GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Magnetotactic Bacteria and Magnetosomes as Smart Drug Delivery Systems: A New Weapon on the Battlefield with Cancer?
    MDPI AG ; 2020
    In:  Biology Vol. 9, No. 5 ( 2020-05-19), p. 102-
    Details
    In: Biology, MDPI AG, Vol. 9, No. 5 ( 2020-05-19), p. 102-
    Abstract: An important direction of research in increasing the effectiveness of cancer therapies is the design of effective drug distribution systems in the body. The development of the new strategies is primarily aimed at improving the stability of the drug after administration and increasing the precision of drug delivery to the destination. Due to the characteristic features of cancer cells, distributing chemotherapeutics exactly to the microenvironment of the tumor while sparing the healthy tissues is an important issue here. One of the promising solutions that would meet the above requirements is the use of Magnetotactic bacteria (MTBs) and their organelles, called magnetosomes (BMs). MTBs are commonly found in water reservoirs, and BMs that contain ferromagnetic crystals condition the magnetotaxis of these microorganisms. The presented work is a review of the current state of knowledge on the potential use of MTBs and BMs as nanocarriers in the therapy of cancer. The growing amount of literature data indicates that MTBs and BMs may be used as natural nanocarriers for chemotherapeutics, such as classic anti-cancer drugs, antibodies, vaccine DNA, and siRNA. Their use as transporters increases the stability of chemotherapeutics and allows the transfer of individual ligands or their combinations precisely to cancerous tumors, which, in turn, enables the drugs to reach molecular targets more effectively.
    Type of Medium: Online Resource
    ISSN: 2079-7737
    URL: Article
    DOI: 10.3390/biology9050102
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2661517-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Neonatal breast-suckling skills in the context of lactation and peripartum hormonal changes and additional factors—a pilot study
    Springer Science and Business Media LLC ; 2022
    In:  International Breastfeeding Journal Vol. 17, No. 1 ( 2022-09-01)
    Details
    In: International Breastfeeding Journal, Springer Science and Business Media LLC, Vol. 17, No. 1 ( 2022-09-01)
    Abstract: Childbirth and lactation are intricate processes, involving several hormones, the most important of which are prolactin (a protein hormone) and cortisol (one of the glucocorticoids). The early postpartum period is crucial for both mother and newborn and has an impact on the lactation and breastfeeding process. Methods The study included 78 patients who were admitted to the Gynecology-Obstetrics Clinical Hospital in Poznań for labor induction and/or in the active phase of the first labor stage. The levels of cortisol and prolactin in serum were assessed in these women during admission in labor, during the third labor stage, and on the second day postpartum. The levels of cortisol and prolactin in the umbilical cord serum were assessed immediately after cord clamping. The “Protocol for the assessment of breast-suckling skills” was used to assess the neonatal breast-suckling skills on the second day postpartum. Some additional parameters were evaluated in mothers via a telephone interview at three and six months postpartum. The study was conducted from January to August 2020, however the study was suspended during April–July 2020 due to the SARS-CoV-2 pandemic, which led to restrictions in the hospital limiting access to the hospital wards unless necessary. Results Early breastfeeding with skin-to-skin contact was associated with low levels of hormones, cortisol levels were lower in serum ( p  = 0.0108) and umbilical vein ( p  = 0.0273) in mothers who breastfed immediately after childbirth. At three months postpartum, 88% of the mothers who did not offer a pacifier to the child during the first few days of life breastfed the child naturally ( p  = 0.037), and at six months, 96% of those who did not offer a pacifier continued to breastfeed ( p  = 0.0008). Multiple, statistically significant correlations were observed between the variables assessed according to the “Protocol for the assessment of breast-suckling skills” and breastfeeding after three months. Conclusions Breastfeeding immediately after childbirth, appropriate assessment of the breast-suckling skills of newborns, avoiding pacifiers and infant formula feeding, and offering support to new mothers in the early days after childbirth seem to be important factors for sustaining breastfeeding after three and six months of childbirth.
    Type of Medium: Online Resource
    ISSN: 1746-4358
    URL: Article
    DOI: 10.1186/s13006-022-00508-2
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2227239-2
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Recombinant AroL‐Catalyzed Phosphorylation for the Efficient Synthesis of Shikimic Acid 3‐Phosphate
    Wiley ; 2018
    In:  Biotechnology Journal Vol. 13, No. 8 ( 2018-08)
    Details
    In: Biotechnology Journal, Wiley, Vol. 13, No. 8 ( 2018-08)
    Abstract: Shikimic acid 3‐phosphate, as a central metabolite of the shikimate pathway, is of high interest as enzyme substrate for 5‐enolpyruvoyl‐shikimate 3‐phosphate synthase, a drug target in infectious diseases and a prime enzyme target for the herbicide glyphosate. As the important substrate shikimic acid 3‐phosphate is only accessible via a chemical multi‐step route, a new straightforward preparative one‐step enzymatic phosphorylation of shikimate using a stable recombinant shikimate kinase has been developed for the selective phosphorylation of shikimate in the 3‐position. Highly active shikimate kinase is produced by straightforward expression of a synthetic aroL gene in Escherichia coli . The time course of the shikimate kinase‐catalyzed phosphorylation is investigated by 1 H‐ and 31 P‐NMR, using the phosphoenolpyruvate/pyruvate kinase system for the regeneration of the ATP cofactor. This enables the development of a quantitative biocatalytic 3‐phosphorylation of shikimic acid. After a standard workup procedure, a good yield of shikimic acid 3‐phosphate, with high HPLC‐ and NMR purity, is obtained. This efficient biocatalytic synthesis of shikimic acid 3‐phosphate is superior to any other method and has been successfully scaled up to multi‐gram scale.
