In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 4 ( 2023-4-21), p. e0284135-
Abstract:
We have developed a cell-based outer vocal fold replacement (COVR) as a potential therapy to improve voice quality after vocal fold (VF) injury, radiation, or tumor resection. The COVR consists of multipotent human adipose-derived stem cells (hASC) embedded within a three-dimensional fibrin scaffold that resembles vocal fold epithelium and lamina propria layers. Previous work has shown improved wound healing in rabbit studies. In this pilot study in pigs, we sought to develop methods for large animal implantation and phonatory assessment. Feasibility, safety, and structural and functional outcomes of the COVR implant are described. Of eight pigs studied, six animals underwent COVR implantation with harvest between 2 weeks and 6 months. Recovery of laryngeal tissue structure was assessed by vibratory and histologic analyses. Recovery of voice function was assessed by investigating acoustic parameters that were derived specifically for pigs. Results showed improved lamina propria qualities relative to an injured control animal at 6 months. Acoustic parameters reflected voice worsening immediately after surgery as expected; acoustics displayed clear voice recovery in the animal followed for 6 months after COVR. These methods form the basis for a larger-scale long-term pre-clinical safety and efficacy study.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0284135
DOI:
10.1371/journal.pone.0284135.g001
DOI:
10.1371/journal.pone.0284135.g002
DOI:
10.1371/journal.pone.0284135.g003
DOI:
10.1371/journal.pone.0284135.g004
DOI:
10.1371/journal.pone.0284135.g005
DOI:
10.1371/journal.pone.0284135.g006
DOI:
10.1371/journal.pone.0284135.g007
DOI:
10.1371/journal.pone.0284135.g008
DOI:
10.1371/journal.pone.0284135.g009
DOI:
10.1371/journal.pone.0284135.g010
DOI:
10.1371/journal.pone.0284135.g011
DOI:
10.1371/journal.pone.0284135.g012
DOI:
10.1371/journal.pone.0284135.g013
DOI:
10.1371/journal.pone.0284135.t001
DOI:
10.1371/journal.pone.0284135.t002
DOI:
10.1371/journal.pone.0284135.t003
DOI:
10.1371/journal.pone.0284135.t004
DOI:
10.1371/journal.pone.0284135.s001
DOI:
10.1371/journal.pone.0284135.s002
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
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