GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    The p50 Subunit of NF-κB Is Critical for In Vivo Clearance of the Noninvasive Enteric Pathogen Citrobacter rodentium
    American Society for Microbiology ; 2008
    In:  Infection and Immunity Vol. 76, No. 11 ( 2008-11), p. 4978-4988
    Details
    In: Infection and Immunity, American Society for Microbiology, Vol. 76, No. 11 ( 2008-11), p. 4978-4988
    Abstract: Citrobacter rodentium , a natural mouse pathogen, belongs to the family of extracellular enteric pathogens that includes enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC). C. rodentium shares many virulence factors with EPEC and EHEC and relies on attaching-and-effacing lesion formation for colonization and infection of the gut. In vivo, C. rodentium infection is characterized by increased epithelial cell proliferation, mucosal thickening, and a TH1-type immune response, but with protective immunity believed to be mediated by serum immunoglobulin G (IgG). In this work, we characterize the immune response and pathology of mice lacking the p50 subunit of the transcription factor nuclear factor kappa B (NF-κB) during C. rodentium infection. We show that p50 −/− mice are unable to clear C. rodentium infection. Furthermore, these animals show a reduced influx of immune cells into infected colonic tissue and greater levels of mucosal hyperplasia and the cytokines tumor necrosis factor alpha and gamma interferon. Surprisingly, despite being unable to eliminate infection, p50 −/− mice showed markedly higher levels of anti- Citrobacter IgG and IgM, suggesting that antibody alone is not responsible for bacterial clearance. These data also demonstrate that non-NF-κB-dependent defenses are insufficient to control C. rodentium infection, and hence, the NF-κB p50 subunit is critical for defense against this noninvasive pathogen.
    Type of Medium: Online Resource
    ISSN: 0019-9567 , 1098-5522
    URL: Article
    DOI: 10.1128/IAI.00736-08
    RVK:
    XA 10000
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2008
    detail.hit.zdb_id: 1483247-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Staphylococcal Protein A Contributes to Persistent Colonization of Mice with Staphylococcus aureus
    American Society for Microbiology ; 2018
    In:  Journal of Bacteriology Vol. 200, No. 9 ( 2018-05)
    Details
    In: Journal of Bacteriology, American Society for Microbiology, Vol. 200, No. 9 ( 2018-05)
    Abstract: Staphylococcus aureus persistently colonizes the nasopharynx in humans, which increases the risk for invasive diseases, such as skin infection and bacteremia. Nasal colonization triggers IgG responses against staphylococcal surface antigens; however, these antibodies cannot prevent subsequent colonization or disease. Here, we describe S. aureus WU1, a multilocus sequence type 88 (ST88) isolate that persistently colonizes the nasopharynx in mice. We report that staphylococcal protein A (SpA) is required for persistence of S. aureus WU1 in the nasopharynx. Compared to animals colonized by wild-type S. aureus , mice colonized with the Δ spa variant mount increased IgG responses against staphylococcal colonization determinants. Immunization of mice with a nontoxigenic SpA variant, which cannot cross-link B cell receptors and divert antibody responses, elicits protein A-neutralizing antibodies that promote IgG responses against colonizing S. aureus and diminish pathogen persistence. IMPORTANCE Staphylococcus aureus persistently colonizes the nasopharynx in about one-third of the human population, thereby promoting community- and hospital-acquired infections. Antibiotics are currently used for decolonization of individuals at increased risk of infection. However, the efficacy of antibiotics is limited by recolonization and selection for drug-resistant strains. Here, we propose a model of how staphylococcal protein A (SpA), a B cell superantigen, modifies host immune responses during colonization to support continued persistence of S. aureus in the nasopharynx. We show that this mechanism can be thwarted by vaccine-induced anti-SpA antibodies that promote IgG responses against staphylococcal antigens and diminish colonization.
    Type of Medium: Online Resource
    ISSN: 0021-9193 , 1098-5530
    URL: Article
    DOI: 10.1128/JB.00735-17
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2018
    detail.hit.zdb_id: 1481988-0
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Data S1: Raw bioluminescence data
    PeerJ ; 2016
    In:  PeerJ Vol. 4 ( 2016-11-22), p. e2717-
    Details
    In: PeerJ, PeerJ, Vol. 4 ( 2016-11-22), p. e2717-
    Abstract: Much is known regarding the antibiotic susceptibility of planktonic cultures of Mycobacterium tuberculosis , the bacterium responsible for the lung disease tuberculosis (TB). As planktonically-grown M. tuberculosis are unlikely to be entirely representative of the bacterium during infection, we set out to determine how effective a range of anti-mycobacterial treatments were against M. tuberculosis growing as a biofilm, a bacterial phenotype known to be more resistant to antibiotic treatment. Light levels from bioluminescently-labelled M. tuberculosis H37Rv (strain BSG001) were used as a surrogate for bacterial viability, and were monitored before and after one week of treatment. After treatment, biofilms were disrupted, washed and inoculated into fresh broth and plated onto solid media to rescue any surviving bacteria. We found that in this phenotypic state M. tuberculosis was resistant to the majority of the compounds tested. Minimum inhibitory concentrations (MICs) increased by 20-fold to greater than 1,000-fold, underlying the potential of this phenotype to cause significant problems during treatment.
