/journal.ppat.1009388.s016
DOI:
10.1371/journal.ppat.1009388.s017
DOI:
10.1371/journal.ppat.1009388.s018
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
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