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  • 1
    Online Resource
    Online Resource
    A grooved conduit combined with decellularized tissues for peripheral nerve regeneration
    Springer Science and Business Media LLC ; 2023
    In:  Journal of Materials Science: Materials in Medicine Vol. 34, No. 7 ( 2023-07-21)
    Details
    In: Journal of Materials Science: Materials in Medicine, Springer Science and Business Media LLC, Vol. 34, No. 7 ( 2023-07-21)
    Abstract: Peripheral nerve injury (PNI) is a common and severe clinical disease worldwide, which leads to a poor prognosis because of the complicated treatments and high morbidity. Autologous nerve grafting as the gold standard still cannot meet the needs of clinical nerve transplantation because of its low availability and limited size. The development of artificial nerve conduits was led to a novel direction for PNI treatment, while most of the currently developed artificial nerve conduits was lack biochemical cues to promote nerve regeneration. In this study, we designed a novel composite neural conduit by inserting decellularized the rat sciatic nerve or kidney in a poly (lactic-co-glycolic acid) (PLGA) grooved conduit. The nerve regeneration effect of all samples was analyzed using rat sciatic nerve defect model, where decellularized tissues and grooved PLGA conduit alone were used as controls. The degree of nerve regeneration was evaluated using the motor function, gastrocnemius recovery, and morphological and histological assessments suggested that the combination of a grooved conduit with decellularized tissues significantly promoted nerve regeneration compared with decellularized tissues and PLGA conduit alone. It is worth to note that the grooved conduits containing decellularized nerves have a promotive effect similar to that of autologous nerve grafting, suggesting that it could be an artificial nerve conduit used for clinical practice in the future. Graphical Abstract
    Type of Medium: Online Resource
    ISSN: 1573-4838
    URL: Article
    DOI: 10.1007/s10856-023-06737-z
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2016995-4
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  • 2
    Online Resource
    Online Resource
    Decellularized porcine kidney-incorporated hydrogels for cell-laden bioprinting of renal cell carcinoma model
    AccScience Publishing ; 2024
    In:  International Journal of Bioprinting Vol. 10, No. 1 ( 2024-01-12), p. 1413-
    Details
    In: International Journal of Bioprinting, AccScience Publishing, Vol. 10, No. 1 ( 2024-01-12), p. 1413-
    Abstract: More than 90% of kidney cancers are attributed to renal cell carcinoma (RCC), which is however highly resistant to traditional chemotherapy. The challenges met in treating RCC signify an imperative to accelerate the development of new and effective drugs. Preclinical testing has served as a foundation for evaluating potential effectiveness of new drugs, but this endeavor is deeply restricted by the current generation of in vitro two-dimensional culture models, which cannot accurately mimic the tumor microenvironment (TME). Therefore, new in vitro three-dimensional (3D) cell culture models that can better mimic the components and architecture of TME have been developed for preclinical testing, but only a few existing 3D cell culture models can simulate the TME of RCC, representing a limitative obstacle impeding the development of novel drugs for RCC. In this study, we prepared a bioink by mixing porcine kidney decellularized extracellular matrix (dECM) powders with gelatin methacryloyl (GelMA) to bioprint an in vitro 3D cell culture model for RCC. We found that GelMA stability, mechanical properties, and printability were all significantly improved following the addition of the dECM powder. Moreover, cell cultures using ACHN cells suggested that kidney dECM powders significantly improved the cellular proliferation and metastasis via upregulation of markers related to epithelial & ndash; mesenchymal transition, along with activation of several cancer progression-related signaling pathways. More importantly, ACHN cells also demonstrated higher resistance to sunitinib under the stimulation of kidney dECM, indicating that GelMA-kidney dECM hydrogels may be an appropriate preclinical model to be used for building an in vitro RCC platform for drug screening and development.