    Type of Medium: Online Resource
    ISSN: 2167-8359
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.7717/peerj.2717
    DOI: 10.7717/peerj.2717/fig-1
    DOI: 10.7717/peerj.2717/fig-2
    DOI: 10.7717/peerj.2717/fig-3
    DOI: 10.7717/peerj.2717/table-1
    DOI: 10.7717/peerj.2717/supp-1
    Language: English
    Publisher: PeerJ
    Publication Date: 2016
    detail.hit.zdb_id: 2703241-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Data S1: Raw data of bioluminescence measurements from PANI and P3ABA treated mycobacterial cells used in the preparation of Figs. 1–3
    PeerJ ; 2016
    In:  PeerJ Vol. 4 ( 2016-12-20), p. e2795-
    Details
    In: PeerJ, PeerJ, Vol. 4 ( 2016-12-20), p. e2795-
    Abstract: Tuberculosis is considered a leading cause of death worldwide. More than 95% of cases and deaths occur in low- and middle-income countries. In resource-limited countries, hospitals often lack adequate facilities to manage and isolate patients with infectious tuberculosis (TB), relying instead on personal protective equipment, such as facemasks, to reduce nosocomial transmission of the disease. Facemasks impregnated with an antimicrobial agent may be a cost-effective way of adding an extra level of protection against the spread of TB by reducing the risk of disease transmission. Conducting polymers, such as polyaniline (PANI), and their functionalised derivatives are a novel class of antimicrobial agents with potential as non-leaching additives to provide contamination resistant surfaces. We have investigated the antimicrobial action of PANI and a functionalised derivative, poly-3-aminobenzoic acid (P3ABA), against mycobacteria and have determined the optimal treatment time and concentration to achieve significant knockdown of Mycobacterium smegmatis and Mycobacterium tuberculosis on an agar surface. Results indicated that P3ABA is a potential candidate for use as an anti-tuberculoid agent in facemasks to reduce TB transmission.
    Type of Medium: Online Resource
    ISSN: 2167-8359
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.7717/peerj.2795
    DOI: 10.7717/peerj.2795/fig-1
    DOI: 10.7717/peerj.2795/fig-2
    DOI: 10.7717/peerj.2795/fig-3
    DOI: 10.7717/peerj.2795/supp-1
    Language: English
    Publisher: PeerJ
    Publication Date: 2016
    detail.hit.zdb_id: 2703241-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Exploring the depth and breadth of the genomics toolbox during the COVID-19 pandemic: insights from Aotearoa New Zealand
    Springer Science and Business Media LLC ; 2023
    In:  BMC Medicine Vol. 21, No. 1 ( 2023-06-14)
    Details
    In: BMC Medicine, Springer Science and Business Media LLC, Vol. 21, No. 1 ( 2023-06-14)
    Abstract: Genomic technologies have become routine in the surveillance and monitoring of the coronavirus disease 2019 (COVID-19) pandemic, as evidenced by the millions of SARS-CoV-2 sequences uploaded to international databases. Yet the ways in which these technologies have been applied to manage the pandemic are varied. Main text Aotearoa New Zealand was one of a small number of countries to adopt an elimination strategy for COVID-19, establishing a managed isolation and quarantine system for all international arrivals. To aid our response, we rapidly set up and scaled our use of genomic technologies to help identify community cases of COVID-19, to understand how they had arisen, and to determine the appropriate action to maintain elimination. Once New Zealand pivoted from elimination to suppression in late 2021, our genomic response changed to focusing on identifying new variants arriving at the border, tracking their incidence around the country, and examining any links between specific variants and increased disease severity. Wastewater detection, quantitation and variant detection were also phased into the response. Here, we explore New Zealand’s genomic journey through the pandemic and provide a high-level overview of the lessons learned and potential future capabilities to better prepare for future pandemics. Conclusions Our commentary is aimed at health professionals and decision-makers who might not be familiar with genetic technologies, how they can be used, and why this is an area with great potential to assist in disease detection and tracking now and in the future.
