In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2018 ( 2018-11-14), p. 1-10
Abstract:
In this study, optimization of enzyme-assisted extraction, purification, characterization, and its bioactivities of polysaccharides from Hedyotis corymbosa (HCP) was investigated. It was found that the optimum extraction conditions were 3% of enzyme concentration ( X 1 ), 30 of liquid-to-solid ratio ( X 2 ), 56°C of extraction temperature ( X 3 ), 200W of ultrasonic power ( X 4 ), 10 min of extraction time (X 5 ), and 5 of pH value ( X 6 ). Under optimum conditions, the experimental yield (4.10 ± 0.16%) was closed to the predicted value (4.02%). The crude HCP was further purified using DEAE-52 and Sephadex G-150 gel column, and a major polysaccharide fraction from HCP, designed as HCP-1a with molecular weight of 33.9 kDa, was obtained. The HCP and HCP-1a were characterized by chemical analysis, FT-IR, and HPLC. For antioxidant activities in vitro , HCP possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe 2+ chelating activities. In subsequent immunostimulatory studies, significantly decreased NO, IL-1 β , and TNF- α concentrations were observed in both of HCP and HCP-1a treated RAW264.7 cells. Therefore, this study may indicate some insights into the application of polysaccharides from Hedyotis corymbosa as potential natural antioxidants and immunostimulants.
Type of Medium:
Online Resource
ISSN:
1741-427X
,
1741-4288
DOI:
10.1155/2018/8617070
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2018
detail.hit.zdb_id:
2148302-4
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