In:
Rapid Communications in Mass Spectrometry, Wiley, Vol. 19, No. 6 ( 2005-03-30), p. 775-780
Abstract:
A method using an online solid‐phase extraction (SPE) and ion‐pairing liquid chromatography with electrospray tandem mass spectrometry (LC/ES‐MS/MS) was developed for determination of amphetamine (Amp), methamphetamine (mAmp), 3,4‐methylenedioxyamphetamine (MDA), 3,4‐methylenedioxyethylamphetamine (MDEA), and 3,4‐methylenedioxymethamphetamine (MDMA) in urine samples. A SPE cartridge column with both hydrophilic and lipophilic functions was utilized for online extraction. A reversed‐phase C 18 LC column was employed for LC separation and MS/MS was used for detection. Trifluoroacetic acid was added to the mobile phase as an ion‐pairing reagent. This method was fully automated and the extraction and analysis procedures were controlled by a six‐port switch valve. Recoveries ranging from 85–101% were measured. Good linear ranges (10–500 ng/mL) for Amp and mAmp were determined. For MDA, MDMA and MDEA, dual linear ranges were obtained from 5–100 and 100–500 ng/mL, respectively. The detection limit of each analytical compound, based on a signal‐to‐noise ratio of 3, ranged from 1–3 ng/mL. The applicability of this newly developed method was examined by analyzing several urine samples from drug users. Good agreement was obtained between the results from this method and a literature GC/MS method. Copyright © 2005 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
0951-4198
,
1097-0231
Language:
English
Publisher:
Wiley
Publication Date:
2005
detail.hit.zdb_id:
2002158-6
detail.hit.zdb_id:
58731-X
SSG:
11
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