    Type of Medium: Online Resource
    ISSN: 2424-7723 , 2424-8002
    URL: Article
    DOI: 10.36922/ijb.1413
    Language: Unknown
    Publisher: AccScience Publishing
    Publication Date: 2024
    detail.hit.zdb_id: 2834694-4
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  • 3
    Online Resource
    Online Resource
    In vitro study of decellularized rat tissues for nerve regeneration
    Frontiers Media SA ; 2022
    In:  Frontiers in Neurology Vol. 13 ( 2022-9-15)
    Details
    In: Frontiers in Neurology, Frontiers Media SA, Vol. 13 ( 2022-9-15)
    Abstract: Peripheral nerve injuries cause an absence or destruction of nerves. Decellularized nerves, acting as a replacement for autografts, have been investigated in the promotion of nerve repair and regeneration, always being incorporated with stem cells or growth factors. However, such a strategy is limited by size availability. The potential application in heterotopic transplantation of other decellularized tissues needs to be further explored. In this study, rat decellularized kidney (dK) was selected to be compared with decellularized peripheral nerve (dN), since dK has aboundant ECM components and growth factors. The PC-12 cells were cultured on dK and dN scaffolds, as shown in the similar behaviors of cell metabolism and viability, but have a more regular arrangement on dN compared to dK, indicating that the natural structure plays an important role in guiding cell extension. However, we found significant upregulation of axon–growth–associated genes and proteins of PC-12 cells in the dK group compared to the dN group by qRT-PCR, immunofluorescence, and western blotting. Furthermore, various neurotrophic factors and growth factors of acellular kidney and nerve were evaluated by ELISA assay. The lower expression of neurotrophic factors but higher expression of growth factors such as VEGF and HGF from dK suggests that axon growth and extension for PC-12 cells may be partially mediated by VEGF and HGF expression from decellularized kidney, which further points to a potential application in nerve repair and regeneration.
    Type of Medium: Online Resource
    ISSN: 1664-2295
    URL: Article
    DOI: 10.3389/fneur.2022.986377
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2564214-5
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  • 4
    Online Resource
    Online Resource
    3D bioprinting of multi-cellular tumor microenvironment for prostate cancer metastasis
    IOP Publishing ; 2023
    In:  Biofabrication Vol. 15, No. 3 ( 2023-07-01), p. 035020-
    Details
    In: Biofabrication, IOP Publishing, Vol. 15, No. 3 ( 2023-07-01), p. 035020-
    Abstract: Prostate cancer (PCa) is one of the most lethal cancers in men worldwide. The tumor microenvironment (TME) plays an important role in PCa development, which consists of tumor cells, fibroblasts, endothelial cells, and extracellular matrix (ECM). Hyaluronic acid (HA) and cancer-associated fibroblasts (CAFs) are the major components in the TME and are correlated with PCa proliferation and metastasis, while the underlying mechanism is still not fully understood due to the lack of biomimetic ECM components and coculture models. In this study, gelatin methacryloyl/chondroitin sulfate-based hydrogels were physically crosslinked with HA to develop a novel bioink for the three-dimensional bioprinting of a coculture model that can be used to investigate the effect of HA on PCa behaviors and the mechanism underlying PCa-fibroblasts interaction. PCa cells demonstrated distinct transcriptional profiles under HA stimulation, where cytokine secretion, angiogenesis, and epithelial to mesenchymal transition were significantly upregulated. Further coculture of PCa with normal fibroblasts activated CAF transformation, which could be induced by the upregulated cytokine secretion of PCa cells. These results suggested HA could not only promote PCa metastasis individually but also induce PCa cells to activate CAF transformation and form HA-CAF coupling effects to further promote PCa drug resistance and metastasis.
    Type of Medium: Online Resource
    ISSN: 1758-5082 , 1758-5090
    URL: Article
    DOI: 10.1088/1758-5090/acd960
    Language: Unknown
    Publisher: IOP Publishing
    Publication Date: 2023
    detail.hit.zdb_id: 2500944-8
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  • 5
    Online Resource
    Online Resource
    Evaluation of the Effect of Fibroblasts on Melanoma Metastasis Using a Biomimetic Co-Culture Model
    American Chemical Society (ACS) ; 2023
    In:  ACS Biomaterials Science & Engineering Vol. 9, No. 5 ( 2023-05-08), p. 2347-2361
    Details
    In: ACS Biomaterials Science & Engineering, American Chemical Society (ACS), Vol. 9, No. 5 ( 2023-05-08), p. 2347-2361
    Type of Medium: Online Resource
    ISSN: 2373-9878 , 2373-9878
    URL: Article
    URL: Article
    DOI: 10.1021/acsbiomaterials.2c01186
    DOI: 10.1021/acsbiomaterials.2c01186.s001
    Language: English
    Publisher: American Chemical Society (ACS)
    Publication Date: 2023
    detail.hit.zdb_id: 2806065-9
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  • 6
    Online Resource
    Online Resource
    Cells, growth factors and biomaterials used in tissue engineering for hair follicles regeneration
    Elsevier BV ; 2022
    In:  Regenerative Therapy Vol. 21 ( 2022-12), p. 596-610
    Details
    In: Regenerative Therapy, Elsevier BV, Vol. 21 ( 2022-12), p. 596-610
    Type of Medium: Online Resource
    ISSN: 2352-3204
    URL: Article
    DOI: 10.1016/j.reth.2022.11.003
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2022
    detail.hit.zdb_id: 2835333-X
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