In:
Biotechnology and Applied Biochemistry, Wiley, Vol. 61, No. 6 ( 2014-11), p. 637-645
Abstract:
L ycium barbarum contains high levels of zeaxanthin, which is produced by the conversion of ‐carotene into zeaxanthin. ‐Carotene hydroxylase catalyzes this reaction. We cloned a c DNA ( chyb ) encoding ‐carotene hydroxylase ( C hyb) from the L . barbarum leaf. A 939‐bp full‐length c DNA sequence was determined with 3′‐rapid amplification of c DNA end assay encoding a deduced C hyb protein (34.8 kDa) with a theoretical isoelectric point of 8.36. A bioinformatics analysis showed that the L . barbarum C hyb was located in the chloroplast. Further, to investigate the catalytic activity of the L . barbarum C hyb, a complementation analysis was conducted in E scherichia coli . The results strongly demonstrated that C hyb can catalyze ‐carotene to produce zeaxanthin. Thus, this study suggests that L . barbarum ‐carotene hydroxylase could be a means of zeaxanthin production by genetic manipulation in E . coli .
Type of Medium:
Online Resource
ISSN:
0885-4513
,
1470-8744
DOI:
10.1002/bab.2014.61.issue-6
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
1496341-3
SSG:
12
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