In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 2 ( 2023-2-6), p. e0278325-
Abstract:
Microglia are the immune effector cells of the central nervous system (CNS) and react to pathologic events with a complex process including the release of nitric oxide (NO). NO is a free radical, which is toxic for all cells at high concentrations. To target an exaggerated NO release, we tested a library of 16 544 chemical compounds for their effect on lipopolysaccharide (LPS)-induced NO release in cell line and primary neonatal microglia. We identified a compound (C1) which significantly reduced NO release in a dose-dependent manner, with a low IC 50 (252 nM) and no toxic side effects in vitro or in vivo . Target finding strategies such as in silico modelling and mass spectroscopy hint towards a direct interaction between C1 and the nitric oxide synthase making C1 a great candidate for specific intra-cellular interaction with the NO producing machinery.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0278325
DOI:
10.1371/journal.pone.0278325.g001
DOI:
10.1371/journal.pone.0278325.g002
DOI:
10.1371/journal.pone.0278325.g003
DOI:
10.1371/journal.pone.0278325.g004
DOI:
10.1371/journal.pone.0278325.g005
DOI:
10.1371/journal.pone.0278325.g006
DOI:
10.1371/journal.pone.0278325.g007
DOI:
10.1371/journal.pone.0278325.g008
DOI:
10.1371/journal.pone.0278325.s001
DOI:
10.1371/journal.pone.0278325.s002
DOI:
10.1371/journal.pone.0278325.s003
DOI:
10.1371/journal.pone.0278325.s004
DOI:
10.1371/journal.pone.0278325.s005
DOI:
10.1371/journal.pone.0278325.s006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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