    Type of Medium: Online Resource
    ISSN: 1741-7015
    URL: Article
    DOI: 10.1186/s12916-023-02909-4
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2023
    detail.hit.zdb_id: 2131669-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Ecological and physiological analyses of Pseudomonad species within a phenol remediation system
    Elsevier BV ; 2001
    In:  Journal of Microbiological Methods Vol. 44, No. 1 ( 2001-2), p. 79-88
    Details
    In: Journal of Microbiological Methods, Elsevier BV, Vol. 44, No. 1 ( 2001-2), p. 79-88
    Type of Medium: Online Resource
    ISSN: 0167-7012
    URL: Article
    DOI: 10.1016/S0167-7012(00)00231-1
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2001
    detail.hit.zdb_id: 1483012-7
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    A Helping Hand during t-Testing Times
    Public Library of Science (PLoS) ; 2014
    In:  PLoS Biology Vol. 12, No. 2 ( 2014-2-18), p. e1001785-
    Details
    In: PLoS Biology, Public Library of Science (PLoS), Vol. 12, No. 2 ( 2014-2-18), p. e1001785-
    Type of Medium: Online Resource
    ISSN: 1545-7885
    URL: Article
    URL: Article
    DOI: 10.1371/journal.pbio.1001785
    DOI: 10.1371/journal.pbio.1001785.g001
    Language: English
    Publisher: Public Library of Science (PLoS)
    Publication Date: 2014
    detail.hit.zdb_id: 2126773-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Use of biophotonic imaging as a training aid for administration of substances in laboratory rodents
    SAGE Publications ; 2007
    In:  Laboratory Animals Vol. 41, No. 3 ( 2007-07-01), p. 321-328
    Details
    In: Laboratory Animals, SAGE Publications, Vol. 41, No. 3 ( 2007-07-01), p. 321-328
    Abstract: Dosing of experimental animals and the removal of blood are two of the most frequent procedures performed in biomedical research using live animals. Despite the apparently simple nature of these procedures, they can, if not correctly carried out, have significant effects on the welfare of the animals and the scientific value of the results. There are several methods by which research staff may obtain training in the administration of substances. These include practical demonstrations during teaching courses; observation of techniques; videos and educational computer programs and practising on recently killed animal cadavers or plastic animal models. Each method has its own advantages and disadvantages. A common factor encountered during training is the difficulty in assessing competency. This paper reports a pilot study on the use of bioluminescent imaging technology to assess competency in the administration of substances to rodents. Bioluminescence was rapidly detected after dosing of animals with a bioluminescent substance. However, living animals were required for a signal to be generated. The data presented suggest that this technology is ideal for use as a teaching aid and may also prove valuable in assessing the effectiveness of 'complex' and novel administration routes in 'realtime'.
    Type of Medium: Online Resource
    ISSN: 0023-6772 , 1758-1117
    URL: Article
    DOI: 10.1258/002367707781282785
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2007
    detail.hit.zdb_id: 2036511-1
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Going viral: A science communication collaboration in the era of COVID-19 and social media
    Frontiers Media SA ; 2023
    In:  Frontiers in Communication Vol. 8 ( 2023-2-27)
    Details
    In: Frontiers in Communication, Frontiers Media SA, Vol. 8 ( 2023-2-27)
    Abstract: On 9 March 2020, 2 days before the World Health Organization declared COVID-19 a global pandemic, two of the authors (microbiologist and infectious diseases expert Associate Professor Siouxsie Wiles and cartoonist Toby Morris) released their first output together: an animated GIF (Graphics Interchange Format) known as “Flatten the Curve”. The graphic went viral on Twitter with over 10 million impressions in 3 days. Flatten the Curve was the first of more than 70 graphics produced by our collaboration, all designed as accessible visual communication about COVID-19. The graphics, all released under a Creative Commons CC-BY-SA-4.0 license, have been translated into multiple languages, used by communities, politicians, and public health officials around the world, and the collaborators have won multiple awards for their work.
    Type of Medium: Online Resource
    ISSN: 2297-900X
    URL: Article
    DOI: 10.3389/fcomm.2023.1087120
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2856337-2
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Noninvasive biophotonic imaging for studies of infectious disease
    Oxford University Press (OUP) ; 2011
    In:  FEMS Microbiology Reviews Vol. 35, No. 2 ( 2011-03-01), p. 360-394
    Details
    In: FEMS Microbiology Reviews, Oxford University Press (OUP), Vol. 35, No. 2 ( 2011-03-01), p. 360-394
    Type of Medium: Online Resource
    ISSN: 1574-6976 , 0168-6445
    URL: Article
    DOI: 10.1111/j.1574-6976.2010.00252.x
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2011
    detail.hit.zdb_id: 1500468-5
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...