    Type of Medium: Online Resource
    ISSN: 1860-6768 , 1860-7314
    URL: Issue
    URL: Article
    DOI: 10.1002/biot.v13.8
    DOI: 10.1002/biot.201700529
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2214038-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Synthesis of N ω ‐Phospho‐ l ‐arginine by Biocatalytic Phosphorylation of l ‐Arginine
    Wiley ; 2017
    In:  ChemCatChem Vol. 9, No. 1 ( 2017-01-09), p. 121-126
    Details
    In: ChemCatChem, Wiley, Vol. 9, No. 1 ( 2017-01-09), p. 121-126
    Abstract: The N ω ‐phospho‐ l ‐arginine energy‐buffering system is mainly present in invertebrates for regulating energy requirements when it is highly needed, such as in the flight muscles of an insect or when energy supply fluctuates, as in the medically important protozoa Trypanosoma brucei , Trypanosoma cruzi , and Leishmania major . The lack of availability of this important metabolite was due to a tedious chemical procedure, by which N ω ‐phospho‐ l ‐arginine was prepared in over 5 reaction steps in a low yield. Therefore, we aimed at improving the synthetic methodology for the preparation of this important metabolite. As site‐ and enantioselective kinases have been very useful catalysts for biocatalytic phosphorylations in straightforward syntheses of phosphorylated metabolites, a stable and selective arginine kinase has been selected for the selective phosphorylation of l ‐arginine. The Arg gene has been cloned and expressed in E. coli and a highly active arginine kinase has been prepared. A simple synthesis of N ω ‐phospho‐ l ‐arginine has been developed by arginine kinase catalyzed phosphorylation of l ‐arginine combined with the recycling of the phosphorylating agent ATP by using the phosphoenolpyruvate/pyruvate kinase system. After standard work‐up, the desired product N ω ‐phospho‐ l ‐arginine was obtained in gram quantities and in one step.
    Type of Medium: Online Resource
    ISSN: 1867-3880 , 1867-3899
    URL: Issue
    URL: Article
    DOI: 10.1002/cctc.v9.1
    DOI: 10.1002/cctc.201601080
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2501161-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Monooksygenazy cytochromu P450 – wszechstronne biokatalizatory
    Polskie Towarzystwo Biochemiczne (Polish Biochemical Society) ; 2022
    In:  Postępy Biochemii ( 2022-11-12)
    Details
    In: Postępy Biochemii, Polskie Towarzystwo Biochemiczne (Polish Biochemical Society), ( 2022-11-12)
    Abstract: Postępy w biochemii pomogły zrozumieć strukturę i funkcję enzymów, co z kolei doprowadziło do zwiększenia ich stabilności, aktywności i specyficzności substratowej. Obecnie biokataliza zapewnia bardziej zrównoważone, wydajne i mniej zanieczyszczające metody produkcji wysokowartościowych chemikaliów i zaawansowanych półproduktów farmaceutycznych. W niniejszej pracy przedstawiono strukturę oraz mechanizm działania monooksygenaz cytochromu P450 oraz ich zastosowanie w efektywnej syntezy związków biologicznie czynnych, która jest bardziej ekologiczna, mniej czasochłonna i tańsza w porównaniu z syntezą chemiczną. Przemysł farmaceutyczny powinien wykorzystać zalety postępu biochemii do pozyskiwania biokatalizatorów do produkcji wysokowartościowych chemikaliów na skalę przemysłową, poprawiając jakość produktów końcowych jednocześnie zmniejszając koszty.
