In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 62, No. 1 ( 2012-01-01), p. 66-70
Abstract:
A mesophilic, aerotolerant, endospore-forming, fermentative bacterium, designated strain B1 T , was isolated from soil polluted by crude oil in the Karamay Oil Field, China. Cells were Gram-positive, rod-shaped, 1.1–1.6 µm wide and 2.3–4.7 µm long, and were motile by means of peritrichous flagella. Growth occurred at 10–40 °C and pH 6.0–8.9; optimal growth occurred at 28–32 °C and pH 7.3. The optimal concentrations of NaCl and sea salts for growth were 0.5 and 1 % (w/v), respectively. The strain was halotolerant and grew in the presence of NaCl or sea salts up to a concentration of 9 % (w/v). Substrates utilized as sole carbon sources were beef extract, yeast extract, peptone, tryptone, casein, d -glucose, d -fructose, d -xylose, d -ribose, d -galactose, maltose, l -rhamnose, trehalose, l -valine, dl -alanine plus l -proline and dl -alanine plus l -glycine. The main products of glucose fermentation were ethanol and acetate. iso-C 15 : 0 , iso-C 14 : 0 , C 16 : 0 and iso-C 13 : 0 were the major fatty acids. 16S rRNA gene sequence analysis revealed that the isolate belongs to the genus Tepidibacter , showing 94.7 and 94.1 % similarity to the type strains of Tepidibacter formicigenes and Tepidibacter thalassicus , respectively. The genomic DNA G+C content of strain B1 T was 29.8 mol%. On the basis of its phenotypic and genotypic properties, strain B1 T is suggested to represent a novel species of the genus Tepidibacter , for which the name Tepidibacter mesophilus sp. nov. is proposed. The type strain is B1 T ( = CGMCC 1.5148 T = JCM 16806 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.027409-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2012
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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