In:
Intervirology, S. Karger AG, Vol. 54, No. 3 ( 2011), p. 113-121
Abstract:
〈 i 〉 Objective: 〈 /i 〉 ORF134 of 〈 i 〉 Bombyx mori 〈 /i 〉 nucleopolyhedrovirus (BmNPV) is a homolog of 〈 i 〉 Autographa californica 〈 /i 〉 multiple NPV ORF5, but its function is unknown so far. The aim of this study is to characterize BmNPV ORF134 (Bm134). 〈 i 〉 Methods: 〈 /i 〉 Transcription, protein expression and subcellular localization of Bm134 in BmN cells and silkworm midguts were analyzed using real-time polymerase chain reaction (RT-PCR), Western blot and immunofluorescence, respectively. 〈 i 〉 Results: 〈 /i 〉 Both the transcription and protein expression of Bm134 were not detected until 24 h post-infection (p.i.) by RT-PCR and Western blot, indicating that Bm134 is a late gene. Western blot revealed that Bm134 encodes an expected 12.4-kDa structural protein that is associated with occlusion-derived virus (ODV), not with budded virus. Immunofluorescence analysis showed that the Bm134 was first detected in the cytoplasm 24 h p.i. and then transported to the nucleus during later infection. Transcripts of Bm134 and the corresponding protein were only detected 48–72 h p.i. in BmNPV-infected larvae of 306, a highly BmNPV-susceptible silkworm strain, but not in the NB strain that is resistant to BmNPV infection. 〈 i 〉 Conclusion: 〈 /i 〉 Taken together, our data suggest that Bm134 is a late gene of BmNPV and may function as an ODV structural protein.
Type of Medium:
Online Resource
ISSN:
0300-5526
,
1423-0100
Language:
English
Publisher:
S. Karger AG
Publication Date:
2011
detail.hit.zdb_id:
1482863-7
SSG:
12
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