In:
Transfusion, Wiley, Vol. 55, No. 3 ( 2015-03), p. 586-592
Abstract:
Brucellosis is a severe zoonotic disease that is increasingly prevalent in north C hina. A study evaluating B rucella infection in blood donors was conducted at K ashi central blood station, X injiang, C hina. Study Design and Methods Four serologic and two molecular methods of detection of B rucella infection were used in plasma samples from blood donations collected from K ashi in northwest C hina, considered a brucellosis‐endemic area. Blood donor samples collected in S henzhen, southern C hina, a brucellosis‐nonendemic area, were tested as a negative control group. Results In 3896 plasma samples collected from K ashi central blood station, 135 (3.5%) plasma samples were reactive by the R ose B engal plate test ( RBPT ), and 120 (3.1%) of the 135 RBPT ‐reactive sample were also reactive with the standard tube agglutination test ( SAT ), respectively. All samples of the control group of 399 blood samples from S henzhen blood center tested negative with RBPT and SAT . Of 135 seroreactive plasma samples, 39 (1.0%) reacted with B . melitensis membrane protein extracts by enzyme‐linked immunosorbent assay and 25 were reactive to either r BP 26 or r OMP 31 by W estern blot. Thirteen plasma samples and two follow‐up blood samples were identified as carrying B rucella DNA by quantitative polymerase chain reaction ( PCR ) and nested PCR . Overall 15 (1:300) K ashi blood donations were found positive by nucleic acid testing, confirmed specific by DNA sequencing. Conclusions The data indicate a probable high rate of B rucella bacteremia, suggesting a potential risk of transfusion‐transmitted brucellosis. Blood donation screening for B rucella infection may be considered in the high B rucella ‐endemic areas of C hina.
Type of Medium:
Online Resource
ISSN:
0041-1132
,
1537-2995
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2018415-3
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