In:
The Journal of Immunology, The American Association of Immunologists, Vol. 178, No. 1_Supplement ( 2007-04-01), p. S16-S17
Abstract:
Activating DCs produce reactive oxygen species (ROS), which are necessary during antigen processing. In this study, we hypothesized that vitamin C (VC), as an antioxidant, may affect phenotype and function of DCs following activation. Bone marrow cells isolated from mice were cultured and differentiated into DCs in the presence of GM-CSF for 7 days. DCs were pre-treated with various concentrations of VC, and activated with LPS for 24 hrs. The supernatants were collected for measurements of cytokine secretion, and the changes of several markers of DC activation were analyzed. VC in the culture media reduced intracellular ROS levels, suggesting anti-oxidant effects on DCs. The expression of CD40 and CD80 were not influenced, but those of CD86 and MHC class II were slightly reduced. The reduction of phagocytic functions of DCs with activation was suppressed by the presence of VC, dose-dependently. In the supernatants, dose-dependent reduction of IL-6 and increased secretion of IL-12p70 and IL-10 were observed with increasing concentration of VC. IFN-¥ã was not detected at all. Because VC affected some of surface marker expression and phagocytic functions of activated DCs and, most noticeably, changed cytokine secretion profiles, we also investigated the results of T cell activation by those DCs treated with VC, with respect to T cell proliferation and cytokine secretion by activated T cells.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.178.Supp.36.24
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2007
detail.hit.zdb_id:
1475085-5
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