    Type of Medium: Online Resource
    ISSN: 0032-5422
    URL: Article
    DOI: 10.18388/pb.2021_464
    Language: Unknown
    Publisher: Polskie Towarzystwo Biochemiczne (Polish Biochemical Society)
    Publication Date: 2022
    detail.hit.zdb_id: 3141308-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Modified Baby Milk—Bioelements Composition and Toxic Elements Contamination
    MDPI AG ; 2021
    In:  Molecules Vol. 26, No. 14 ( 2021-07-09), p. 4184-
    Details
    In: Molecules, MDPI AG, Vol. 26, No. 14 ( 2021-07-09), p. 4184-
    Abstract: Breast milk has the most suitable composition for the proper development in the first year of a child’s life. However, it is often replaced with artificial milk. The aim of the study was to analyze the composition of essential elements: Na, K, Ca, P, Mg, Fe, Zn, Cu, and Mn as well as toxic elements: Ni, Pb, Sr, Li, and In in 18 formulas available in Poland. The daily supply was also estimated. The study was performed by Inductively Coupled Plasma Optical Emission Spectrometry method. The results showed the presence of all essential elements tested, but the content of P and Mn significantly differed from the concentrations declared. Such discrepancies can have significant impact on the daily dose of the bioelements taken. However, the content of elements was within the reference standards established by the EU Directive with exception of P, the amount of which exceeded the norms 5.23–18.80-times. Daily supply of P in tested milk as well as Fe and Mn provided with first and hypoallergenic formula exceeded the adequate intake. Analysis revealed the contamination with harmful elements—Pb, Sr, Li, and In were detected in almost all products. The study confirms the data concerning some discrepancies in composition and the contamination of food and may provide information on the feeding quality of children and estimation of health risk associated with exposure to toxic elements.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    URL: Article
    DOI: 10.3390/molecules26144184
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2008644-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Left atrial accessory appendages, diverticula, and left-sided septal pouch in multi-slice computed tomography. Association with atrial fibrillation and cerebrovascular accidents
    Elsevier BV ; 2017
    In:  International Journal of Cardiology Vol. 244 ( 2017-10), p. 163-168
    Details
    In: International Journal of Cardiology, Elsevier BV, Vol. 244 ( 2017-10), p. 163-168
    Type of Medium: Online Resource
    ISSN: 0167-5273
    URL: Article
    DOI: 10.1016/j.ijcard.2017.06.042
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2017
    detail.hit.zdb_id: 1500478-8
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Blood-Stained Colostrum: A Rare Phenomenon at an Early Lactation Stage
    MDPI AG ; 2022
    In:  Children Vol. 9, No. 2 ( 2022-02-06), p. 213-
    Details
    In: Children, MDPI AG, Vol. 9, No. 2 ( 2022-02-06), p. 213-
    Abstract: The phenomenon described in the literature as rusty pipe syndrome is a rare condition (the estimated incidence is 0.1% in the population of breastfeeding women) where the prenatal milk and the colostrum are rust- or blood-colored. Due to the rare occurrence of this phenomenon and the related nature of the baby’s regurgitated discharge—green, brown or blood-stained, there is a general fear of latching a newborn or continuing to breastfeed if the regurgitation persists. In this care report, a patient’s milk was tested to determine its microbiological and morphological content. No significant abnormalities were noted in these tests. The nutritional profile of the blood-stained colostrum was normal. Breast milk has an indisputably invaluable impact on the newborn’s further development and there is no connection between rusty pipe syndrome, as described in the literature, and any clinical complications. This is crucial to encourage mothers to keep breastfeeding even if they observe blood-stained colostrum.
    Type of Medium: Online Resource
    ISSN: 2227-9067
    URL: Article
    DOI: 10.3390/children9020213
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2732685-8
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Peripartum prolactin and cortisol level changes. A prospective pilot study
    VM Media Group sp. z o.o ; 2022
    In:  Ginekologia Polska
    Details
    In: Ginekologia Polska, VM Media Group sp. z o.o
    Type of Medium: Online Resource
    ISSN: 2543-6767 , 0017-0011
    URL: Article
    DOI: 10.5603/GP.a2022.0125
    Language: Unknown
    Publisher: VM Media Group sp. z o.o
    Publication Date: 2022
    detail.hit.zdb_id: 2601802-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Highly selective bile acid hydroxylation by the multifunctional bacterial P450 monooxygenase CYP107D1 (OleP)
    Springer Science and Business Media LLC ; 2020
    In:  Biotechnology Letters Vol. 42, No. 5 ( 2020-05), p. 819-824
    Details
    In: Biotechnology Letters, Springer Science and Business Media LLC, Vol. 42, No. 5 ( 2020-05), p. 819-824
    Abstract: Regio- and stereoselective hydroxylation of lithocholic acid (LCA) using CYP107D1 (OleP), a cytochrome P450 monooxygenase from the oleandomycin synthesis pathway of Streptomyces antibioticus . Results Co-expression of CYP107D1 from S. antibioticus and the reductase/ferredoxin system PdR/PdX from Pseudomonas putida was performed in Escherichia coli whole cells. In vivo hydroxylation of LCA exclusively yielded the 6β-OH product murideoxycholic acid (MDCA). In resting cells, 19.5% of LCA was converted to MDCA within 24 h, resulting in a space time yield of 0.04 mmol L −1 h −1 . NMR spectroscopy confirmed the identity of MDCA as the sole product. Conclusions The multifunctional P450 monooxygenase CYP107D1 (OleP) can hydroxylate LCA, forming MDCA as the only product.
    Type of Medium: Online Resource
    ISSN: 0141-5492 , 1573-6776
    URL: Article
    DOI: 10.1007/s10529-020-02813-4
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2012203-2
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...